Protocol for isolation of signet ring cells from human gastric mucosa

More than 90% of individuals with germline pathogenic CDH1 variants will harbor occult, microscopic foci of signet ring cell carcinomas capable of progressing to advanced diffuse-type gastric cancer. Here, we present a protocol for high viability suspension of signet ring cells from human gastric ti...

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Autores principales: Samaranayake, Sarah G., Gamble, Lauren A., Bowden, Cassidy, Green, Benjamin L., Gallanis, Amber F., Akbulut, Dilara, Shah, Niharika, Hernandez, Jonathan M., Davis, Jeremy L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10652202/
https://www.ncbi.nlm.nih.gov/pubmed/37925632
http://dx.doi.org/10.1016/j.xpro.2023.102695
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author Samaranayake, Sarah G.
Gamble, Lauren A.
Bowden, Cassidy
Green, Benjamin L.
Gallanis, Amber F.
Akbulut, Dilara
Shah, Niharika
Hernandez, Jonathan M.
Davis, Jeremy L.
author_facet Samaranayake, Sarah G.
Gamble, Lauren A.
Bowden, Cassidy
Green, Benjamin L.
Gallanis, Amber F.
Akbulut, Dilara
Shah, Niharika
Hernandez, Jonathan M.
Davis, Jeremy L.
author_sort Samaranayake, Sarah G.
collection PubMed
description More than 90% of individuals with germline pathogenic CDH1 variants will harbor occult, microscopic foci of signet ring cell carcinomas capable of progressing to advanced diffuse-type gastric cancer. Here, we present a protocol for high viability suspension of signet ring cells from human gastric tissue. We describe the steps for gastric mucosa isolation and tissue dissociation. We then detail procedures for embedding cells into HistoGel for immunohistochemistry staining and additional applications such as flow cytometry and single-cell sequencing.
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spelling pubmed-106522022023-11-04 Protocol for isolation of signet ring cells from human gastric mucosa Samaranayake, Sarah G. Gamble, Lauren A. Bowden, Cassidy Green, Benjamin L. Gallanis, Amber F. Akbulut, Dilara Shah, Niharika Hernandez, Jonathan M. Davis, Jeremy L. STAR Protoc Protocol More than 90% of individuals with germline pathogenic CDH1 variants will harbor occult, microscopic foci of signet ring cell carcinomas capable of progressing to advanced diffuse-type gastric cancer. Here, we present a protocol for high viability suspension of signet ring cells from human gastric tissue. We describe the steps for gastric mucosa isolation and tissue dissociation. We then detail procedures for embedding cells into HistoGel for immunohistochemistry staining and additional applications such as flow cytometry and single-cell sequencing. Elsevier 2023-11-04 /pmc/articles/PMC10652202/ /pubmed/37925632 http://dx.doi.org/10.1016/j.xpro.2023.102695 Text en https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Samaranayake, Sarah G.
Gamble, Lauren A.
Bowden, Cassidy
Green, Benjamin L.
Gallanis, Amber F.
Akbulut, Dilara
Shah, Niharika
Hernandez, Jonathan M.
Davis, Jeremy L.
Protocol for isolation of signet ring cells from human gastric mucosa
title Protocol for isolation of signet ring cells from human gastric mucosa
title_full Protocol for isolation of signet ring cells from human gastric mucosa
title_fullStr Protocol for isolation of signet ring cells from human gastric mucosa
title_full_unstemmed Protocol for isolation of signet ring cells from human gastric mucosa
title_short Protocol for isolation of signet ring cells from human gastric mucosa
title_sort protocol for isolation of signet ring cells from human gastric mucosa
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10652202/
https://www.ncbi.nlm.nih.gov/pubmed/37925632
http://dx.doi.org/10.1016/j.xpro.2023.102695
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