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A single‐cell transcriptomic atlas characterizes age‐related changes of murine cranial stem cell niches

The craniofacial bones provide structural support for the skull and accommodate the vulnerable brain tissue with a protective cavity. The bone tissue undergoes constant turnover, which relies on skeletal stem cells (SSCs) and/or mesenchymal stem cells (MSCs) and their niches. SSCs/MSCs and their per...

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Detalles Bibliográficos
Autores principales: Li, Bo, Li, Jingya, Li, Bingzhi, Ouchi, Takehito, Li, Longjiang, Li, Yu, Zhao, Zhihe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10652347/
https://www.ncbi.nlm.nih.gov/pubmed/37681346
http://dx.doi.org/10.1111/acel.13980
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author Li, Bo
Li, Jingya
Li, Bingzhi
Ouchi, Takehito
Li, Longjiang
Li, Yu
Zhao, Zhihe
author_facet Li, Bo
Li, Jingya
Li, Bingzhi
Ouchi, Takehito
Li, Longjiang
Li, Yu
Zhao, Zhihe
author_sort Li, Bo
collection PubMed
description The craniofacial bones provide structural support for the skull and accommodate the vulnerable brain tissue with a protective cavity. The bone tissue undergoes constant turnover, which relies on skeletal stem cells (SSCs) and/or mesenchymal stem cells (MSCs) and their niches. SSCs/MSCs and their perivascular niche within the bone marrow are well characterized in long bones. As for cranial bones, besides bone marrow, the suture mesenchyme has been identified as a unique niche for SSCs/MSCs of craniofacial bones. However, a comprehensive study of the two different cranial stem cell niches at single‐cell resolution is still lacking. In addition, during the progression of aging, age‐associated changes in cranial stem cell niches and resident cells remain uncovered. In this study, we investigated age‐related changes in cranial stem cell niches via single‐cell RNA sequencing (scRNA‐seq). The transcriptomic profiles and cellular compositions have been delineated, indicating alterations of the cranial bone marrow microenvironment influenced by inflammaging. Moreover, we identified a senescent mesenchymal cell subcluster and several age‐related immune cell subclusters by reclustering and pseudotime trajectory analysis, which might be closely linked to inflammaging. Finally, differentially expressed genes (DEGs) and cell–cell communications were analyzed during aging, revealing potential regulatory factors. Overall, this work highlights the age‐related changes in cranial stem cell niches, which deepens the current understanding of cranial bone and suture biology and may provide therapeutic targets for antiaging and regenerative medicine.
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spelling pubmed-106523472023-09-08 A single‐cell transcriptomic atlas characterizes age‐related changes of murine cranial stem cell niches Li, Bo Li, Jingya Li, Bingzhi Ouchi, Takehito Li, Longjiang Li, Yu Zhao, Zhihe Aging Cell Research Articles The craniofacial bones provide structural support for the skull and accommodate the vulnerable brain tissue with a protective cavity. The bone tissue undergoes constant turnover, which relies on skeletal stem cells (SSCs) and/or mesenchymal stem cells (MSCs) and their niches. SSCs/MSCs and their perivascular niche within the bone marrow are well characterized in long bones. As for cranial bones, besides bone marrow, the suture mesenchyme has been identified as a unique niche for SSCs/MSCs of craniofacial bones. However, a comprehensive study of the two different cranial stem cell niches at single‐cell resolution is still lacking. In addition, during the progression of aging, age‐associated changes in cranial stem cell niches and resident cells remain uncovered. In this study, we investigated age‐related changes in cranial stem cell niches via single‐cell RNA sequencing (scRNA‐seq). The transcriptomic profiles and cellular compositions have been delineated, indicating alterations of the cranial bone marrow microenvironment influenced by inflammaging. Moreover, we identified a senescent mesenchymal cell subcluster and several age‐related immune cell subclusters by reclustering and pseudotime trajectory analysis, which might be closely linked to inflammaging. Finally, differentially expressed genes (DEGs) and cell–cell communications were analyzed during aging, revealing potential regulatory factors. Overall, this work highlights the age‐related changes in cranial stem cell niches, which deepens the current understanding of cranial bone and suture biology and may provide therapeutic targets for antiaging and regenerative medicine. John Wiley and Sons Inc. 2023-09-08 /pmc/articles/PMC10652347/ /pubmed/37681346 http://dx.doi.org/10.1111/acel.13980 Text en © 2023 The Authors. Aging Cell published by Anatomical Society and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Li, Bo
Li, Jingya
Li, Bingzhi
Ouchi, Takehito
Li, Longjiang
Li, Yu
Zhao, Zhihe
A single‐cell transcriptomic atlas characterizes age‐related changes of murine cranial stem cell niches
title A single‐cell transcriptomic atlas characterizes age‐related changes of murine cranial stem cell niches
title_full A single‐cell transcriptomic atlas characterizes age‐related changes of murine cranial stem cell niches
title_fullStr A single‐cell transcriptomic atlas characterizes age‐related changes of murine cranial stem cell niches
title_full_unstemmed A single‐cell transcriptomic atlas characterizes age‐related changes of murine cranial stem cell niches
title_short A single‐cell transcriptomic atlas characterizes age‐related changes of murine cranial stem cell niches
title_sort single‐cell transcriptomic atlas characterizes age‐related changes of murine cranial stem cell niches
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10652347/
https://www.ncbi.nlm.nih.gov/pubmed/37681346
http://dx.doi.org/10.1111/acel.13980
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