Evaluation of the Immunoprotective Capacity of Five Vaccine Candidate Proteins against Avian Necrotic Enteritis and Impact on the Caecal Microbiota of Vaccinated Birds

SIMPLE SUMMARY: Necrotic enteritis is a complex gastrointestinal disease of broiler chickens that imposes a substantial economic burden on poultry producers worldwide. The causative agent is pathogenic strains of Clostridium perfringens type G. While numerous efforts to develop an effective vaccine against the disease were unsuccessful, a previous study by our group identified five potential vaccine candidate proteins. Herein, we assessed the relative contribution of the specific immunity raised by these proteins to protect broiler chickens against an experimental disease challenge and measured the impact on the intestinal microbiota. While it did not significantly impact the bacterial populations in the intestine, the protective capacity of the antibodies raised by the immunization of birds with the recombinant proteins should be further assessed using different necrotic enteritis induction models. ABSTRACT: Avian necrotic enteritis is an enteric disease of broiler chickens caused by certain pathogenic strains of Clostridium perfringens in combination with predisposing factors. A vaccine offering complete protection against the disease has not yet been commercialized. In a previous study, we produced five recombinant proteins predicted to be surface-exposed and unique to necrotic enteritis-causing C. perfringens and the immunogenicity of these potential vaccine candidates was assessed in broiler chickens. In the current work, the relative contribution of the antibodies raised by these putative antigens to protect broiler chickens was evaluated using an experimental necrotic enteritis induction model. Additionally, the link between the immune response elicited and the gut microbiota profiles in immunized birds subjected to infection with virulent C. perfringens was studied. The ELISA results showed that the IgY antibody titers in vaccinated birds on days 21 and 33 were significantly higher than those on days 7 and 14 and those in birds receiving the adjuvant alone, while the relative contribution of the specific immunity attributed to these antibodies could not be precisely determined using this experimental necrotic enteritis induction model. In addition, 16S rRNA gene amplicon sequencing showed that immunization of birds with recombinant proteins had a low impact on the chicken caecal microbiota.