Establishing a mouse model of lung metastases using ultrasound-guided right heart ventricle injection

We report a technique to generate a murine model of lung metastases by selectively injecting tumor cells into the right heart ventricle under ultrasound guidance. First, we describe cell preparation and reference animal preparation as previously described. We then detail the technique using a previo...

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Detalles Bibliográficos
Autores principales: Labora, Amanda, Creech, Amanda, Lee, Hailey, Tabornal, Erin, Radu, Caius, Donahue, Timothy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10652205/
https://www.ncbi.nlm.nih.gov/pubmed/37925631
http://dx.doi.org/10.1016/j.xpro.2023.102698
Descripción
Sumario:We report a technique to generate a murine model of lung metastases by selectively injecting tumor cells into the right heart ventricle under ultrasound guidance. First, we describe cell preparation and reference animal preparation as previously described. We then detail the technique using a previously described 3D-printed instrument stabilization device. Finally, we describe tumor growth surveillance using bioluminescent imaging. For complete details on the use and execution of this protocol, please refer to Labora et al.(1)

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