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A novel candidate hepatitis C virus genotype 4 subtype identified by next generation sequencing full-genome characterization in a patient from Saudi Arabia

BACKGROUND AND AIM: Hepatitis C virus (HCV) infection is a major global public health concern, being a leading cause of chronic liver diseases such as chronic hepatitis, cirrhosis, and hepatocellular carcinoma. The virus is classified into 8 genotypes and 93 subtypes, each displaying distinct geogra...

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Autores principales: Di Stefano, Mariantonietta, Ismail, Mona H., Leitner, Thomas, Faleo, Giuseppina, Alwazzeh, Marwan Jabr, Mbisa, Jean Lutamyo, Fiore, Josè Ramon, Santantonio, Teresa Antonia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10653324/
https://www.ncbi.nlm.nih.gov/pubmed/38029191
http://dx.doi.org/10.3389/fmicb.2023.1285367
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author Di Stefano, Mariantonietta
Ismail, Mona H.
Leitner, Thomas
Faleo, Giuseppina
Alwazzeh, Marwan Jabr
Mbisa, Jean Lutamyo
Fiore, Josè Ramon
Santantonio, Teresa Antonia
author_facet Di Stefano, Mariantonietta
Ismail, Mona H.
Leitner, Thomas
Faleo, Giuseppina
Alwazzeh, Marwan Jabr
Mbisa, Jean Lutamyo
Fiore, Josè Ramon
Santantonio, Teresa Antonia
author_sort Di Stefano, Mariantonietta
collection PubMed
description BACKGROUND AND AIM: Hepatitis C virus (HCV) infection is a major global public health concern, being a leading cause of chronic liver diseases such as chronic hepatitis, cirrhosis, and hepatocellular carcinoma. The virus is classified into 8 genotypes and 93 subtypes, each displaying distinct geographic distributions. Genotype 4 is the most predominant in the Middle East and Eastern Mediterranean and is associated with high rates of hepatitis C infection worldwide. This study used next-generation sequencing to fully characterize the HCV genome and identify a novel subtype within genotype 4 isolated from a 64-year-old Saudi man diagnosed with hepatitis C. METHODS: We analyzed the complete genome of the 141-HCV isolate using whole-genome sequencing. RESULTS: Our phylogenetic reconstructions, based on the entire genome of HCV-4 strains, revealed that the 141-HCV isolate formed a distinct group within the genotype 4 classification, providing valuable new insights into the variability of HCV. CONCLUSION: This discovery of a previously unclassified HCV subtype within genotype 4 sheds light on the ongoing evolution and diversity of the virus. Such knowledge has significant implications for diagnostic and therapeutic approaches, as different subtypes may exhibit varying drug sensitivities and resistance profiles.
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spelling pubmed-106533242023-11-02 A novel candidate hepatitis C virus genotype 4 subtype identified by next generation sequencing full-genome characterization in a patient from Saudi Arabia Di Stefano, Mariantonietta Ismail, Mona H. Leitner, Thomas Faleo, Giuseppina Alwazzeh, Marwan Jabr Mbisa, Jean Lutamyo Fiore, Josè Ramon Santantonio, Teresa Antonia Front Microbiol Microbiology BACKGROUND AND AIM: Hepatitis C virus (HCV) infection is a major global public health concern, being a leading cause of chronic liver diseases such as chronic hepatitis, cirrhosis, and hepatocellular carcinoma. The virus is classified into 8 genotypes and 93 subtypes, each displaying distinct geographic distributions. Genotype 4 is the most predominant in the Middle East and Eastern Mediterranean and is associated with high rates of hepatitis C infection worldwide. This study used next-generation sequencing to fully characterize the HCV genome and identify a novel subtype within genotype 4 isolated from a 64-year-old Saudi man diagnosed with hepatitis C. METHODS: We analyzed the complete genome of the 141-HCV isolate using whole-genome sequencing. RESULTS: Our phylogenetic reconstructions, based on the entire genome of HCV-4 strains, revealed that the 141-HCV isolate formed a distinct group within the genotype 4 classification, providing valuable new insights into the variability of HCV. CONCLUSION: This discovery of a previously unclassified HCV subtype within genotype 4 sheds light on the ongoing evolution and diversity of the virus. Such knowledge has significant implications for diagnostic and therapeutic approaches, as different subtypes may exhibit varying drug sensitivities and resistance profiles. Frontiers Media S.A. 2023-11-02 /pmc/articles/PMC10653324/ /pubmed/38029191 http://dx.doi.org/10.3389/fmicb.2023.1285367 Text en Copyright © 2023 Di Stefano, Ismail, Leitner, Faleo, Alwazzeh, Mbisa, Fiore and Santantonio. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Di Stefano, Mariantonietta
Ismail, Mona H.
Leitner, Thomas
Faleo, Giuseppina
Alwazzeh, Marwan Jabr
Mbisa, Jean Lutamyo
Fiore, Josè Ramon
Santantonio, Teresa Antonia
A novel candidate hepatitis C virus genotype 4 subtype identified by next generation sequencing full-genome characterization in a patient from Saudi Arabia
title A novel candidate hepatitis C virus genotype 4 subtype identified by next generation sequencing full-genome characterization in a patient from Saudi Arabia
title_full A novel candidate hepatitis C virus genotype 4 subtype identified by next generation sequencing full-genome characterization in a patient from Saudi Arabia
title_fullStr A novel candidate hepatitis C virus genotype 4 subtype identified by next generation sequencing full-genome characterization in a patient from Saudi Arabia
title_full_unstemmed A novel candidate hepatitis C virus genotype 4 subtype identified by next generation sequencing full-genome characterization in a patient from Saudi Arabia
title_short A novel candidate hepatitis C virus genotype 4 subtype identified by next generation sequencing full-genome characterization in a patient from Saudi Arabia
title_sort novel candidate hepatitis c virus genotype 4 subtype identified by next generation sequencing full-genome characterization in a patient from saudi arabia
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10653324/
https://www.ncbi.nlm.nih.gov/pubmed/38029191
http://dx.doi.org/10.3389/fmicb.2023.1285367
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