Cargando…
Food-grade titanium dioxide and zinc oxide nanoparticles induce toxicity and cardiac damage after oral exposure in rats
BACKGROUND: Metallic nanoparticles (NPs) are widely used as food additives for human consumption. NPs reach the bloodstream given their small size, getting in contact with all body organs and cells. NPs have adverse effects on the respiratory and intestinal tract; however, few studies have focused o...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10655394/ https://www.ncbi.nlm.nih.gov/pubmed/37978398 http://dx.doi.org/10.1186/s12989-023-00553-7 |
_version_ | 1785147937817362432 |
---|---|
author | Herrera-Rodríguez, Manuel Alejandro del Pilar Ramos-Godinez, María Cano-Martínez, Agustina Segura, Francisco Correa Ruiz-Ramírez, Angélica Pavón, Natalia Lira-Silva, Elizabeth Bautista-Pérez, Rocío Thomas, Rosina Sánchez Delgado-Buenrostro, Norma Laura Chirino, Yolanda Irasema López-Marure, Rebeca |
author_facet | Herrera-Rodríguez, Manuel Alejandro del Pilar Ramos-Godinez, María Cano-Martínez, Agustina Segura, Francisco Correa Ruiz-Ramírez, Angélica Pavón, Natalia Lira-Silva, Elizabeth Bautista-Pérez, Rocío Thomas, Rosina Sánchez Delgado-Buenrostro, Norma Laura Chirino, Yolanda Irasema López-Marure, Rebeca |
author_sort | Herrera-Rodríguez, Manuel Alejandro |
collection | PubMed |
description | BACKGROUND: Metallic nanoparticles (NPs) are widely used as food additives for human consumption. NPs reach the bloodstream given their small size, getting in contact with all body organs and cells. NPs have adverse effects on the respiratory and intestinal tract; however, few studies have focused on the toxic consequences of orally ingested metallic NPs on the cardiovascular system. Here, the effects of two food-grade additives on the cardiovascular system were analyzed. METHODS: Titanium dioxide labeled as E171 and zinc oxide (ZnO) NPs were orally administered to Wistar rats using an esophageal cannula at 10 mg/kg bw every other day for 90 days. We evaluated cardiac cell morphology and death, expression of apoptotic and autophagic proteins in cardiac mitochondria, mitochondrial dysfunction, and concentration of metals on cardiac tissue. RESULTS: Heart histology showed important morphological changes such as presence of cellular infiltrates, collagen deposition and mitochondrial alterations in hearts from rats exposed to E171 and ZnO NPs. Intracellular Cyt-C levels dropped, while TUNEL positive cells increased. No significant changes in the expression of inflammatory cytokines were detected. Both NPs altered mitochondrial function indicating cardiac dysfunction, which was associated with an elevated concentration of calcium. ZnO NPs induced expression of caspases 3 and 9 and two autophagic proteins, LC3B and beclin-1, and had the strongest effect compared to E171. CONCLUSIONS: E171 and ZnO NPs induce adverse cardiovascular effects in rats after 90 days of exposure, thus food intake containing these additives, should be taken into consideration, since they translocate into the bloodstream and cause cardiovascular damage. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12989-023-00553-7. |
format | Online Article Text |
id | pubmed-10655394 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-106553942023-11-17 Food-grade titanium dioxide and zinc oxide nanoparticles induce toxicity and cardiac damage after oral exposure in rats Herrera-Rodríguez, Manuel Alejandro del Pilar Ramos-Godinez, María Cano-Martínez, Agustina Segura, Francisco Correa Ruiz-Ramírez, Angélica Pavón, Natalia Lira-Silva, Elizabeth Bautista-Pérez, Rocío Thomas, Rosina Sánchez Delgado-Buenrostro, Norma Laura Chirino, Yolanda Irasema López-Marure, Rebeca Part Fibre Toxicol Research BACKGROUND: Metallic nanoparticles (NPs) are widely used as food additives for human consumption. NPs reach the bloodstream given their small size, getting in contact with all body organs and cells. NPs have adverse effects on the respiratory and intestinal tract; however, few studies have focused on the toxic consequences of orally ingested metallic NPs on the cardiovascular system. Here, the effects of two food-grade additives on the cardiovascular system were analyzed. METHODS: Titanium dioxide labeled as E171 and zinc oxide (ZnO) NPs were orally administered to Wistar rats using an esophageal cannula at 10 mg/kg bw every other day for 90 days. We evaluated cardiac cell morphology and death, expression of apoptotic and autophagic proteins in cardiac mitochondria, mitochondrial dysfunction, and concentration of metals on cardiac tissue. RESULTS: Heart histology showed important morphological changes such as presence of cellular infiltrates, collagen deposition and mitochondrial alterations in hearts from rats exposed to E171 and ZnO NPs. Intracellular Cyt-C levels dropped, while TUNEL positive cells increased. No significant changes in the expression of inflammatory cytokines were detected. Both NPs altered mitochondrial function indicating cardiac dysfunction, which was associated with an elevated concentration of calcium. ZnO NPs induced expression of caspases 3 and 9 and two autophagic proteins, LC3B and beclin-1, and had the strongest effect compared to E171. CONCLUSIONS: E171 and ZnO NPs induce adverse cardiovascular effects in rats after 90 days of exposure, thus food intake containing these additives, should be taken into consideration, since they translocate into the bloodstream and cause cardiovascular damage. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12989-023-00553-7. BioMed Central 2023-11-17 /pmc/articles/PMC10655394/ /pubmed/37978398 http://dx.doi.org/10.1186/s12989-023-00553-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Herrera-Rodríguez, Manuel Alejandro del Pilar Ramos-Godinez, María Cano-Martínez, Agustina Segura, Francisco Correa Ruiz-Ramírez, Angélica Pavón, Natalia Lira-Silva, Elizabeth Bautista-Pérez, Rocío Thomas, Rosina Sánchez Delgado-Buenrostro, Norma Laura Chirino, Yolanda Irasema López-Marure, Rebeca Food-grade titanium dioxide and zinc oxide nanoparticles induce toxicity and cardiac damage after oral exposure in rats |
title | Food-grade titanium dioxide and zinc oxide nanoparticles induce toxicity and cardiac damage after oral exposure in rats |
title_full | Food-grade titanium dioxide and zinc oxide nanoparticles induce toxicity and cardiac damage after oral exposure in rats |
title_fullStr | Food-grade titanium dioxide and zinc oxide nanoparticles induce toxicity and cardiac damage after oral exposure in rats |
title_full_unstemmed | Food-grade titanium dioxide and zinc oxide nanoparticles induce toxicity and cardiac damage after oral exposure in rats |
title_short | Food-grade titanium dioxide and zinc oxide nanoparticles induce toxicity and cardiac damage after oral exposure in rats |
title_sort | food-grade titanium dioxide and zinc oxide nanoparticles induce toxicity and cardiac damage after oral exposure in rats |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10655394/ https://www.ncbi.nlm.nih.gov/pubmed/37978398 http://dx.doi.org/10.1186/s12989-023-00553-7 |
work_keys_str_mv | AT herrerarodriguezmanuelalejandro foodgradetitaniumdioxideandzincoxidenanoparticlesinducetoxicityandcardiacdamageafteroralexposureinrats AT delpilarramosgodinezmaria foodgradetitaniumdioxideandzincoxidenanoparticlesinducetoxicityandcardiacdamageafteroralexposureinrats AT canomartinezagustina foodgradetitaniumdioxideandzincoxidenanoparticlesinducetoxicityandcardiacdamageafteroralexposureinrats AT segurafranciscocorrea foodgradetitaniumdioxideandzincoxidenanoparticlesinducetoxicityandcardiacdamageafteroralexposureinrats AT ruizramirezangelica foodgradetitaniumdioxideandzincoxidenanoparticlesinducetoxicityandcardiacdamageafteroralexposureinrats AT pavonnatalia foodgradetitaniumdioxideandzincoxidenanoparticlesinducetoxicityandcardiacdamageafteroralexposureinrats AT lirasilvaelizabeth foodgradetitaniumdioxideandzincoxidenanoparticlesinducetoxicityandcardiacdamageafteroralexposureinrats AT bautistaperezrocio foodgradetitaniumdioxideandzincoxidenanoparticlesinducetoxicityandcardiacdamageafteroralexposureinrats AT thomasrosinasanchez foodgradetitaniumdioxideandzincoxidenanoparticlesinducetoxicityandcardiacdamageafteroralexposureinrats AT delgadobuenrostronormalaura foodgradetitaniumdioxideandzincoxidenanoparticlesinducetoxicityandcardiacdamageafteroralexposureinrats AT chirinoyolandairasema foodgradetitaniumdioxideandzincoxidenanoparticlesinducetoxicityandcardiacdamageafteroralexposureinrats AT lopezmarurerebeca foodgradetitaniumdioxideandzincoxidenanoparticlesinducetoxicityandcardiacdamageafteroralexposureinrats |