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Atrazine Degradation Using Immobilized Triazine Hydrolase from Arthrobacter aurescens TC1 in Mesoporous Silica Nanomaterials
[Image: see text] Triazine hydrolase fromArthrobacter aurescens TC1 (TrzN) was successfully immobilized on mesoporous silica nanomaterials (MSNs) for the first time. For both nonfunctionalized MSNs and MSNs functionalized with Zn(II), three pore sizes were evaluated for their ability to immobilize w...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10655591/ https://www.ncbi.nlm.nih.gov/pubmed/38028742 http://dx.doi.org/10.1021/acsenvironau.3c00036 |
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author | Diviesti, Karla Russell-Parks, Glory A. Trewyn, Brian G. Holz, Richard C. |
author_facet | Diviesti, Karla Russell-Parks, Glory A. Trewyn, Brian G. Holz, Richard C. |
author_sort | Diviesti, Karla |
collection | PubMed |
description | [Image: see text] Triazine hydrolase fromArthrobacter aurescens TC1 (TrzN) was successfully immobilized on mesoporous silica nanomaterials (MSNs) for the first time. For both nonfunctionalized MSNs and MSNs functionalized with Zn(II), three pore sizes were evaluated for their ability to immobilize wild-type TrzN: Mobile composition of matter no. 41 (small, 3 nm pores), mesoporous silica nanoparticle material with 10 nm pore diameter (MSN-10) (medium, 6–12 nm pores), and pore-expanded MSN-10 (large, 15–30 nm pores). Of these six TrzN:MSN biomaterials, it was shown that TrzN:MSN-10 was the most active (3.8 ± 0.4 × 10(–5) U/mg) toward the hydrolysis of a 50 μM atrazine solution at 25 °C. The TrzN:MSN-10 biomaterial was then coated in chitosan (TrzN:MSN-10:Chit) as chitosan has been shown to increase stability in extreme conditions such as low/high pH, heat shock, and the presence of organic solvents. TrzN:MSN-10:Chit was shown to be a superior TrzN biomaterial to TrzN:MSN-10 as it exhibited higher activity under all storage conditions, in the presence of 20% MeOH, at low and high pH values, and at elevated temperatures up to 80 °C. Finally, the TrzN:MSN-10:Chit biomaterial was shown to be fully active in river water, which establishes it as a functional biomaterial under actual field conditions. A combination of these data indicate that the TrzN:MSN-10:Chit biomaterial exhibited the best overall catalytic profile making it a promising biocatalyst for the bioremediation of atrazine. |
format | Online Article Text |
id | pubmed-10655591 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-106555912023-11-01 Atrazine Degradation Using Immobilized Triazine Hydrolase from Arthrobacter aurescens TC1 in Mesoporous Silica Nanomaterials Diviesti, Karla Russell-Parks, Glory A. Trewyn, Brian G. Holz, Richard C. ACS Environ Au [Image: see text] Triazine hydrolase fromArthrobacter aurescens TC1 (TrzN) was successfully immobilized on mesoporous silica nanomaterials (MSNs) for the first time. For both nonfunctionalized MSNs and MSNs functionalized with Zn(II), three pore sizes were evaluated for their ability to immobilize wild-type TrzN: Mobile composition of matter no. 41 (small, 3 nm pores), mesoporous silica nanoparticle material with 10 nm pore diameter (MSN-10) (medium, 6–12 nm pores), and pore-expanded MSN-10 (large, 15–30 nm pores). Of these six TrzN:MSN biomaterials, it was shown that TrzN:MSN-10 was the most active (3.8 ± 0.4 × 10(–5) U/mg) toward the hydrolysis of a 50 μM atrazine solution at 25 °C. The TrzN:MSN-10 biomaterial was then coated in chitosan (TrzN:MSN-10:Chit) as chitosan has been shown to increase stability in extreme conditions such as low/high pH, heat shock, and the presence of organic solvents. TrzN:MSN-10:Chit was shown to be a superior TrzN biomaterial to TrzN:MSN-10 as it exhibited higher activity under all storage conditions, in the presence of 20% MeOH, at low and high pH values, and at elevated temperatures up to 80 °C. Finally, the TrzN:MSN-10:Chit biomaterial was shown to be fully active in river water, which establishes it as a functional biomaterial under actual field conditions. A combination of these data indicate that the TrzN:MSN-10:Chit biomaterial exhibited the best overall catalytic profile making it a promising biocatalyst for the bioremediation of atrazine. American Chemical Society 2023-11-01 /pmc/articles/PMC10655591/ /pubmed/38028742 http://dx.doi.org/10.1021/acsenvironau.3c00036 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Diviesti, Karla Russell-Parks, Glory A. Trewyn, Brian G. Holz, Richard C. Atrazine Degradation Using Immobilized Triazine Hydrolase from Arthrobacter aurescens TC1 in Mesoporous Silica Nanomaterials |
title | Atrazine Degradation
Using Immobilized Triazine Hydrolase
from Arthrobacter aurescens TC1 in
Mesoporous Silica Nanomaterials |
title_full | Atrazine Degradation
Using Immobilized Triazine Hydrolase
from Arthrobacter aurescens TC1 in
Mesoporous Silica Nanomaterials |
title_fullStr | Atrazine Degradation
Using Immobilized Triazine Hydrolase
from Arthrobacter aurescens TC1 in
Mesoporous Silica Nanomaterials |
title_full_unstemmed | Atrazine Degradation
Using Immobilized Triazine Hydrolase
from Arthrobacter aurescens TC1 in
Mesoporous Silica Nanomaterials |
title_short | Atrazine Degradation
Using Immobilized Triazine Hydrolase
from Arthrobacter aurescens TC1 in
Mesoporous Silica Nanomaterials |
title_sort | atrazine degradation
using immobilized triazine hydrolase
from arthrobacter aurescens tc1 in
mesoporous silica nanomaterials |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10655591/ https://www.ncbi.nlm.nih.gov/pubmed/38028742 http://dx.doi.org/10.1021/acsenvironau.3c00036 |
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