Protocol for preparing Thiopropyl Sepharose resin used for capturing S-nitrosylated proteins

S-nitrosothiol (SNO)-Resin Assisted Capture (SNO-RAC) relies on a Thiopropyl Sepharose resin to identify S-nitrosylated proteins (SNO-proteins) and sites of S-nitrosylation. Here, we present a protocol for preparing Thiopropyl Sepharose resin with efficiency of SNO-protein capture comparable to the...

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Detalles Bibliográficos
Autores principales: Seth, Puneet, Hausladen, Alfred, Premont, Richard T., Stamler, Jonathan S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10652206/
https://www.ncbi.nlm.nih.gov/pubmed/37925633
http://dx.doi.org/10.1016/j.xpro.2023.102430

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