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The Role of Hydrogen Sulfide in the Localization and Expression of p53 and Cell Death in the Nervous Tissue in Traumatic Brain Injury and Axotomy
Traumatic brain injury (TBI) is one of the leading causes of disability and death worldwide. It is characterized by various molecular–cellular events, with the main ones being apoptosis and damage to axons. To date, there are no clinically effective neuroprotective drugs. In this study, we examined...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10650615/ https://www.ncbi.nlm.nih.gov/pubmed/37958692 http://dx.doi.org/10.3390/ijms242115708 |
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author | Rodkin, Stanislav Nwosu, Chizaram Raevskaya, Margarita Khanukaev, Maxim Bekova, Khava Vasilieva, Inna Vishnyak, Diana Tolmacheva, Anastasia Efremova, Elena Gasanov, Mitkhat Tyurin, Anton |
author_facet | Rodkin, Stanislav Nwosu, Chizaram Raevskaya, Margarita Khanukaev, Maxim Bekova, Khava Vasilieva, Inna Vishnyak, Diana Tolmacheva, Anastasia Efremova, Elena Gasanov, Mitkhat Tyurin, Anton |
author_sort | Rodkin, Stanislav |
collection | PubMed |
description | Traumatic brain injury (TBI) is one of the leading causes of disability and death worldwide. It is characterized by various molecular–cellular events, with the main ones being apoptosis and damage to axons. To date, there are no clinically effective neuroprotective drugs. In this study, we examined the role of hydrogen sulfide (H(2)S) in the localization and expression of the key pro-apoptotic protein p53, as well as cell death in the nervous tissue in TBI and axotomy. We used a fast donor (sodium sulphide, Na(2)S) H(2)S and a classic inhibitor (aminooxyacetic acid, AOAA) of cystathionine β-synthase (CBS), which is a key enzyme in H(2)S synthesis. These studies were carried out on three models of neurotrauma in vertebrates and invertebrates. As a result, it was found that Na(2)S exhibits a pronounced neuroprotective effect that reduces the number of TUNEL-positive neurons and glial cells in TBI and apoptotic glia in axotomy. This effect could be realized through the Na(2)S-dependent decrease in the level of p53 in the cells of the nervous tissue of vertebrates and invertebrates, which we observed in our study. We also observed the opposite effect when using AOAA, which indicates the important role of CBS in the regulation of p53 expression and death of neurons and glial cells in TBI and axotomy. |
format | Online Article Text |
id | pubmed-10650615 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-106506152023-10-28 The Role of Hydrogen Sulfide in the Localization and Expression of p53 and Cell Death in the Nervous Tissue in Traumatic Brain Injury and Axotomy Rodkin, Stanislav Nwosu, Chizaram Raevskaya, Margarita Khanukaev, Maxim Bekova, Khava Vasilieva, Inna Vishnyak, Diana Tolmacheva, Anastasia Efremova, Elena Gasanov, Mitkhat Tyurin, Anton Int J Mol Sci Article Traumatic brain injury (TBI) is one of the leading causes of disability and death worldwide. It is characterized by various molecular–cellular events, with the main ones being apoptosis and damage to axons. To date, there are no clinically effective neuroprotective drugs. In this study, we examined the role of hydrogen sulfide (H(2)S) in the localization and expression of the key pro-apoptotic protein p53, as well as cell death in the nervous tissue in TBI and axotomy. We used a fast donor (sodium sulphide, Na(2)S) H(2)S and a classic inhibitor (aminooxyacetic acid, AOAA) of cystathionine β-synthase (CBS), which is a key enzyme in H(2)S synthesis. These studies were carried out on three models of neurotrauma in vertebrates and invertebrates. As a result, it was found that Na(2)S exhibits a pronounced neuroprotective effect that reduces the number of TUNEL-positive neurons and glial cells in TBI and apoptotic glia in axotomy. This effect could be realized through the Na(2)S-dependent decrease in the level of p53 in the cells of the nervous tissue of vertebrates and invertebrates, which we observed in our study. We also observed the opposite effect when using AOAA, which indicates the important role of CBS in the regulation of p53 expression and death of neurons and glial cells in TBI and axotomy. MDPI 2023-10-28 /pmc/articles/PMC10650615/ /pubmed/37958692 http://dx.doi.org/10.3390/ijms242115708 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rodkin, Stanislav Nwosu, Chizaram Raevskaya, Margarita Khanukaev, Maxim Bekova, Khava Vasilieva, Inna Vishnyak, Diana Tolmacheva, Anastasia Efremova, Elena Gasanov, Mitkhat Tyurin, Anton The Role of Hydrogen Sulfide in the Localization and Expression of p53 and Cell Death in the Nervous Tissue in Traumatic Brain Injury and Axotomy |
title | The Role of Hydrogen Sulfide in the Localization and Expression of p53 and Cell Death in the Nervous Tissue in Traumatic Brain Injury and Axotomy |
title_full | The Role of Hydrogen Sulfide in the Localization and Expression of p53 and Cell Death in the Nervous Tissue in Traumatic Brain Injury and Axotomy |
title_fullStr | The Role of Hydrogen Sulfide in the Localization and Expression of p53 and Cell Death in the Nervous Tissue in Traumatic Brain Injury and Axotomy |
title_full_unstemmed | The Role of Hydrogen Sulfide in the Localization and Expression of p53 and Cell Death in the Nervous Tissue in Traumatic Brain Injury and Axotomy |
title_short | The Role of Hydrogen Sulfide in the Localization and Expression of p53 and Cell Death in the Nervous Tissue in Traumatic Brain Injury and Axotomy |
title_sort | role of hydrogen sulfide in the localization and expression of p53 and cell death in the nervous tissue in traumatic brain injury and axotomy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10650615/ https://www.ncbi.nlm.nih.gov/pubmed/37958692 http://dx.doi.org/10.3390/ijms242115708 |
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