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Immunogenetics, sylvatic plague and its vectors: insights from the pathogen reservoir Mastomys natalensis in Tanzania
Yersinia pestis is a historically important vector-borne pathogen causing plague in humans and other mammals. Contemporary zoonotic infections with Y. pestis still occur in sub-Saharan Africa, including Tanzania and Madagascar, but receive relatively little attention. Thus, the role of wildlife rese...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10651713/ https://www.ncbi.nlm.nih.gov/pubmed/37853246 http://dx.doi.org/10.1007/s00251-023-01323-7 |
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author | Haikukutu, Lavinia Lyaku, Japhet R. Lyimo, Charles M. Eiseb, Seth J. Makundi, Rhodes H. Olayemi, Ayodeji Wilhelm, Kerstin Müller-Klein, Nadine Schmid, Dominik W. Fleischer, Ramona Sommer, Simone |
author_facet | Haikukutu, Lavinia Lyaku, Japhet R. Lyimo, Charles M. Eiseb, Seth J. Makundi, Rhodes H. Olayemi, Ayodeji Wilhelm, Kerstin Müller-Klein, Nadine Schmid, Dominik W. Fleischer, Ramona Sommer, Simone |
author_sort | Haikukutu, Lavinia |
collection | PubMed |
description | Yersinia pestis is a historically important vector-borne pathogen causing plague in humans and other mammals. Contemporary zoonotic infections with Y. pestis still occur in sub-Saharan Africa, including Tanzania and Madagascar, but receive relatively little attention. Thus, the role of wildlife reservoirs in maintaining sylvatic plague and spillover risks to humans is largely unknown. The multimammate rodent Mastomys natalensis is the most abundant and widespread rodent in peri-domestic areas in Tanzania, where it plays a major role as a Y. pestis reservoir in endemic foci. Yet, how M. natalensis’ immunogenetics contributes to the maintenance of plague has not been investigated to date. Here, we surveyed wild M. natalensis for Y. pestis vectors, i.e., fleas, and tested for the presence of antibodies against Y. pestis using enzyme-linked immunosorbent assays (ELISA) in areas known to be endemic or without previous records of Y. pestis in Tanzania. We characterized the allelic and functional (i.e., supertype) diversity of the major histocompatibility complex (MHC class II) of M. natalensis and investigated links to Y. pestis vectors and infections. We detected antibodies against Y. pestis in rodents inhabiting both endemic areas and areas considered non-endemic. Of the 111 nucleotide MHC alleles, only DRB*016 was associated with an increased infestation with the flea Xenopsylla. Surprisingly, we found no link between MHC alleles or supertypes and antibodies of Y. pestis. Our findings hint, however, at local adaptations towards Y. pestis vectors, an observation that more exhaustive sampling could unwind in the future. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00251-023-01323-7. |
format | Online Article Text |
id | pubmed-10651713 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-106517132023-10-19 Immunogenetics, sylvatic plague and its vectors: insights from the pathogen reservoir Mastomys natalensis in Tanzania Haikukutu, Lavinia Lyaku, Japhet R. Lyimo, Charles M. Eiseb, Seth J. Makundi, Rhodes H. Olayemi, Ayodeji Wilhelm, Kerstin Müller-Klein, Nadine Schmid, Dominik W. Fleischer, Ramona Sommer, Simone Immunogenetics Original Article Yersinia pestis is a historically important vector-borne pathogen causing plague in humans and other mammals. Contemporary zoonotic infections with Y. pestis still occur in sub-Saharan Africa, including Tanzania and Madagascar, but receive relatively little attention. Thus, the role of wildlife reservoirs in maintaining sylvatic plague and spillover risks to humans is largely unknown. The multimammate rodent Mastomys natalensis is the most abundant and widespread rodent in peri-domestic areas in Tanzania, where it plays a major role as a Y. pestis reservoir in endemic foci. Yet, how M. natalensis’ immunogenetics contributes to the maintenance of plague has not been investigated to date. Here, we surveyed wild M. natalensis for Y. pestis vectors, i.e., fleas, and tested for the presence of antibodies against Y. pestis using enzyme-linked immunosorbent assays (ELISA) in areas known to be endemic or without previous records of Y. pestis in Tanzania. We characterized the allelic and functional (i.e., supertype) diversity of the major histocompatibility complex (MHC class II) of M. natalensis and investigated links to Y. pestis vectors and infections. We detected antibodies against Y. pestis in rodents inhabiting both endemic areas and areas considered non-endemic. Of the 111 nucleotide MHC alleles, only DRB*016 was associated with an increased infestation with the flea Xenopsylla. Surprisingly, we found no link between MHC alleles or supertypes and antibodies of Y. pestis. Our findings hint, however, at local adaptations towards Y. pestis vectors, an observation that more exhaustive sampling could unwind in the future. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00251-023-01323-7. Springer Berlin Heidelberg 2023-10-19 2023 /pmc/articles/PMC10651713/ /pubmed/37853246 http://dx.doi.org/10.1007/s00251-023-01323-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Haikukutu, Lavinia Lyaku, Japhet R. Lyimo, Charles M. Eiseb, Seth J. Makundi, Rhodes H. Olayemi, Ayodeji Wilhelm, Kerstin Müller-Klein, Nadine Schmid, Dominik W. Fleischer, Ramona Sommer, Simone Immunogenetics, sylvatic plague and its vectors: insights from the pathogen reservoir Mastomys natalensis in Tanzania |
title | Immunogenetics, sylvatic plague and its vectors: insights from the pathogen reservoir Mastomys natalensis in Tanzania |
title_full | Immunogenetics, sylvatic plague and its vectors: insights from the pathogen reservoir Mastomys natalensis in Tanzania |
title_fullStr | Immunogenetics, sylvatic plague and its vectors: insights from the pathogen reservoir Mastomys natalensis in Tanzania |
title_full_unstemmed | Immunogenetics, sylvatic plague and its vectors: insights from the pathogen reservoir Mastomys natalensis in Tanzania |
title_short | Immunogenetics, sylvatic plague and its vectors: insights from the pathogen reservoir Mastomys natalensis in Tanzania |
title_sort | immunogenetics, sylvatic plague and its vectors: insights from the pathogen reservoir mastomys natalensis in tanzania |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10651713/ https://www.ncbi.nlm.nih.gov/pubmed/37853246 http://dx.doi.org/10.1007/s00251-023-01323-7 |
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