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Extracellular vesicles derived from different tissues attenuate cardiac dysfunction in murine MI models

BACKGROUND: Extracellular vesicles (EVs) derived from various cell sources exert cardioprotective effects during cardiac ischemic injury. Our previous study confirmed that EVs derived from ischemic-reperfusion injured heart tissue aggravated cardiac inflammation and dysfunction. However, the role of...

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Autores principales: Liu, Xuan, Shi, Shanshan, Geng, Xuedi, Wang, Enhao, Meng, Qingshu, Li, Mimi, Lin, Fang, Ma, Xiaoxue, Han, Wei, Zhou, Xiaohui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10655353/
https://www.ncbi.nlm.nih.gov/pubmed/37978390
http://dx.doi.org/10.1186/s13062-023-00429-y
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author Liu, Xuan
Shi, Shanshan
Geng, Xuedi
Wang, Enhao
Meng, Qingshu
Li, Mimi
Lin, Fang
Ma, Xiaoxue
Han, Wei
Zhou, Xiaohui
author_facet Liu, Xuan
Shi, Shanshan
Geng, Xuedi
Wang, Enhao
Meng, Qingshu
Li, Mimi
Lin, Fang
Ma, Xiaoxue
Han, Wei
Zhou, Xiaohui
author_sort Liu, Xuan
collection PubMed
description BACKGROUND: Extracellular vesicles (EVs) derived from various cell sources exert cardioprotective effects during cardiac ischemic injury. Our previous study confirmed that EVs derived from ischemic-reperfusion injured heart tissue aggravated cardiac inflammation and dysfunction. However, the role of EVs derived from normal cardiac tissue in myocardial ischemic injury remains elusive. RESULTS: In the present study, normal heart-derived EVs (cEVs) and kidney-derived EVs (nEVs) were isolated and intramyocardially injected into mice after myocardial infarction (MI). We demonstrated that administration of both cEVs and nEVs significantly improved cardiac function, reduced the scar size, and alleviated inflammatory infiltration into the heart. In addition, cardiomyocyte apoptosis was inhibited, whereas angiogenesis was enhanced in the hearts receiving cEVs or nEVs treatment. Moreover, intramyocardial injection of cEVs displayed much better cardiac protective efficacy than nEVs in murine MI models. RNA-seq and protein-protein interaction (PPI) network analysis revealed the protective mRNA clusters in both cEVs and nEVs. These mRNAs were involved in multiple signaling pathways, which may synergistically orchestrate to prevent the heart from further damage post MI. CONCLUSIONS: Collectively, our results indicated that EVs derived from normal heart tissue may represent a promising strategy for cardiac protection in ischemic heart diseases. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13062-023-00429-y.
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spelling pubmed-106553532023-11-17 Extracellular vesicles derived from different tissues attenuate cardiac dysfunction in murine MI models Liu, Xuan Shi, Shanshan Geng, Xuedi Wang, Enhao Meng, Qingshu Li, Mimi Lin, Fang Ma, Xiaoxue Han, Wei Zhou, Xiaohui Biol Direct Research BACKGROUND: Extracellular vesicles (EVs) derived from various cell sources exert cardioprotective effects during cardiac ischemic injury. Our previous study confirmed that EVs derived from ischemic-reperfusion injured heart tissue aggravated cardiac inflammation and dysfunction. However, the role of EVs derived from normal cardiac tissue in myocardial ischemic injury remains elusive. RESULTS: In the present study, normal heart-derived EVs (cEVs) and kidney-derived EVs (nEVs) were isolated and intramyocardially injected into mice after myocardial infarction (MI). We demonstrated that administration of both cEVs and nEVs significantly improved cardiac function, reduced the scar size, and alleviated inflammatory infiltration into the heart. In addition, cardiomyocyte apoptosis was inhibited, whereas angiogenesis was enhanced in the hearts receiving cEVs or nEVs treatment. Moreover, intramyocardial injection of cEVs displayed much better cardiac protective efficacy than nEVs in murine MI models. RNA-seq and protein-protein interaction (PPI) network analysis revealed the protective mRNA clusters in both cEVs and nEVs. These mRNAs were involved in multiple signaling pathways, which may synergistically orchestrate to prevent the heart from further damage post MI. CONCLUSIONS: Collectively, our results indicated that EVs derived from normal heart tissue may represent a promising strategy for cardiac protection in ischemic heart diseases. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13062-023-00429-y. BioMed Central 2023-11-17 /pmc/articles/PMC10655353/ /pubmed/37978390 http://dx.doi.org/10.1186/s13062-023-00429-y Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Liu, Xuan
Shi, Shanshan
Geng, Xuedi
Wang, Enhao
Meng, Qingshu
Li, Mimi
Lin, Fang
Ma, Xiaoxue
Han, Wei
Zhou, Xiaohui
Extracellular vesicles derived from different tissues attenuate cardiac dysfunction in murine MI models
title Extracellular vesicles derived from different tissues attenuate cardiac dysfunction in murine MI models
title_full Extracellular vesicles derived from different tissues attenuate cardiac dysfunction in murine MI models
title_fullStr Extracellular vesicles derived from different tissues attenuate cardiac dysfunction in murine MI models
title_full_unstemmed Extracellular vesicles derived from different tissues attenuate cardiac dysfunction in murine MI models
title_short Extracellular vesicles derived from different tissues attenuate cardiac dysfunction in murine MI models
title_sort extracellular vesicles derived from different tissues attenuate cardiac dysfunction in murine mi models
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10655353/
https://www.ncbi.nlm.nih.gov/pubmed/37978390
http://dx.doi.org/10.1186/s13062-023-00429-y
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