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Transcriptome analysis of Artemisia argyi following methyl jasmonate (MeJA) treatment and the mining of genes related to the stress resistance pathway

Artemisia argyi Lev. et Vant. (A. argyi) is a perennial grass in the Artemisia family, the plant has a strong aroma. Methyl jasmonate (MeJA) is critical to plant growth and development, stress response, and secondary metabolic processes. The experimental material Artemisia argyi was utilized in this...

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Autores principales: Wang, Jing, Cui, Yupeng, Li, Shuyan, Gao, Xinqiang, Zhang, Kunpeng, Shen, Xiangling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10652873/
https://www.ncbi.nlm.nih.gov/pubmed/38028600
http://dx.doi.org/10.3389/fgene.2023.1279850
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author Wang, Jing
Cui, Yupeng
Li, Shuyan
Gao, Xinqiang
Zhang, Kunpeng
Shen, Xiangling
author_facet Wang, Jing
Cui, Yupeng
Li, Shuyan
Gao, Xinqiang
Zhang, Kunpeng
Shen, Xiangling
author_sort Wang, Jing
collection PubMed
description Artemisia argyi Lev. et Vant. (A. argyi) is a perennial grass in the Artemisia family, the plant has a strong aroma. Methyl jasmonate (MeJA) is critical to plant growth and development, stress response, and secondary metabolic processes. The experimental material Artemisia argyi was utilized in this study to investigate the treatment of A. argyi with exogenous MeJA at concentrations of 100 and 200 μmol/L for durations of 9 and 24 h respectively. Transcriptome sequencing was conducted using the Illumina HiSeq platform to identify stress resistance-related candidate genes. Finally, a total of 102.43 Gb of data were obtained and 162,272 unigenes were identified. Differential analysis before and after MeJA treatment resulted in the screening of 20,776 differentially expressed genes. The GO classification revealed that the annotated unigenes were categorized into three distinct groups: cellular component, molecular function, and biological process. Notably, binding, metabolic process, and cellular process emerged as the most prevalent categories among them. The results of KEGG pathway statistical analysis revealed that plant hormone signal transduction, MAPK signaling pathway-plant, and plant-pathogen interaction were significant transduction pathways in A. argyi’s response to exogenous MeJA-induced abiotic stress. With the alteration of exogenous MeJA concentration and duration, a significant upregulation was observed in the expression levels of calmodulin CaM4 (ID: EVM0136224) involved in MAPK signaling pathway-plant and auxin response factor ARF (ID: EVM0055178) associated with plant-pathogen interaction. The findings of this study establish a solid theoretical foundation for the future development of highly resistant varieties of A. argyi.
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spelling pubmed-106528732023-11-02 Transcriptome analysis of Artemisia argyi following methyl jasmonate (MeJA) treatment and the mining of genes related to the stress resistance pathway Wang, Jing Cui, Yupeng Li, Shuyan Gao, Xinqiang Zhang, Kunpeng Shen, Xiangling Front Genet Genetics Artemisia argyi Lev. et Vant. (A. argyi) is a perennial grass in the Artemisia family, the plant has a strong aroma. Methyl jasmonate (MeJA) is critical to plant growth and development, stress response, and secondary metabolic processes. The experimental material Artemisia argyi was utilized in this study to investigate the treatment of A. argyi with exogenous MeJA at concentrations of 100 and 200 μmol/L for durations of 9 and 24 h respectively. Transcriptome sequencing was conducted using the Illumina HiSeq platform to identify stress resistance-related candidate genes. Finally, a total of 102.43 Gb of data were obtained and 162,272 unigenes were identified. Differential analysis before and after MeJA treatment resulted in the screening of 20,776 differentially expressed genes. The GO classification revealed that the annotated unigenes were categorized into three distinct groups: cellular component, molecular function, and biological process. Notably, binding, metabolic process, and cellular process emerged as the most prevalent categories among them. The results of KEGG pathway statistical analysis revealed that plant hormone signal transduction, MAPK signaling pathway-plant, and plant-pathogen interaction were significant transduction pathways in A. argyi’s response to exogenous MeJA-induced abiotic stress. With the alteration of exogenous MeJA concentration and duration, a significant upregulation was observed in the expression levels of calmodulin CaM4 (ID: EVM0136224) involved in MAPK signaling pathway-plant and auxin response factor ARF (ID: EVM0055178) associated with plant-pathogen interaction. The findings of this study establish a solid theoretical foundation for the future development of highly resistant varieties of A. argyi. Frontiers Media S.A. 2023-11-02 /pmc/articles/PMC10652873/ /pubmed/38028600 http://dx.doi.org/10.3389/fgene.2023.1279850 Text en Copyright © 2023 Wang, Cui, Li, Gao, Zhang and Shen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Wang, Jing
Cui, Yupeng
Li, Shuyan
Gao, Xinqiang
Zhang, Kunpeng
Shen, Xiangling
Transcriptome analysis of Artemisia argyi following methyl jasmonate (MeJA) treatment and the mining of genes related to the stress resistance pathway
title Transcriptome analysis of Artemisia argyi following methyl jasmonate (MeJA) treatment and the mining of genes related to the stress resistance pathway
title_full Transcriptome analysis of Artemisia argyi following methyl jasmonate (MeJA) treatment and the mining of genes related to the stress resistance pathway
title_fullStr Transcriptome analysis of Artemisia argyi following methyl jasmonate (MeJA) treatment and the mining of genes related to the stress resistance pathway
title_full_unstemmed Transcriptome analysis of Artemisia argyi following methyl jasmonate (MeJA) treatment and the mining of genes related to the stress resistance pathway
title_short Transcriptome analysis of Artemisia argyi following methyl jasmonate (MeJA) treatment and the mining of genes related to the stress resistance pathway
title_sort transcriptome analysis of artemisia argyi following methyl jasmonate (meja) treatment and the mining of genes related to the stress resistance pathway
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10652873/
https://www.ncbi.nlm.nih.gov/pubmed/38028600
http://dx.doi.org/10.3389/fgene.2023.1279850
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