Cargando…

Mutation in Polycomb repressive complex 2 gene OsFIE2 promotes asexual embryo formation in rice

Prevention of autonomous division of the egg apparatus and central cell in a female gametophyte before fertilization ensures successful reproduction in flowering plants. Here we show that rice ovules of Polycomb repressive complex 2 (PRC2) Osfie1 and Osfie2 double mutants exhibit asexual embryo and...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Xiaoba, Xie, Liqiong, Sun, Xizhe, Wang, Ningning, Finnegan, E. Jean, Helliwell, Chris, Yao, Jialing, Zhang, Hongyu, Wu, Xianjun, Hands, Phil, Lu, Falong, Ma, Lisong, Zhou, Bing, Chaudhury, Abed, Cao, Xiaofeng, Luo, Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10654051/
https://www.ncbi.nlm.nih.gov/pubmed/37814022
http://dx.doi.org/10.1038/s41477-023-01536-4
_version_ 1785136546563751936
author Wu, Xiaoba
Xie, Liqiong
Sun, Xizhe
Wang, Ningning
Finnegan, E. Jean
Helliwell, Chris
Yao, Jialing
Zhang, Hongyu
Wu, Xianjun
Hands, Phil
Lu, Falong
Ma, Lisong
Zhou, Bing
Chaudhury, Abed
Cao, Xiaofeng
Luo, Ming
author_facet Wu, Xiaoba
Xie, Liqiong
Sun, Xizhe
Wang, Ningning
Finnegan, E. Jean
Helliwell, Chris
Yao, Jialing
Zhang, Hongyu
Wu, Xianjun
Hands, Phil
Lu, Falong
Ma, Lisong
Zhou, Bing
Chaudhury, Abed
Cao, Xiaofeng
Luo, Ming
author_sort Wu, Xiaoba
collection PubMed
description Prevention of autonomous division of the egg apparatus and central cell in a female gametophyte before fertilization ensures successful reproduction in flowering plants. Here we show that rice ovules of Polycomb repressive complex 2 (PRC2) Osfie1 and Osfie2 double mutants exhibit asexual embryo and autonomous endosperm formation at a high frequency, while ovules of single Osfie2 mutants display asexual pre-embryo-like structures at a lower frequency without fertilization. Earlier onset, higher penetrance and better development of asexual embryos in the double mutants compared with those in Osfie2 suggest that the autonomous endosperm facilitated asexual embryo development. Transcriptomic analysis showed that male genome-expressed OsBBM1 and OsWOX8/9 were activated in the asexual embryos. Similarly, the maternal alleles of the paternally expressed imprinted genes were activated in the autonomous endosperm, suggesting that the egg apparatus and central cell convergently adopt PRC2 to maintain the non-dividing state before fertilization, possibly through silencing of the maternal alleles of male genome-expressed genes.
format Online
Article
Text
id pubmed-10654051
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-106540512023-10-09 Mutation in Polycomb repressive complex 2 gene OsFIE2 promotes asexual embryo formation in rice Wu, Xiaoba Xie, Liqiong Sun, Xizhe Wang, Ningning Finnegan, E. Jean Helliwell, Chris Yao, Jialing Zhang, Hongyu Wu, Xianjun Hands, Phil Lu, Falong Ma, Lisong Zhou, Bing Chaudhury, Abed Cao, Xiaofeng Luo, Ming Nat Plants Article Prevention of autonomous division of the egg apparatus and central cell in a female gametophyte before fertilization ensures successful reproduction in flowering plants. Here we show that rice ovules of Polycomb repressive complex 2 (PRC2) Osfie1 and Osfie2 double mutants exhibit asexual embryo and autonomous endosperm formation at a high frequency, while ovules of single Osfie2 mutants display asexual pre-embryo-like structures at a lower frequency without fertilization. Earlier onset, higher penetrance and better development of asexual embryos in the double mutants compared with those in Osfie2 suggest that the autonomous endosperm facilitated asexual embryo development. Transcriptomic analysis showed that male genome-expressed OsBBM1 and OsWOX8/9 were activated in the asexual embryos. Similarly, the maternal alleles of the paternally expressed imprinted genes were activated in the autonomous endosperm, suggesting that the egg apparatus and central cell convergently adopt PRC2 to maintain the non-dividing state before fertilization, possibly through silencing of the maternal alleles of male genome-expressed genes. Nature Publishing Group UK 2023-10-09 2023 /pmc/articles/PMC10654051/ /pubmed/37814022 http://dx.doi.org/10.1038/s41477-023-01536-4 Text en © Crown 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Wu, Xiaoba
Xie, Liqiong
Sun, Xizhe
Wang, Ningning
Finnegan, E. Jean
Helliwell, Chris
Yao, Jialing
Zhang, Hongyu
Wu, Xianjun
Hands, Phil
Lu, Falong
Ma, Lisong
Zhou, Bing
Chaudhury, Abed
Cao, Xiaofeng
Luo, Ming
Mutation in Polycomb repressive complex 2 gene OsFIE2 promotes asexual embryo formation in rice
title Mutation in Polycomb repressive complex 2 gene OsFIE2 promotes asexual embryo formation in rice
title_full Mutation in Polycomb repressive complex 2 gene OsFIE2 promotes asexual embryo formation in rice
title_fullStr Mutation in Polycomb repressive complex 2 gene OsFIE2 promotes asexual embryo formation in rice
title_full_unstemmed Mutation in Polycomb repressive complex 2 gene OsFIE2 promotes asexual embryo formation in rice
title_short Mutation in Polycomb repressive complex 2 gene OsFIE2 promotes asexual embryo formation in rice
title_sort mutation in polycomb repressive complex 2 gene osfie2 promotes asexual embryo formation in rice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10654051/
https://www.ncbi.nlm.nih.gov/pubmed/37814022
http://dx.doi.org/10.1038/s41477-023-01536-4
work_keys_str_mv AT wuxiaoba mutationinpolycombrepressivecomplex2geneosfie2promotesasexualembryoformationinrice
AT xieliqiong mutationinpolycombrepressivecomplex2geneosfie2promotesasexualembryoformationinrice
AT sunxizhe mutationinpolycombrepressivecomplex2geneosfie2promotesasexualembryoformationinrice
AT wangningning mutationinpolycombrepressivecomplex2geneosfie2promotesasexualembryoformationinrice
AT finneganejean mutationinpolycombrepressivecomplex2geneosfie2promotesasexualembryoformationinrice
AT helliwellchris mutationinpolycombrepressivecomplex2geneosfie2promotesasexualembryoformationinrice
AT yaojialing mutationinpolycombrepressivecomplex2geneosfie2promotesasexualembryoformationinrice
AT zhanghongyu mutationinpolycombrepressivecomplex2geneosfie2promotesasexualembryoformationinrice
AT wuxianjun mutationinpolycombrepressivecomplex2geneosfie2promotesasexualembryoformationinrice
AT handsphil mutationinpolycombrepressivecomplex2geneosfie2promotesasexualembryoformationinrice
AT lufalong mutationinpolycombrepressivecomplex2geneosfie2promotesasexualembryoformationinrice
AT malisong mutationinpolycombrepressivecomplex2geneosfie2promotesasexualembryoformationinrice
AT zhoubing mutationinpolycombrepressivecomplex2geneosfie2promotesasexualembryoformationinrice
AT chaudhuryabed mutationinpolycombrepressivecomplex2geneosfie2promotesasexualembryoformationinrice
AT caoxiaofeng mutationinpolycombrepressivecomplex2geneosfie2promotesasexualembryoformationinrice
AT luoming mutationinpolycombrepressivecomplex2geneosfie2promotesasexualembryoformationinrice