Cargando…

Arthritis imaging using a near-infrared fluorescence folate-targeted probe

A recently developed near-infrared fluorescence-labeled folate probe (NIR2-folate) was tested for in vivo imaging of arthritis using a lipopolysaccharide intra-articular injection model and a KRN transgenic mice serum induction mouse model. In the lipopolysaccharide injection model, the fluorescence...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Wei-Tsung, Mahmood, Umar, Weissleder, Ralph, Tung, Ching-Hsuan
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1065321/
https://www.ncbi.nlm.nih.gov/pubmed/15743478
http://dx.doi.org/10.1186/ar1483
_version_ 1782123363404087296
author Chen, Wei-Tsung
Mahmood, Umar
Weissleder, Ralph
Tung, Ching-Hsuan
author_facet Chen, Wei-Tsung
Mahmood, Umar
Weissleder, Ralph
Tung, Ching-Hsuan
author_sort Chen, Wei-Tsung
collection PubMed
description A recently developed near-infrared fluorescence-labeled folate probe (NIR2-folate) was tested for in vivo imaging of arthritis using a lipopolysaccharide intra-articular injection model and a KRN transgenic mice serum induction mouse model. In the lipopolysaccharide injection model, the fluorescence signal intensity of NIR2-folate (n = 12) and of free NIR2 (n = 5) was compared between lipopolysaccharide-treated and control joints. The fluorescence signal intensity of the NIR2-folate probe at the inflammatory joints was found to be significantly higher than the control normal joints (up to 2.3-fold, P < 0.001). The NIR2-free dye injection group showed a persistent lower enhancement ratio than the NIR2-folate probe injection group. Excessive folic acid was also given to demonstrate a competitive effect with the NIR2-folate. In the KRN serum transfer model (n = 4), NIR2-folate was applied at different time points after serum transfer, and the inflamed joints could be detected as early as 30 hours after arthritogenic antibody transfer (1.8-fold increase in signal intensity). Fluorescence microscopy, histology, and immunohistochemistry validated the optical imaging results. We conclude that in vivo arthritis detection was feasible using a folate-targeted near-infrared fluorescence probe. This receptor-targeted imaging method may facilitate improved arthritis diagnosis and early assessment of the disease progress by providing an in vivo characterization of active macrophage status in inflammatory joint diseases.
format Text
id pubmed-1065321
institution National Center for Biotechnology Information
language English
publishDate 2005
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-10653212005-03-16 Arthritis imaging using a near-infrared fluorescence folate-targeted probe Chen, Wei-Tsung Mahmood, Umar Weissleder, Ralph Tung, Ching-Hsuan Arthritis Res Ther Research Article A recently developed near-infrared fluorescence-labeled folate probe (NIR2-folate) was tested for in vivo imaging of arthritis using a lipopolysaccharide intra-articular injection model and a KRN transgenic mice serum induction mouse model. In the lipopolysaccharide injection model, the fluorescence signal intensity of NIR2-folate (n = 12) and of free NIR2 (n = 5) was compared between lipopolysaccharide-treated and control joints. The fluorescence signal intensity of the NIR2-folate probe at the inflammatory joints was found to be significantly higher than the control normal joints (up to 2.3-fold, P < 0.001). The NIR2-free dye injection group showed a persistent lower enhancement ratio than the NIR2-folate probe injection group. Excessive folic acid was also given to demonstrate a competitive effect with the NIR2-folate. In the KRN serum transfer model (n = 4), NIR2-folate was applied at different time points after serum transfer, and the inflamed joints could be detected as early as 30 hours after arthritogenic antibody transfer (1.8-fold increase in signal intensity). Fluorescence microscopy, histology, and immunohistochemistry validated the optical imaging results. We conclude that in vivo arthritis detection was feasible using a folate-targeted near-infrared fluorescence probe. This receptor-targeted imaging method may facilitate improved arthritis diagnosis and early assessment of the disease progress by providing an in vivo characterization of active macrophage status in inflammatory joint diseases. BioMed Central 2005 2005-01-14 /pmc/articles/PMC1065321/ /pubmed/15743478 http://dx.doi.org/10.1186/ar1483 Text en Copyright © 2005 Chen et al., licensee BioMed Central Ltd.
spellingShingle Research Article
Chen, Wei-Tsung
Mahmood, Umar
Weissleder, Ralph
Tung, Ching-Hsuan
Arthritis imaging using a near-infrared fluorescence folate-targeted probe
title Arthritis imaging using a near-infrared fluorescence folate-targeted probe
title_full Arthritis imaging using a near-infrared fluorescence folate-targeted probe
title_fullStr Arthritis imaging using a near-infrared fluorescence folate-targeted probe
title_full_unstemmed Arthritis imaging using a near-infrared fluorescence folate-targeted probe
title_short Arthritis imaging using a near-infrared fluorescence folate-targeted probe
title_sort arthritis imaging using a near-infrared fluorescence folate-targeted probe
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1065321/
https://www.ncbi.nlm.nih.gov/pubmed/15743478
http://dx.doi.org/10.1186/ar1483
work_keys_str_mv AT chenweitsung arthritisimagingusinganearinfraredfluorescencefolatetargetedprobe
AT mahmoodumar arthritisimagingusinganearinfraredfluorescencefolatetargetedprobe
AT weisslederralph arthritisimagingusinganearinfraredfluorescencefolatetargetedprobe
AT tungchinghsuan arthritisimagingusinganearinfraredfluorescencefolatetargetedprobe