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Expression of ZNF281 in colorectal cancer correlates with response to radiotherapy and survival
BACKGROUND: The treatment of Colorectal cancer (CRC) is extremely complex and survival rates vary depending on the stage of the disease at the time of diagnosis. Neoadjuvant chemoradiotherapy (NACRT), is the conventional treatment for locally advanced rectal cancer (LARC); however, the resistance to...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10653697/ https://www.ncbi.nlm.nih.gov/pubmed/37939252 http://dx.doi.org/10.1080/07853890.2023.2278619 |
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author | Qin, Changjiang Li, Ang Xiao, Yafei Liu, Wenjing Zhai, Ertao Li, Quanying Jing, Hong Zhang, Yijie Zhang, Hui Ma, Xuhui Tang, Hongna Rong, Dan |
author_facet | Qin, Changjiang Li, Ang Xiao, Yafei Liu, Wenjing Zhai, Ertao Li, Quanying Jing, Hong Zhang, Yijie Zhang, Hui Ma, Xuhui Tang, Hongna Rong, Dan |
author_sort | Qin, Changjiang |
collection | PubMed |
description | BACKGROUND: The treatment of Colorectal cancer (CRC) is extremely complex and survival rates vary depending on the stage of the disease at the time of diagnosis. Neoadjuvant chemoradiotherapy (NACRT), is the conventional treatment for locally advanced rectal cancer (LARC); however, the resistance to chemoradiotherapy in LARC is difficult to predict. MATERIALS AND METHODS: In this study, clinical data of 126 LARC patients were collected and analyzed, and relevant validation was performed using GEO database and in vitro and in vivo experiments, including Western blotting and Real-time quantitative PCR, immunohistochemistry, immunofluorescence, clonogenic cell survival assays, and nude-mouse xenograft models. RESULTS: In patients with LARC who were treated with neoadjuvant radiotherapy (NART), higher ZNF281 expression in malignant tissue was associated with a poorer prognosis and lesser degree of tumor regression. Cell and mouse experiments have shown that ZNF281 reduces the damage caused by X-rays to CRC cells and tumors grown in mice. CONCLUSION: We found that the expression of ZNF281 predicted the radiation response of CRC cells and suggested the prognosis of patients with LARC who received neoadjuvant radiation therapy. |
format | Online Article Text |
id | pubmed-10653697 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-106536972023-11-08 Expression of ZNF281 in colorectal cancer correlates with response to radiotherapy and survival Qin, Changjiang Li, Ang Xiao, Yafei Liu, Wenjing Zhai, Ertao Li, Quanying Jing, Hong Zhang, Yijie Zhang, Hui Ma, Xuhui Tang, Hongna Rong, Dan Ann Med Oncology BACKGROUND: The treatment of Colorectal cancer (CRC) is extremely complex and survival rates vary depending on the stage of the disease at the time of diagnosis. Neoadjuvant chemoradiotherapy (NACRT), is the conventional treatment for locally advanced rectal cancer (LARC); however, the resistance to chemoradiotherapy in LARC is difficult to predict. MATERIALS AND METHODS: In this study, clinical data of 126 LARC patients were collected and analyzed, and relevant validation was performed using GEO database and in vitro and in vivo experiments, including Western blotting and Real-time quantitative PCR, immunohistochemistry, immunofluorescence, clonogenic cell survival assays, and nude-mouse xenograft models. RESULTS: In patients with LARC who were treated with neoadjuvant radiotherapy (NART), higher ZNF281 expression in malignant tissue was associated with a poorer prognosis and lesser degree of tumor regression. Cell and mouse experiments have shown that ZNF281 reduces the damage caused by X-rays to CRC cells and tumors grown in mice. CONCLUSION: We found that the expression of ZNF281 predicted the radiation response of CRC cells and suggested the prognosis of patients with LARC who received neoadjuvant radiation therapy. Taylor & Francis 2023-11-08 /pmc/articles/PMC10653697/ /pubmed/37939252 http://dx.doi.org/10.1080/07853890.2023.2278619 Text en © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent. |
spellingShingle | Oncology Qin, Changjiang Li, Ang Xiao, Yafei Liu, Wenjing Zhai, Ertao Li, Quanying Jing, Hong Zhang, Yijie Zhang, Hui Ma, Xuhui Tang, Hongna Rong, Dan Expression of ZNF281 in colorectal cancer correlates with response to radiotherapy and survival |
title | Expression of ZNF281 in colorectal cancer correlates with response to radiotherapy and survival |
title_full | Expression of ZNF281 in colorectal cancer correlates with response to radiotherapy and survival |
title_fullStr | Expression of ZNF281 in colorectal cancer correlates with response to radiotherapy and survival |
title_full_unstemmed | Expression of ZNF281 in colorectal cancer correlates with response to radiotherapy and survival |
title_short | Expression of ZNF281 in colorectal cancer correlates with response to radiotherapy and survival |
title_sort | expression of znf281 in colorectal cancer correlates with response to radiotherapy and survival |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10653697/ https://www.ncbi.nlm.nih.gov/pubmed/37939252 http://dx.doi.org/10.1080/07853890.2023.2278619 |
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