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Beyond the surface: exploring the mycobiome of Norway spruce under drought stress and with Heterobasidion parviporum
The mycobiome, comprising fungi inhabiting plants, potentially plays a crucial role in tree health and survival amidst environmental stressors like climate change and pathogenic fungi. Understanding the intricate relationships between trees and their microbial communities is essential for developing...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10655427/ https://www.ncbi.nlm.nih.gov/pubmed/37978432 http://dx.doi.org/10.1186/s12866-023-03099-y |
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author | Durodola, Blessing Blumenstein, Kathrin Akinbobola, Adedolapo Kolehmainen, Anna Chano, Victor Gailing, Oliver Terhonen, Eeva |
author_facet | Durodola, Blessing Blumenstein, Kathrin Akinbobola, Adedolapo Kolehmainen, Anna Chano, Victor Gailing, Oliver Terhonen, Eeva |
author_sort | Durodola, Blessing |
collection | PubMed |
description | The mycobiome, comprising fungi inhabiting plants, potentially plays a crucial role in tree health and survival amidst environmental stressors like climate change and pathogenic fungi. Understanding the intricate relationships between trees and their microbial communities is essential for developing effective strategies to bolster the resilience and well-being of forest ecosystems as we adopt more sustainable forest management practices. The mycobiome can be considered an integral aspect of a tree’s biology, closely linked to its genotype. To explore the influence of host genetics and environmental factors on fungal composition, we examined the mycobiome associated with phloem and roots of Norway spruce (Picea abies (L.) Karst.) cuttings under varying watering conditions. To test the “mycobiome-associated-fitness” hypothesis, we compared seedlings artificially inoculated with Heterobasidion parviporum and control plants to evaluate mycobiome interaction on necrosis development. We aimed to 1) identify specific mycobiome species for the Norway spruce genotypes/families within the phloem and root tissues and their interactions with H. parviporum and 2) assess stability in the mycobiome species composition under abiotic disturbances (reduced water availability). The mycobiome was analyzed by sequencing the ribosomal ITS2 region. Our results revealed significant variations in the diversity and prevalence of the phloem mycobiome among different Norway spruce genotypes, highlighting the considerable impact of genetic variation on the composition and diversity of the phloem mycobiome. Additionally, specific mycobiome genera in the phloem showed variations in response to water availability, indicating the influence of environmental conditions on the relative proportion of certain fungal genera in Norway spruce trees. In the root mycobiome, key fungi such as Phialocephala fortinii and Paraphaeosphaeria neglecta were identified as conferring inhibitory effects against H. parviporum growth in Norway spruce genotypes. Furthermore, certain endophytes demonstrated greater stability in root ecosystems under low water conditions than ectomycorrhizal fungi. This knowledge can contribute to developing sustainable forest management practices that enhance the well-being of trees and their ecosystems, ultimately bolstering forest resilience. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-023-03099-y. |
format | Online Article Text |
id | pubmed-10655427 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-106554272023-11-17 Beyond the surface: exploring the mycobiome of Norway spruce under drought stress and with Heterobasidion parviporum Durodola, Blessing Blumenstein, Kathrin Akinbobola, Adedolapo Kolehmainen, Anna Chano, Victor Gailing, Oliver Terhonen, Eeva BMC Microbiol Research The mycobiome, comprising fungi inhabiting plants, potentially plays a crucial role in tree health and survival amidst environmental stressors like climate change and pathogenic fungi. Understanding the intricate relationships between trees and their microbial communities is essential for developing effective strategies to bolster the resilience and well-being of forest ecosystems as we adopt more sustainable forest management practices. The mycobiome can be considered an integral aspect of a tree’s biology, closely linked to its genotype. To explore the influence of host genetics and environmental factors on fungal composition, we examined the mycobiome associated with phloem and roots of Norway spruce (Picea abies (L.) Karst.) cuttings under varying watering conditions. To test the “mycobiome-associated-fitness” hypothesis, we compared seedlings artificially inoculated with Heterobasidion parviporum and control plants to evaluate mycobiome interaction on necrosis development. We aimed to 1) identify specific mycobiome species for the Norway spruce genotypes/families within the phloem and root tissues and their interactions with H. parviporum and 2) assess stability in the mycobiome species composition under abiotic disturbances (reduced water availability). The mycobiome was analyzed by sequencing the ribosomal ITS2 region. Our results revealed significant variations in the diversity and prevalence of the phloem mycobiome among different Norway spruce genotypes, highlighting the considerable impact of genetic variation on the composition and diversity of the phloem mycobiome. Additionally, specific mycobiome genera in the phloem showed variations in response to water availability, indicating the influence of environmental conditions on the relative proportion of certain fungal genera in Norway spruce trees. In the root mycobiome, key fungi such as Phialocephala fortinii and Paraphaeosphaeria neglecta were identified as conferring inhibitory effects against H. parviporum growth in Norway spruce genotypes. Furthermore, certain endophytes demonstrated greater stability in root ecosystems under low water conditions than ectomycorrhizal fungi. This knowledge can contribute to developing sustainable forest management practices that enhance the well-being of trees and their ecosystems, ultimately bolstering forest resilience. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-023-03099-y. BioMed Central 2023-11-17 /pmc/articles/PMC10655427/ /pubmed/37978432 http://dx.doi.org/10.1186/s12866-023-03099-y Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Durodola, Blessing Blumenstein, Kathrin Akinbobola, Adedolapo Kolehmainen, Anna Chano, Victor Gailing, Oliver Terhonen, Eeva Beyond the surface: exploring the mycobiome of Norway spruce under drought stress and with Heterobasidion parviporum |
title | Beyond the surface: exploring the mycobiome of Norway spruce under drought stress and with Heterobasidion parviporum |
title_full | Beyond the surface: exploring the mycobiome of Norway spruce under drought stress and with Heterobasidion parviporum |
title_fullStr | Beyond the surface: exploring the mycobiome of Norway spruce under drought stress and with Heterobasidion parviporum |
title_full_unstemmed | Beyond the surface: exploring the mycobiome of Norway spruce under drought stress and with Heterobasidion parviporum |
title_short | Beyond the surface: exploring the mycobiome of Norway spruce under drought stress and with Heterobasidion parviporum |
title_sort | beyond the surface: exploring the mycobiome of norway spruce under drought stress and with heterobasidion parviporum |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10655427/ https://www.ncbi.nlm.nih.gov/pubmed/37978432 http://dx.doi.org/10.1186/s12866-023-03099-y |
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