Protocol for isolation of signet ring cells from human gastric mucosa

More than 90% of individuals with germline pathogenic CDH1 variants will harbor occult, microscopic foci of signet ring cell carcinomas capable of progressing to advanced diffuse-type gastric cancer. Here, we present a protocol for high viability suspension of signet ring cells from human gastric ti...

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Detalles Bibliográficos
Autores principales: Samaranayake, Sarah G., Gamble, Lauren A., Bowden, Cassidy, Green, Benjamin L., Gallanis, Amber F., Akbulut, Dilara, Shah, Niharika, Hernandez, Jonathan M., Davis, Jeremy L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10652202/
https://www.ncbi.nlm.nih.gov/pubmed/37925632
http://dx.doi.org/10.1016/j.xpro.2023.102695
Descripción
Sumario:More than 90% of individuals with germline pathogenic CDH1 variants will harbor occult, microscopic foci of signet ring cell carcinomas capable of progressing to advanced diffuse-type gastric cancer. Here, we present a protocol for high viability suspension of signet ring cells from human gastric tissue. We describe the steps for gastric mucosa isolation and tissue dissociation. We then detail procedures for embedding cells into HistoGel for immunohistochemistry staining and additional applications such as flow cytometry and single-cell sequencing.

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