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Production of high protein yeast using enzymatically liquefied almond hulls

Animal feed ingredients, especially those abundant in high quality protein, are the most expensive component of livestock production. Sustainable alternative feedstocks may be sourced from abundant, low value agricultural byproducts. California almond production generates nearly 3 Mtons of biomass p...

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Autores principales: Sitepu, Irnayuli R., Hitomi, Alex, Wu, Wayne, Wu, Angela, Jeoh, Tina, Boundy-Mills, Kyria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10651018/
https://www.ncbi.nlm.nih.gov/pubmed/37967097
http://dx.doi.org/10.1371/journal.pone.0293085
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author Sitepu, Irnayuli R.
Hitomi, Alex
Wu, Wayne
Wu, Angela
Jeoh, Tina
Boundy-Mills, Kyria
author_facet Sitepu, Irnayuli R.
Hitomi, Alex
Wu, Wayne
Wu, Angela
Jeoh, Tina
Boundy-Mills, Kyria
author_sort Sitepu, Irnayuli R.
collection PubMed
description Animal feed ingredients, especially those abundant in high quality protein, are the most expensive component of livestock production. Sustainable alternative feedstocks may be sourced from abundant, low value agricultural byproducts. California almond production generates nearly 3 Mtons of biomass per year with about 50% in the form of hulls. Almond hulls are a low-value byproduct currently used primarily for animal feed for dairy cattle. However, the protein and essential amino acid content are low, at ~30% d.b.. The purpose of this study was to improve the protein content and quality using yeast. To achieve this, the almond hulls were liquefied to liberate soluble and structural sugars. A multi-phase screening approach was used to identify yeasts that can consume a large proportion of the sugars in almond hulls while accumulating high concentrations of amino acids essential for livestock feed. Compositional analysis showed that almond hulls are rich in polygalacturonic acid (pectin) and soluble sucrose. A pectinase-assisted process was optimized to liquefy and release soluble sugars from almond hulls. The resulting almond hull slurry containing solubilized sugars was subsequently used to grow high-protein yeasts that could consume nutrients in almond hulls while accumulating high concentrations of high-quality protein rich in essential amino acids needed for livestock feed, yielding a process that would produce 72 mg protein/g almond hull. Further work is needed to achieve conversion of galacturonic acid to yeast cell biomass.
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spelling pubmed-106510182023-11-15 Production of high protein yeast using enzymatically liquefied almond hulls Sitepu, Irnayuli R. Hitomi, Alex Wu, Wayne Wu, Angela Jeoh, Tina Boundy-Mills, Kyria PLoS One Research Article Animal feed ingredients, especially those abundant in high quality protein, are the most expensive component of livestock production. Sustainable alternative feedstocks may be sourced from abundant, low value agricultural byproducts. California almond production generates nearly 3 Mtons of biomass per year with about 50% in the form of hulls. Almond hulls are a low-value byproduct currently used primarily for animal feed for dairy cattle. However, the protein and essential amino acid content are low, at ~30% d.b.. The purpose of this study was to improve the protein content and quality using yeast. To achieve this, the almond hulls were liquefied to liberate soluble and structural sugars. A multi-phase screening approach was used to identify yeasts that can consume a large proportion of the sugars in almond hulls while accumulating high concentrations of amino acids essential for livestock feed. Compositional analysis showed that almond hulls are rich in polygalacturonic acid (pectin) and soluble sucrose. A pectinase-assisted process was optimized to liquefy and release soluble sugars from almond hulls. The resulting almond hull slurry containing solubilized sugars was subsequently used to grow high-protein yeasts that could consume nutrients in almond hulls while accumulating high concentrations of high-quality protein rich in essential amino acids needed for livestock feed, yielding a process that would produce 72 mg protein/g almond hull. Further work is needed to achieve conversion of galacturonic acid to yeast cell biomass. Public Library of Science 2023-11-15 /pmc/articles/PMC10651018/ /pubmed/37967097 http://dx.doi.org/10.1371/journal.pone.0293085 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Sitepu, Irnayuli R.
Hitomi, Alex
Wu, Wayne
Wu, Angela
Jeoh, Tina
Boundy-Mills, Kyria
Production of high protein yeast using enzymatically liquefied almond hulls
title Production of high protein yeast using enzymatically liquefied almond hulls
title_full Production of high protein yeast using enzymatically liquefied almond hulls
title_fullStr Production of high protein yeast using enzymatically liquefied almond hulls
title_full_unstemmed Production of high protein yeast using enzymatically liquefied almond hulls
title_short Production of high protein yeast using enzymatically liquefied almond hulls
title_sort production of high protein yeast using enzymatically liquefied almond hulls
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10651018/
https://www.ncbi.nlm.nih.gov/pubmed/37967097
http://dx.doi.org/10.1371/journal.pone.0293085
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