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Synechococcus elongatus Argonaute reduces natural transformation efficiency and provides immunity against exogenous plasmids

The cyanobacterium Synechococcus elongatus PCC 7942 produces an active prokaryotic Argonaute nuclease, SeAgo, whose function is unknown. Here, we show that SeAgo reduces natural transformation and prevents the maintenance of RSF1010 replicons in S. elongatus. In addition, a Cas4-like nuclease and tw...

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Autores principales: Taton, Arnaud, Gilderman, Tami S., Ernst, Dustin C., Omaga, Carla A., Cohen, Lucas A., Rey-Bedon, Camilo, Golden, James W., Golden, Susan S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10653904/
https://www.ncbi.nlm.nih.gov/pubmed/37791787
http://dx.doi.org/10.1128/mbio.01843-23
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author Taton, Arnaud
Gilderman, Tami S.
Ernst, Dustin C.
Omaga, Carla A.
Cohen, Lucas A.
Rey-Bedon, Camilo
Golden, James W.
Golden, Susan S.
author_facet Taton, Arnaud
Gilderman, Tami S.
Ernst, Dustin C.
Omaga, Carla A.
Cohen, Lucas A.
Rey-Bedon, Camilo
Golden, James W.
Golden, Susan S.
author_sort Taton, Arnaud
collection PubMed
description The cyanobacterium Synechococcus elongatus PCC 7942 produces an active prokaryotic Argonaute nuclease, SeAgo, whose function is unknown. Here, we show that SeAgo reduces natural transformation and prevents the maintenance of RSF1010 replicons in S. elongatus. In addition, a Cas4-like nuclease and two other proteins, UvrD and RecJ(cy) (cyanobacterial lineage), were found to reduce the transfer or maintenance of RSF1010 replicons. Like other prokaryotic Argonautes, our results indicate that SeAgo provides defense against invading DNA. An S. elongatus ago deletion strain shares the same morphology, growth rate, and circadian gene expression as the wild type, has higher transformation efficiency, and enables the use of RSF1010-based plasmids for genetic engineering. IMPORTANCE: S. elongatus is an important cyanobacterial model organism for the study of its prokaryotic circadian clock, photosynthesis, and other biological processes. It is also widely used for genetic engineering to produce renewable biochemicals. Our findings reveal an SeAgo-based defense mechanism in S. elongatus against the horizontal transfer of genetic material. We demonstrate that deletion of the ago gene facilitates genetic studies and genetic engineering of S. elongatus.
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spelling pubmed-106539042023-10-04 Synechococcus elongatus Argonaute reduces natural transformation efficiency and provides immunity against exogenous plasmids Taton, Arnaud Gilderman, Tami S. Ernst, Dustin C. Omaga, Carla A. Cohen, Lucas A. Rey-Bedon, Camilo Golden, James W. Golden, Susan S. mBio Research Article The cyanobacterium Synechococcus elongatus PCC 7942 produces an active prokaryotic Argonaute nuclease, SeAgo, whose function is unknown. Here, we show that SeAgo reduces natural transformation and prevents the maintenance of RSF1010 replicons in S. elongatus. In addition, a Cas4-like nuclease and two other proteins, UvrD and RecJ(cy) (cyanobacterial lineage), were found to reduce the transfer or maintenance of RSF1010 replicons. Like other prokaryotic Argonautes, our results indicate that SeAgo provides defense against invading DNA. An S. elongatus ago deletion strain shares the same morphology, growth rate, and circadian gene expression as the wild type, has higher transformation efficiency, and enables the use of RSF1010-based plasmids for genetic engineering. IMPORTANCE: S. elongatus is an important cyanobacterial model organism for the study of its prokaryotic circadian clock, photosynthesis, and other biological processes. It is also widely used for genetic engineering to produce renewable biochemicals. Our findings reveal an SeAgo-based defense mechanism in S. elongatus against the horizontal transfer of genetic material. We demonstrate that deletion of the ago gene facilitates genetic studies and genetic engineering of S. elongatus. American Society for Microbiology 2023-10-04 /pmc/articles/PMC10653904/ /pubmed/37791787 http://dx.doi.org/10.1128/mbio.01843-23 Text en Copyright © 2023 Taton et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Taton, Arnaud
Gilderman, Tami S.
Ernst, Dustin C.
Omaga, Carla A.
Cohen, Lucas A.
Rey-Bedon, Camilo
Golden, James W.
Golden, Susan S.
Synechococcus elongatus Argonaute reduces natural transformation efficiency and provides immunity against exogenous plasmids
title Synechococcus elongatus Argonaute reduces natural transformation efficiency and provides immunity against exogenous plasmids
title_full Synechococcus elongatus Argonaute reduces natural transformation efficiency and provides immunity against exogenous plasmids
title_fullStr Synechococcus elongatus Argonaute reduces natural transformation efficiency and provides immunity against exogenous plasmids
title_full_unstemmed Synechococcus elongatus Argonaute reduces natural transformation efficiency and provides immunity against exogenous plasmids
title_short Synechococcus elongatus Argonaute reduces natural transformation efficiency and provides immunity against exogenous plasmids
title_sort synechococcus elongatus argonaute reduces natural transformation efficiency and provides immunity against exogenous plasmids
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10653904/
https://www.ncbi.nlm.nih.gov/pubmed/37791787
http://dx.doi.org/10.1128/mbio.01843-23
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