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Differential expression of immunoregulatory cytokines in adipose tissue and liver in response to high fat and high sugar diets in female mice

A comprehensive understanding of how dietary components impact immunoregulatory gene expression in adipose tissue (AT) and liver, and their respective contributions to metabolic health in mice, remains limited. The current study aimed to investigate the metabolic consequences of a high-sucrose diet...

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Autores principales: Weiner, Juliane, Dommel, Sebastian, Gebhardt, Claudia, Hanschkow, Martha, Popkova, Yulia, Krause, Kerstin, Klöting, Nora, Blüher, Matthias, Schiller, Jürgen, Heiker, John T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10655005/
https://www.ncbi.nlm.nih.gov/pubmed/38024380
http://dx.doi.org/10.3389/fnut.2023.1275160
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author Weiner, Juliane
Dommel, Sebastian
Gebhardt, Claudia
Hanschkow, Martha
Popkova, Yulia
Krause, Kerstin
Klöting, Nora
Blüher, Matthias
Schiller, Jürgen
Heiker, John T.
author_facet Weiner, Juliane
Dommel, Sebastian
Gebhardt, Claudia
Hanschkow, Martha
Popkova, Yulia
Krause, Kerstin
Klöting, Nora
Blüher, Matthias
Schiller, Jürgen
Heiker, John T.
author_sort Weiner, Juliane
collection PubMed
description A comprehensive understanding of how dietary components impact immunoregulatory gene expression in adipose tissue (AT) and liver, and their respective contributions to metabolic health in mice, remains limited. The current study aimed to investigate the metabolic consequences of a high-sucrose diet (HSD) and a high-fat diet (HFD) in female mice with a focus on differential lipid- and sucrose-induced changes in immunoregulatory gene expression in AT and liver. Female C57BL/6 J mice were fed a purified and macronutrient matched high fat, high sugar, or control diets for 12 weeks. Mice were extensively phenotyped, including glucose and insulin tolerance tests, adipose and liver gene and protein expression analysis by qPCR and Western blot, tissue lipid analyses, as well as histological analyses. Compared to the control diet, HSD- and HFD-fed mice had significantly higher body weights, with pronounced obesity along with glucose intolerance and insulin resistance only in HFD-fed mice. HSD-fed mice exhibited an intermediate phenotype, with mild metabolic deterioration at the end of the study. AT lipid composition was significantly altered by both diets, and inflammatory gene expression was only significantly induced in HFD-fed mice. In the liver however, histological analysis revealed that both HSD- and HFD-fed mice had pronounced ectopic lipid deposition indicating hepatic steatosis, but more pronounced in HSD-fed mice. This was in line with significant induction of pro-inflammatory gene expression specifically in livers of HSD-fed mice. Overall, our findings suggest that HFD consumption in female mice induces more profound inflammation in AT with pronounced deterioration of metabolic health, whereas HSD induced more pronounced hepatic steatosis and inflammation without yet affecting glucose metabolism.
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spelling pubmed-106550052023-01-01 Differential expression of immunoregulatory cytokines in adipose tissue and liver in response to high fat and high sugar diets in female mice Weiner, Juliane Dommel, Sebastian Gebhardt, Claudia Hanschkow, Martha Popkova, Yulia Krause, Kerstin Klöting, Nora Blüher, Matthias Schiller, Jürgen Heiker, John T. Front Nutr Nutrition A comprehensive understanding of how dietary components impact immunoregulatory gene expression in adipose tissue (AT) and liver, and their respective contributions to metabolic health in mice, remains limited. The current study aimed to investigate the metabolic consequences of a high-sucrose diet (HSD) and a high-fat diet (HFD) in female mice with a focus on differential lipid- and sucrose-induced changes in immunoregulatory gene expression in AT and liver. Female C57BL/6 J mice were fed a purified and macronutrient matched high fat, high sugar, or control diets for 12 weeks. Mice were extensively phenotyped, including glucose and insulin tolerance tests, adipose and liver gene and protein expression analysis by qPCR and Western blot, tissue lipid analyses, as well as histological analyses. Compared to the control diet, HSD- and HFD-fed mice had significantly higher body weights, with pronounced obesity along with glucose intolerance and insulin resistance only in HFD-fed mice. HSD-fed mice exhibited an intermediate phenotype, with mild metabolic deterioration at the end of the study. AT lipid composition was significantly altered by both diets, and inflammatory gene expression was only significantly induced in HFD-fed mice. In the liver however, histological analysis revealed that both HSD- and HFD-fed mice had pronounced ectopic lipid deposition indicating hepatic steatosis, but more pronounced in HSD-fed mice. This was in line with significant induction of pro-inflammatory gene expression specifically in livers of HSD-fed mice. Overall, our findings suggest that HFD consumption in female mice induces more profound inflammation in AT with pronounced deterioration of metabolic health, whereas HSD induced more pronounced hepatic steatosis and inflammation without yet affecting glucose metabolism. Frontiers Media S.A. 2023-11-03 /pmc/articles/PMC10655005/ /pubmed/38024380 http://dx.doi.org/10.3389/fnut.2023.1275160 Text en Copyright © 2023 Weiner, Dommel, Gebhardt, Hanschkow, Popkova, Krause, Klöting, Blüher, Schiller and Heiker. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Weiner, Juliane
Dommel, Sebastian
Gebhardt, Claudia
Hanschkow, Martha
Popkova, Yulia
Krause, Kerstin
Klöting, Nora
Blüher, Matthias
Schiller, Jürgen
Heiker, John T.
Differential expression of immunoregulatory cytokines in adipose tissue and liver in response to high fat and high sugar diets in female mice
title Differential expression of immunoregulatory cytokines in adipose tissue and liver in response to high fat and high sugar diets in female mice
title_full Differential expression of immunoregulatory cytokines in adipose tissue and liver in response to high fat and high sugar diets in female mice
title_fullStr Differential expression of immunoregulatory cytokines in adipose tissue and liver in response to high fat and high sugar diets in female mice
title_full_unstemmed Differential expression of immunoregulatory cytokines in adipose tissue and liver in response to high fat and high sugar diets in female mice
title_short Differential expression of immunoregulatory cytokines in adipose tissue and liver in response to high fat and high sugar diets in female mice
title_sort differential expression of immunoregulatory cytokines in adipose tissue and liver in response to high fat and high sugar diets in female mice
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10655005/
https://www.ncbi.nlm.nih.gov/pubmed/38024380
http://dx.doi.org/10.3389/fnut.2023.1275160
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