Cargando…

Characteristics of vaginal microbiota in various cervical intraepithelial neoplasia: a cross-sectional study

BACKGROUND: Precancerous lesions of cervical cancer exhibit characteristics indicative of natural progression. To prevent overtreatment of patients whose cervical intraepithelial neoplasia (CIN) in regression and to predict the onset of invasive cervical cancer at an early stage, we've identifi...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Yiran, Wang, Shuzhen, Liu, Jun, Su, Mingrui, Diao, Xiaoli, Liang, Xiaolong, Zhang, Jianxin, Wang, Qiuxi, Zhan, Yuxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10652498/
https://www.ncbi.nlm.nih.gov/pubmed/37974192
http://dx.doi.org/10.1186/s12967-023-04676-5
_version_ 1785147697641029632
author Liu, Yiran
Wang, Shuzhen
Liu, Jun
Su, Mingrui
Diao, Xiaoli
Liang, Xiaolong
Zhang, Jianxin
Wang, Qiuxi
Zhan, Yuxin
author_facet Liu, Yiran
Wang, Shuzhen
Liu, Jun
Su, Mingrui
Diao, Xiaoli
Liang, Xiaolong
Zhang, Jianxin
Wang, Qiuxi
Zhan, Yuxin
author_sort Liu, Yiran
collection PubMed
description BACKGROUND: Precancerous lesions of cervical cancer exhibit characteristics indicative of natural progression. To prevent overtreatment of patients whose cervical intraepithelial neoplasia (CIN) in regression and to predict the onset of invasive cervical cancer at an early stage, we've identified the vaginal microbiome as a potential key factor, which is associated with both HPV infection and the various cervical intraepithelial neoplasia. This study aims to investigate the microbiome characteristics of patients with various cervical intraepithelial neoplasia. METHODS: Utilizing high-throughput 16S ribosomal RNA (16S rRNA) sequencing technology, a description of the characteristics and community composition of Vaginal Microbiota (VMB) was conducted among 692 Chinese women infected with the High-risk Human Papillomavirus (HR-HPV). RESULTS: As the grade of the lesions increased, the proportions of Lactobacillus and Pseudomonas demonstrated a significant declining trend, while the proportions of Gardnerella, Dialister, and Prevotella significantly increased. The diversity of the VMB was more significant in high-grade CIN. Furthermore, KEGG pathway enrichment analysis indicates that high-grade cervical intraepithelial neoplasia can inhibit various pathways, including those of phosphotransferase system, transcription factors, Fructose and mannose metabolism, amino sugar and nucleotide sugar metabolism, and galactose metabolism, which may contribute to the development of early cervical cancer symptoms. CONCLUSION: Patients with CIN exhibit a distinct vaginal microbial profile characterized by a decrease in Lactobacillus and Pseudomonas, and an increase in Gardnerella, Prevotella, and Dialister. The proliferation and diminution of these two types of microbial communities are interrelated, suggesting a mutual restraint and balance among them. Disruption of this regulatory balance could potentially lead to the onset of cervical lesions and carcinogenesis. Retrospectively registered: This study was approved by the Ethics Committee of the Beijing Chaoyang Hospital affiliated with the Capital Medical University (NO.2023-S-415).
format Online
Article
Text
id pubmed-10652498
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-106524982023-11-16 Characteristics of vaginal microbiota in various cervical intraepithelial neoplasia: a cross-sectional study Liu, Yiran Wang, Shuzhen Liu, Jun Su, Mingrui Diao, Xiaoli Liang, Xiaolong Zhang, Jianxin Wang, Qiuxi Zhan, Yuxin J Transl Med Research BACKGROUND: Precancerous lesions of cervical cancer exhibit characteristics indicative of natural progression. To prevent overtreatment of patients whose cervical intraepithelial neoplasia (CIN) in regression and to predict the onset of invasive cervical cancer at an early stage, we've identified the vaginal microbiome as a potential key factor, which is associated with both HPV infection and the various cervical intraepithelial neoplasia. This study aims to investigate the microbiome characteristics of patients with various cervical intraepithelial neoplasia. METHODS: Utilizing high-throughput 16S ribosomal RNA (16S rRNA) sequencing technology, a description of the characteristics and community composition of Vaginal Microbiota (VMB) was conducted among 692 Chinese women infected with the High-risk Human Papillomavirus (HR-HPV). RESULTS: As the grade of the lesions increased, the proportions of Lactobacillus and Pseudomonas demonstrated a significant declining trend, while the proportions of Gardnerella, Dialister, and Prevotella significantly increased. The diversity of the VMB was more significant in high-grade CIN. Furthermore, KEGG pathway enrichment analysis indicates that high-grade cervical intraepithelial neoplasia can inhibit various pathways, including those of phosphotransferase system, transcription factors, Fructose and mannose metabolism, amino sugar and nucleotide sugar metabolism, and galactose metabolism, which may contribute to the development of early cervical cancer symptoms. CONCLUSION: Patients with CIN exhibit a distinct vaginal microbial profile characterized by a decrease in Lactobacillus and Pseudomonas, and an increase in Gardnerella, Prevotella, and Dialister. The proliferation and diminution of these two types of microbial communities are interrelated, suggesting a mutual restraint and balance among them. Disruption of this regulatory balance could potentially lead to the onset of cervical lesions and carcinogenesis. Retrospectively registered: This study was approved by the Ethics Committee of the Beijing Chaoyang Hospital affiliated with the Capital Medical University (NO.2023-S-415). BioMed Central 2023-11-16 /pmc/articles/PMC10652498/ /pubmed/37974192 http://dx.doi.org/10.1186/s12967-023-04676-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Liu, Yiran
Wang, Shuzhen
Liu, Jun
Su, Mingrui
Diao, Xiaoli
Liang, Xiaolong
Zhang, Jianxin
Wang, Qiuxi
Zhan, Yuxin
Characteristics of vaginal microbiota in various cervical intraepithelial neoplasia: a cross-sectional study
title Characteristics of vaginal microbiota in various cervical intraepithelial neoplasia: a cross-sectional study
title_full Characteristics of vaginal microbiota in various cervical intraepithelial neoplasia: a cross-sectional study
title_fullStr Characteristics of vaginal microbiota in various cervical intraepithelial neoplasia: a cross-sectional study
title_full_unstemmed Characteristics of vaginal microbiota in various cervical intraepithelial neoplasia: a cross-sectional study
title_short Characteristics of vaginal microbiota in various cervical intraepithelial neoplasia: a cross-sectional study
title_sort characteristics of vaginal microbiota in various cervical intraepithelial neoplasia: a cross-sectional study
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10652498/
https://www.ncbi.nlm.nih.gov/pubmed/37974192
http://dx.doi.org/10.1186/s12967-023-04676-5
work_keys_str_mv AT liuyiran characteristicsofvaginalmicrobiotainvariouscervicalintraepithelialneoplasiaacrosssectionalstudy
AT wangshuzhen characteristicsofvaginalmicrobiotainvariouscervicalintraepithelialneoplasiaacrosssectionalstudy
AT liujun characteristicsofvaginalmicrobiotainvariouscervicalintraepithelialneoplasiaacrosssectionalstudy
AT sumingrui characteristicsofvaginalmicrobiotainvariouscervicalintraepithelialneoplasiaacrosssectionalstudy
AT diaoxiaoli characteristicsofvaginalmicrobiotainvariouscervicalintraepithelialneoplasiaacrosssectionalstudy
AT liangxiaolong characteristicsofvaginalmicrobiotainvariouscervicalintraepithelialneoplasiaacrosssectionalstudy
AT zhangjianxin characteristicsofvaginalmicrobiotainvariouscervicalintraepithelialneoplasiaacrosssectionalstudy
AT wangqiuxi characteristicsofvaginalmicrobiotainvariouscervicalintraepithelialneoplasiaacrosssectionalstudy
AT zhanyuxin characteristicsofvaginalmicrobiotainvariouscervicalintraepithelialneoplasiaacrosssectionalstudy