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Exosomal miR‐6733‐5p mediates cross‐talk between glioblastoma stem cells and macrophages and promotes glioblastoma multiform progression synergistically
AIM: Exosomal miRNAs derived from glioblastoma stem cells (GSCs) are important mediators of immunosuppressive microenvironment formation in glioblastoma multiform (GBM), especially in M2‐like polarization of tumor‐associated macrophages (TAMs). However, the exact mechanisms by which GSCs‐derived exo...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10651992/ https://www.ncbi.nlm.nih.gov/pubmed/37309294 http://dx.doi.org/10.1111/cns.14296 |
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author | Huang, Shilu Liu, Liang Xu, Zhipeng Liu, Xinglei Wu, Anyi Zhang, Xiaopei Li, Zengyang Li, Suwen Li, Yongdong Yuan, Jiaqi Cheng, Shan Li, Haoran Dong, Jun |
author_facet | Huang, Shilu Liu, Liang Xu, Zhipeng Liu, Xinglei Wu, Anyi Zhang, Xiaopei Li, Zengyang Li, Suwen Li, Yongdong Yuan, Jiaqi Cheng, Shan Li, Haoran Dong, Jun |
author_sort | Huang, Shilu |
collection | PubMed |
description | AIM: Exosomal miRNAs derived from glioblastoma stem cells (GSCs) are important mediators of immunosuppressive microenvironment formation in glioblastoma multiform (GBM), especially in M2‐like polarization of tumor‐associated macrophages (TAMs). However, the exact mechanisms by which GSCs‐derived exosomes (GSCs‐exo) facilitate the remodeling of the immunosuppressive microenvironment of GBM have not been elucidated. METHODS: Transmission electron microscopy (TME) and nanoparticle tracking analysis (NTA) were applied to verify the existence of GSCs‐derived exosomes. Sphere formation assays, flow cytometry, and tumor xenograft transplantation assays were performed to identify the exact roles of exosomal miR‐6733‐5p. Then, the mechanisms of miR‐6733‐5p and its downstream target gene regulating crosstalk between GSCs cells and M2 macrophages were further investigated. RESULTS: GSCs‐derived exosomal miR‐6733‐5p induce macrophage M2 polarization of TAMs by positively targeting IGF2BP3 to activate the AKT signaling pathway, which further facilitates the self‐renewal and stemness of GSCs. CONCLUSION: GSCs secrete miR‐6733‐5p‐rich exosomes to induce M2‐like polarization of macrophages, as well as enhance GSCs stemness and promote malignant behaviors of GBM through IGF2BP3 activated AKT pathway. Targeting GSCs exosomal miR‐6733‐5p may provide a potential new strategy against GBM. |
format | Online Article Text |
id | pubmed-10651992 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-106519922023-06-12 Exosomal miR‐6733‐5p mediates cross‐talk between glioblastoma stem cells and macrophages and promotes glioblastoma multiform progression synergistically Huang, Shilu Liu, Liang Xu, Zhipeng Liu, Xinglei Wu, Anyi Zhang, Xiaopei Li, Zengyang Li, Suwen Li, Yongdong Yuan, Jiaqi Cheng, Shan Li, Haoran Dong, Jun CNS Neurosci Ther Original Articles AIM: Exosomal miRNAs derived from glioblastoma stem cells (GSCs) are important mediators of immunosuppressive microenvironment formation in glioblastoma multiform (GBM), especially in M2‐like polarization of tumor‐associated macrophages (TAMs). However, the exact mechanisms by which GSCs‐derived exosomes (GSCs‐exo) facilitate the remodeling of the immunosuppressive microenvironment of GBM have not been elucidated. METHODS: Transmission electron microscopy (TME) and nanoparticle tracking analysis (NTA) were applied to verify the existence of GSCs‐derived exosomes. Sphere formation assays, flow cytometry, and tumor xenograft transplantation assays were performed to identify the exact roles of exosomal miR‐6733‐5p. Then, the mechanisms of miR‐6733‐5p and its downstream target gene regulating crosstalk between GSCs cells and M2 macrophages were further investigated. RESULTS: GSCs‐derived exosomal miR‐6733‐5p induce macrophage M2 polarization of TAMs by positively targeting IGF2BP3 to activate the AKT signaling pathway, which further facilitates the self‐renewal and stemness of GSCs. CONCLUSION: GSCs secrete miR‐6733‐5p‐rich exosomes to induce M2‐like polarization of macrophages, as well as enhance GSCs stemness and promote malignant behaviors of GBM through IGF2BP3 activated AKT pathway. Targeting GSCs exosomal miR‐6733‐5p may provide a potential new strategy against GBM. John Wiley and Sons Inc. 2023-06-12 /pmc/articles/PMC10651992/ /pubmed/37309294 http://dx.doi.org/10.1111/cns.14296 Text en © 2023 The Authors. CNS Neuroscience & Therapeutics published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Huang, Shilu Liu, Liang Xu, Zhipeng Liu, Xinglei Wu, Anyi Zhang, Xiaopei Li, Zengyang Li, Suwen Li, Yongdong Yuan, Jiaqi Cheng, Shan Li, Haoran Dong, Jun Exosomal miR‐6733‐5p mediates cross‐talk between glioblastoma stem cells and macrophages and promotes glioblastoma multiform progression synergistically |
title | Exosomal miR‐6733‐5p mediates cross‐talk between glioblastoma stem cells and macrophages and promotes glioblastoma multiform progression synergistically |
title_full | Exosomal miR‐6733‐5p mediates cross‐talk between glioblastoma stem cells and macrophages and promotes glioblastoma multiform progression synergistically |
title_fullStr | Exosomal miR‐6733‐5p mediates cross‐talk between glioblastoma stem cells and macrophages and promotes glioblastoma multiform progression synergistically |
title_full_unstemmed | Exosomal miR‐6733‐5p mediates cross‐talk between glioblastoma stem cells and macrophages and promotes glioblastoma multiform progression synergistically |
title_short | Exosomal miR‐6733‐5p mediates cross‐talk between glioblastoma stem cells and macrophages and promotes glioblastoma multiform progression synergistically |
title_sort | exosomal mir‐6733‐5p mediates cross‐talk between glioblastoma stem cells and macrophages and promotes glioblastoma multiform progression synergistically |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10651992/ https://www.ncbi.nlm.nih.gov/pubmed/37309294 http://dx.doi.org/10.1111/cns.14296 |
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