Cargando…

An oocyte meiotic midbody cap is required for developmental competence in mice

Embryo development depends upon maternally derived materials. Mammalian oocytes undergo extreme asymmetric cytokinesis events, producing one large egg and two small polar bodies. During cytokinesis in somatic cells, the midbody and subsequent assembly of the midbody remnant, a signaling organelle co...

Descripción completa

Detalles Bibliográficos
Autores principales: Jung, Gyu Ik, Londoño-Vásquez, Daniela, Park, Sungjin, Skop, Ahna R., Balboula, Ahmed Z., Schindler, Karen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10654508/
https://www.ncbi.nlm.nih.gov/pubmed/37973997
http://dx.doi.org/10.1038/s41467-023-43288-x
_version_ 1785147852961349632
author Jung, Gyu Ik
Londoño-Vásquez, Daniela
Park, Sungjin
Skop, Ahna R.
Balboula, Ahmed Z.
Schindler, Karen
author_facet Jung, Gyu Ik
Londoño-Vásquez, Daniela
Park, Sungjin
Skop, Ahna R.
Balboula, Ahmed Z.
Schindler, Karen
author_sort Jung, Gyu Ik
collection PubMed
description Embryo development depends upon maternally derived materials. Mammalian oocytes undergo extreme asymmetric cytokinesis events, producing one large egg and two small polar bodies. During cytokinesis in somatic cells, the midbody and subsequent assembly of the midbody remnant, a signaling organelle containing RNAs, transcription factors and translation machinery, is thought to influence cellular function or fate. The role of the midbody and midbody remnant in gametes, in particular, oocytes, remains unclear. Here, we examined the formation and function of meiotic midbodies (mMB) and mMB remnants using mouse oocytes and demonstrate that mMBs have a specialized cap structure that is orientated toward polar bodies. We show that that mMBs are translationally active, and that mMB caps are required to retain nascent proteins in eggs. We propose that this specialized mMB cap maintains genetic factors in eggs allowing for full developmental competency.
format Online
Article
Text
id pubmed-10654508
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-106545082023-11-16 An oocyte meiotic midbody cap is required for developmental competence in mice Jung, Gyu Ik Londoño-Vásquez, Daniela Park, Sungjin Skop, Ahna R. Balboula, Ahmed Z. Schindler, Karen Nat Commun Article Embryo development depends upon maternally derived materials. Mammalian oocytes undergo extreme asymmetric cytokinesis events, producing one large egg and two small polar bodies. During cytokinesis in somatic cells, the midbody and subsequent assembly of the midbody remnant, a signaling organelle containing RNAs, transcription factors and translation machinery, is thought to influence cellular function or fate. The role of the midbody and midbody remnant in gametes, in particular, oocytes, remains unclear. Here, we examined the formation and function of meiotic midbodies (mMB) and mMB remnants using mouse oocytes and demonstrate that mMBs have a specialized cap structure that is orientated toward polar bodies. We show that that mMBs are translationally active, and that mMB caps are required to retain nascent proteins in eggs. We propose that this specialized mMB cap maintains genetic factors in eggs allowing for full developmental competency. Nature Publishing Group UK 2023-11-16 /pmc/articles/PMC10654508/ /pubmed/37973997 http://dx.doi.org/10.1038/s41467-023-43288-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Jung, Gyu Ik
Londoño-Vásquez, Daniela
Park, Sungjin
Skop, Ahna R.
Balboula, Ahmed Z.
Schindler, Karen
An oocyte meiotic midbody cap is required for developmental competence in mice
title An oocyte meiotic midbody cap is required for developmental competence in mice
title_full An oocyte meiotic midbody cap is required for developmental competence in mice
title_fullStr An oocyte meiotic midbody cap is required for developmental competence in mice
title_full_unstemmed An oocyte meiotic midbody cap is required for developmental competence in mice
title_short An oocyte meiotic midbody cap is required for developmental competence in mice
title_sort oocyte meiotic midbody cap is required for developmental competence in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10654508/
https://www.ncbi.nlm.nih.gov/pubmed/37973997
http://dx.doi.org/10.1038/s41467-023-43288-x
work_keys_str_mv AT junggyuik anoocytemeioticmidbodycapisrequiredfordevelopmentalcompetenceinmice
AT londonovasquezdaniela anoocytemeioticmidbodycapisrequiredfordevelopmentalcompetenceinmice
AT parksungjin anoocytemeioticmidbodycapisrequiredfordevelopmentalcompetenceinmice
AT skopahnar anoocytemeioticmidbodycapisrequiredfordevelopmentalcompetenceinmice
AT balboulaahmedz anoocytemeioticmidbodycapisrequiredfordevelopmentalcompetenceinmice
AT schindlerkaren anoocytemeioticmidbodycapisrequiredfordevelopmentalcompetenceinmice
AT junggyuik oocytemeioticmidbodycapisrequiredfordevelopmentalcompetenceinmice
AT londonovasquezdaniela oocytemeioticmidbodycapisrequiredfordevelopmentalcompetenceinmice
AT parksungjin oocytemeioticmidbodycapisrequiredfordevelopmentalcompetenceinmice
AT skopahnar oocytemeioticmidbodycapisrequiredfordevelopmentalcompetenceinmice
AT balboulaahmedz oocytemeioticmidbodycapisrequiredfordevelopmentalcompetenceinmice
AT schindlerkaren oocytemeioticmidbodycapisrequiredfordevelopmentalcompetenceinmice