Cargando…
Microbial associates of an endemic Mediterranean seagrass enhance the access of the host and the surrounding seawater to inorganic nitrogen under ocean acidification
Seagrasses are important primary producers in oceans worldwide. They live in shallow coastal waters that are experiencing carbon dioxide enrichment and ocean acidification. Posidonia oceanica, an endemic seagrass species that dominates the Mediterranean Sea, achieves high abundances in seawater with...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10651887/ https://www.ncbi.nlm.nih.gov/pubmed/37968499 http://dx.doi.org/10.1038/s41598-023-47126-4 |
_version_ | 1785147647973130240 |
---|---|
author | Pfister, Catherine A. Cardini, Ulisse Mirasole, Alice Montilla, Luis M. Veseli, Iva Gattuso, Jean-Pierre Teixido, Nuria |
author_facet | Pfister, Catherine A. Cardini, Ulisse Mirasole, Alice Montilla, Luis M. Veseli, Iva Gattuso, Jean-Pierre Teixido, Nuria |
author_sort | Pfister, Catherine A. |
collection | PubMed |
description | Seagrasses are important primary producers in oceans worldwide. They live in shallow coastal waters that are experiencing carbon dioxide enrichment and ocean acidification. Posidonia oceanica, an endemic seagrass species that dominates the Mediterranean Sea, achieves high abundances in seawater with relatively low concentrations of dissolved inorganic nitrogen. Here we tested whether microbial metabolisms associated with P. oceanica and surrounding seawater enhance seagrass access to nitrogen. Using stable isotope enrichments of intact seagrass with amino acids, we showed that ammonification by free-living and seagrass-associated microbes produce ammonium that is likely used by seagrass and surrounding particulate organic matter. Metagenomic analysis of the epiphytic biofilm on the blades and rhizomes support the ubiquity of microbial ammonification genes in this system. Further, we leveraged the presence of natural carbon dioxide vents and show that the presence of P. oceanica enhanced the uptake of nitrogen by water column particulate organic matter, increasing carbon fixation by a factor of 8.6–17.4 with the greatest effect at CO(2) vent sites. However, microbial ammonification was reduced at lower pH, suggesting that future ocean climate change will compromise this microbial process. Thus, the seagrass holobiont enhances water column productivity, even in the context of ocean acidification. |
format | Online Article Text |
id | pubmed-10651887 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-106518872023-11-15 Microbial associates of an endemic Mediterranean seagrass enhance the access of the host and the surrounding seawater to inorganic nitrogen under ocean acidification Pfister, Catherine A. Cardini, Ulisse Mirasole, Alice Montilla, Luis M. Veseli, Iva Gattuso, Jean-Pierre Teixido, Nuria Sci Rep Article Seagrasses are important primary producers in oceans worldwide. They live in shallow coastal waters that are experiencing carbon dioxide enrichment and ocean acidification. Posidonia oceanica, an endemic seagrass species that dominates the Mediterranean Sea, achieves high abundances in seawater with relatively low concentrations of dissolved inorganic nitrogen. Here we tested whether microbial metabolisms associated with P. oceanica and surrounding seawater enhance seagrass access to nitrogen. Using stable isotope enrichments of intact seagrass with amino acids, we showed that ammonification by free-living and seagrass-associated microbes produce ammonium that is likely used by seagrass and surrounding particulate organic matter. Metagenomic analysis of the epiphytic biofilm on the blades and rhizomes support the ubiquity of microbial ammonification genes in this system. Further, we leveraged the presence of natural carbon dioxide vents and show that the presence of P. oceanica enhanced the uptake of nitrogen by water column particulate organic matter, increasing carbon fixation by a factor of 8.6–17.4 with the greatest effect at CO(2) vent sites. However, microbial ammonification was reduced at lower pH, suggesting that future ocean climate change will compromise this microbial process. Thus, the seagrass holobiont enhances water column productivity, even in the context of ocean acidification. Nature Publishing Group UK 2023-11-15 /pmc/articles/PMC10651887/ /pubmed/37968499 http://dx.doi.org/10.1038/s41598-023-47126-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Pfister, Catherine A. Cardini, Ulisse Mirasole, Alice Montilla, Luis M. Veseli, Iva Gattuso, Jean-Pierre Teixido, Nuria Microbial associates of an endemic Mediterranean seagrass enhance the access of the host and the surrounding seawater to inorganic nitrogen under ocean acidification |
title | Microbial associates of an endemic Mediterranean seagrass enhance the access of the host and the surrounding seawater to inorganic nitrogen under ocean acidification |
title_full | Microbial associates of an endemic Mediterranean seagrass enhance the access of the host and the surrounding seawater to inorganic nitrogen under ocean acidification |
title_fullStr | Microbial associates of an endemic Mediterranean seagrass enhance the access of the host and the surrounding seawater to inorganic nitrogen under ocean acidification |
title_full_unstemmed | Microbial associates of an endemic Mediterranean seagrass enhance the access of the host and the surrounding seawater to inorganic nitrogen under ocean acidification |
title_short | Microbial associates of an endemic Mediterranean seagrass enhance the access of the host and the surrounding seawater to inorganic nitrogen under ocean acidification |
title_sort | microbial associates of an endemic mediterranean seagrass enhance the access of the host and the surrounding seawater to inorganic nitrogen under ocean acidification |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10651887/ https://www.ncbi.nlm.nih.gov/pubmed/37968499 http://dx.doi.org/10.1038/s41598-023-47126-4 |
work_keys_str_mv | AT pfistercatherinea microbialassociatesofanendemicmediterraneanseagrassenhancetheaccessofthehostandthesurroundingseawatertoinorganicnitrogenunderoceanacidification AT cardiniulisse microbialassociatesofanendemicmediterraneanseagrassenhancetheaccessofthehostandthesurroundingseawatertoinorganicnitrogenunderoceanacidification AT mirasolealice microbialassociatesofanendemicmediterraneanseagrassenhancetheaccessofthehostandthesurroundingseawatertoinorganicnitrogenunderoceanacidification AT montillaluism microbialassociatesofanendemicmediterraneanseagrassenhancetheaccessofthehostandthesurroundingseawatertoinorganicnitrogenunderoceanacidification AT veseliiva microbialassociatesofanendemicmediterraneanseagrassenhancetheaccessofthehostandthesurroundingseawatertoinorganicnitrogenunderoceanacidification AT gattusojeanpierre microbialassociatesofanendemicmediterraneanseagrassenhancetheaccessofthehostandthesurroundingseawatertoinorganicnitrogenunderoceanacidification AT teixidonuria microbialassociatesofanendemicmediterraneanseagrassenhancetheaccessofthehostandthesurroundingseawatertoinorganicnitrogenunderoceanacidification |