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Directed evolution rice genes with randomly multiplexed sgRNAs assembly of base editors

CRISPR‐based directed evolution is an effective breeding biotechnology to improve agronomic traits in plants. However, its gene diversification is still limited using individual single guide RNA. We described here a multiplexed orthogonal base editor (MoBE), and a randomly multiplexed sgRNAs assembl...

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Detalles Bibliográficos
Autores principales:	Zhang, Ao, Shan, Tiaofeng, Sun, Yan, Chen, Zhipeng, Hu, Jianjian, Hu, Zhichao, Ming, Ziheng, Zhu, Zhitao, Li, Xue, He, Jun, Liu, Shijia, Jiang, Ling, Dong, Xiaoou, Wu, Yufeng, Wang, Yanpeng, Liu, Yuqiang, Li, Chao, Wan, Jianmin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2023
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10651138/ https://www.ncbi.nlm.nih.gov/pubmed/37571976 http://dx.doi.org/10.1111/pbi.14156

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