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DNA barcoding as new diagnostic tool to lethal plant poisoning in herbivorous mammals
Reliable identification of plant species in the digestive tract of a deceased animal often represents the major key to diagnose a lethal intoxication with poisonous plants in veterinary pathology. In many cases, identification of the species is challenging or even impossible because the diagnostic m...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10650979/ https://www.ncbi.nlm.nih.gov/pubmed/37967132 http://dx.doi.org/10.1371/journal.pone.0292275 |
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author | Schweikle, Sandra Häser, Annette Wetters, Sascha Raisin, Moses Greiner, Maica Rigbers, Kerstin Fischer, Ulrike Pietsch, Klaus Suntz, Michael Nick, Peter |
author_facet | Schweikle, Sandra Häser, Annette Wetters, Sascha Raisin, Moses Greiner, Maica Rigbers, Kerstin Fischer, Ulrike Pietsch, Klaus Suntz, Michael Nick, Peter |
author_sort | Schweikle, Sandra |
collection | PubMed |
description | Reliable identification of plant species in the digestive tract of a deceased animal often represents the major key to diagnose a lethal intoxication with poisonous plants in veterinary pathology. In many cases, identification of the species is challenging or even impossible because the diagnostic morphological features have been degraded, and because the interpretation of such features requires a considerable expertise in plant anatomy and biodiversity. The use of DNA barcoding markers can support or even replace classical morphological assessment. While these markers have been widely used for plant taxonomy, their forensic application to clarify causes of animal poisoning is novel. In addition, we use specific single-nucleotide polymorphisms as fingerprints. This allows for a clear decision even in cases, where the conventionally used statistical e-values remain ambiguous. In the current work, we explore the feasibility of this strategy in a couple of exemplary cases, either in concert with anatomical diagnostics, or in cases where visual species identification is not possible, or where chemical toxin detection methods are not well established, complex, time consuming and expensive. |
format | Online Article Text |
id | pubmed-10650979 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-106509792023-11-15 DNA barcoding as new diagnostic tool to lethal plant poisoning in herbivorous mammals Schweikle, Sandra Häser, Annette Wetters, Sascha Raisin, Moses Greiner, Maica Rigbers, Kerstin Fischer, Ulrike Pietsch, Klaus Suntz, Michael Nick, Peter PLoS One Research Article Reliable identification of plant species in the digestive tract of a deceased animal often represents the major key to diagnose a lethal intoxication with poisonous plants in veterinary pathology. In many cases, identification of the species is challenging or even impossible because the diagnostic morphological features have been degraded, and because the interpretation of such features requires a considerable expertise in plant anatomy and biodiversity. The use of DNA barcoding markers can support or even replace classical morphological assessment. While these markers have been widely used for plant taxonomy, their forensic application to clarify causes of animal poisoning is novel. In addition, we use specific single-nucleotide polymorphisms as fingerprints. This allows for a clear decision even in cases, where the conventionally used statistical e-values remain ambiguous. In the current work, we explore the feasibility of this strategy in a couple of exemplary cases, either in concert with anatomical diagnostics, or in cases where visual species identification is not possible, or where chemical toxin detection methods are not well established, complex, time consuming and expensive. Public Library of Science 2023-11-15 /pmc/articles/PMC10650979/ /pubmed/37967132 http://dx.doi.org/10.1371/journal.pone.0292275 Text en © 2023 Schweikle et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Schweikle, Sandra Häser, Annette Wetters, Sascha Raisin, Moses Greiner, Maica Rigbers, Kerstin Fischer, Ulrike Pietsch, Klaus Suntz, Michael Nick, Peter DNA barcoding as new diagnostic tool to lethal plant poisoning in herbivorous mammals |
title | DNA barcoding as new diagnostic tool to lethal plant poisoning in herbivorous mammals |
title_full | DNA barcoding as new diagnostic tool to lethal plant poisoning in herbivorous mammals |
title_fullStr | DNA barcoding as new diagnostic tool to lethal plant poisoning in herbivorous mammals |
title_full_unstemmed | DNA barcoding as new diagnostic tool to lethal plant poisoning in herbivorous mammals |
title_short | DNA barcoding as new diagnostic tool to lethal plant poisoning in herbivorous mammals |
title_sort | dna barcoding as new diagnostic tool to lethal plant poisoning in herbivorous mammals |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10650979/ https://www.ncbi.nlm.nih.gov/pubmed/37967132 http://dx.doi.org/10.1371/journal.pone.0292275 |
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