Establishing a mouse model of lung metastases using ultrasound-guided right heart ventricle injection

We report a technique to generate a murine model of lung metastases by selectively injecting tumor cells into the right heart ventricle under ultrasound guidance. First, we describe cell preparation and reference animal preparation as previously described. We then detail the technique using a previo...

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Detalles Bibliográficos
Autores principales: Labora, Amanda, Creech, Amanda, Lee, Hailey, Tabornal, Erin, Radu, Caius, Donahue, Timothy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10652205/
https://www.ncbi.nlm.nih.gov/pubmed/37925631
http://dx.doi.org/10.1016/j.xpro.2023.102698
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author Labora, Amanda
Creech, Amanda
Lee, Hailey
Tabornal, Erin
Radu, Caius
Donahue, Timothy
author_facet Labora, Amanda
Creech, Amanda
Lee, Hailey
Tabornal, Erin
Radu, Caius
Donahue, Timothy
author_sort Labora, Amanda
collection PubMed
description We report a technique to generate a murine model of lung metastases by selectively injecting tumor cells into the right heart ventricle under ultrasound guidance. First, we describe cell preparation and reference animal preparation as previously described. We then detail the technique using a previously described 3D-printed instrument stabilization device. Finally, we describe tumor growth surveillance using bioluminescent imaging. For complete details on the use and execution of this protocol, please refer to Labora et al.(1)
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spelling pubmed-106522052023-11-04 Establishing a mouse model of lung metastases using ultrasound-guided right heart ventricle injection Labora, Amanda Creech, Amanda Lee, Hailey Tabornal, Erin Radu, Caius Donahue, Timothy STAR Protoc Protocol We report a technique to generate a murine model of lung metastases by selectively injecting tumor cells into the right heart ventricle under ultrasound guidance. First, we describe cell preparation and reference animal preparation as previously described. We then detail the technique using a previously described 3D-printed instrument stabilization device. Finally, we describe tumor growth surveillance using bioluminescent imaging. For complete details on the use and execution of this protocol, please refer to Labora et al.(1) Elsevier 2023-11-04 /pmc/articles/PMC10652205/ /pubmed/37925631 http://dx.doi.org/10.1016/j.xpro.2023.102698 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Labora, Amanda
Creech, Amanda
Lee, Hailey
Tabornal, Erin
Radu, Caius
Donahue, Timothy
Establishing a mouse model of lung metastases using ultrasound-guided right heart ventricle injection
title Establishing a mouse model of lung metastases using ultrasound-guided right heart ventricle injection
title_full Establishing a mouse model of lung metastases using ultrasound-guided right heart ventricle injection
title_fullStr Establishing a mouse model of lung metastases using ultrasound-guided right heart ventricle injection
title_full_unstemmed Establishing a mouse model of lung metastases using ultrasound-guided right heart ventricle injection
title_short Establishing a mouse model of lung metastases using ultrasound-guided right heart ventricle injection
title_sort establishing a mouse model of lung metastases using ultrasound-guided right heart ventricle injection
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10652205/
https://www.ncbi.nlm.nih.gov/pubmed/37925631
http://dx.doi.org/10.1016/j.xpro.2023.102698
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