Chitin degradation by Synechococcus WH7803

Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures—yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-f...

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Autores principales: Capovilla, Giovanna, Castro, Kurt G., Collani, Silvio, Kearney, Sean M., Kehoe, David M., Chisholm, Sallie W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10651935/
https://www.ncbi.nlm.nih.gov/pubmed/37968300
http://dx.doi.org/10.1038/s41598-023-47332-0
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author Capovilla, Giovanna
Castro, Kurt G.
Collani, Silvio
Kearney, Sean M.
Kehoe, David M.
Chisholm, Sallie W.
author_facet Capovilla, Giovanna
Castro, Kurt G.
Collani, Silvio
Kearney, Sean M.
Kehoe, David M.
Chisholm, Sallie W.
author_sort Capovilla, Giovanna
collection PubMed
description Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures—yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type.
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spelling pubmed-106519352023-11-15 Chitin degradation by Synechococcus WH7803 Capovilla, Giovanna Castro, Kurt G. Collani, Silvio Kearney, Sean M. Kehoe, David M. Chisholm, Sallie W. Sci Rep Article Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures—yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type. Nature Publishing Group UK 2023-11-15 /pmc/articles/PMC10651935/ /pubmed/37968300 http://dx.doi.org/10.1038/s41598-023-47332-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Capovilla, Giovanna
Castro, Kurt G.
Collani, Silvio
Kearney, Sean M.
Kehoe, David M.
Chisholm, Sallie W.
Chitin degradation by Synechococcus WH7803
title Chitin degradation by Synechococcus WH7803
title_full Chitin degradation by Synechococcus WH7803
title_fullStr Chitin degradation by Synechococcus WH7803
title_full_unstemmed Chitin degradation by Synechococcus WH7803
title_short Chitin degradation by Synechococcus WH7803
title_sort chitin degradation by synechococcus wh7803
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10651935/
https://www.ncbi.nlm.nih.gov/pubmed/37968300
http://dx.doi.org/10.1038/s41598-023-47332-0
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