Cargando…

Atrazine Degradation Using Immobilized Triazine Hydrolase from Arthrobacter aurescens TC1 in Mesoporous Silica Nanomaterials

[Image: see text] Triazine hydrolase fromArthrobacter aurescens TC1 (TrzN) was successfully immobilized on mesoporous silica nanomaterials (MSNs) for the first time. For both nonfunctionalized MSNs and MSNs functionalized with Zn(II), three pore sizes were evaluated for their ability to immobilize w...

Descripción completa

Detalles Bibliográficos
Autores principales: Diviesti, Karla, Russell-Parks, Glory A., Trewyn, Brian G., Holz, Richard C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10655591/
https://www.ncbi.nlm.nih.gov/pubmed/38028742
http://dx.doi.org/10.1021/acsenvironau.3c00036
_version_ 1785136852630503424
author Diviesti, Karla
Russell-Parks, Glory A.
Trewyn, Brian G.
Holz, Richard C.
author_facet Diviesti, Karla
Russell-Parks, Glory A.
Trewyn, Brian G.
Holz, Richard C.
author_sort Diviesti, Karla
collection PubMed
description [Image: see text] Triazine hydrolase fromArthrobacter aurescens TC1 (TrzN) was successfully immobilized on mesoporous silica nanomaterials (MSNs) for the first time. For both nonfunctionalized MSNs and MSNs functionalized with Zn(II), three pore sizes were evaluated for their ability to immobilize wild-type TrzN: Mobile composition of matter no. 41 (small, 3 nm pores), mesoporous silica nanoparticle material with 10 nm pore diameter (MSN-10) (medium, 6–12 nm pores), and pore-expanded MSN-10 (large, 15–30 nm pores). Of these six TrzN:MSN biomaterials, it was shown that TrzN:MSN-10 was the most active (3.8 ± 0.4 × 10(–5) U/mg) toward the hydrolysis of a 50 μM atrazine solution at 25 °C. The TrzN:MSN-10 biomaterial was then coated in chitosan (TrzN:MSN-10:Chit) as chitosan has been shown to increase stability in extreme conditions such as low/high pH, heat shock, and the presence of organic solvents. TrzN:MSN-10:Chit was shown to be a superior TrzN biomaterial to TrzN:MSN-10 as it exhibited higher activity under all storage conditions, in the presence of 20% MeOH, at low and high pH values, and at elevated temperatures up to 80 °C. Finally, the TrzN:MSN-10:Chit biomaterial was shown to be fully active in river water, which establishes it as a functional biomaterial under actual field conditions. A combination of these data indicate that the TrzN:MSN-10:Chit biomaterial exhibited the best overall catalytic profile making it a promising biocatalyst for the bioremediation of atrazine.
format Online
Article
Text
id pubmed-10655591
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-106555912023-11-01 Atrazine Degradation Using Immobilized Triazine Hydrolase from Arthrobacter aurescens TC1 in Mesoporous Silica Nanomaterials Diviesti, Karla Russell-Parks, Glory A. Trewyn, Brian G. Holz, Richard C. ACS Environ Au [Image: see text] Triazine hydrolase fromArthrobacter aurescens TC1 (TrzN) was successfully immobilized on mesoporous silica nanomaterials (MSNs) for the first time. For both nonfunctionalized MSNs and MSNs functionalized with Zn(II), three pore sizes were evaluated for their ability to immobilize wild-type TrzN: Mobile composition of matter no. 41 (small, 3 nm pores), mesoporous silica nanoparticle material with 10 nm pore diameter (MSN-10) (medium, 6–12 nm pores), and pore-expanded MSN-10 (large, 15–30 nm pores). Of these six TrzN:MSN biomaterials, it was shown that TrzN:MSN-10 was the most active (3.8 ± 0.4 × 10(–5) U/mg) toward the hydrolysis of a 50 μM atrazine solution at 25 °C. The TrzN:MSN-10 biomaterial was then coated in chitosan (TrzN:MSN-10:Chit) as chitosan has been shown to increase stability in extreme conditions such as low/high pH, heat shock, and the presence of organic solvents. TrzN:MSN-10:Chit was shown to be a superior TrzN biomaterial to TrzN:MSN-10 as it exhibited higher activity under all storage conditions, in the presence of 20% MeOH, at low and high pH values, and at elevated temperatures up to 80 °C. Finally, the TrzN:MSN-10:Chit biomaterial was shown to be fully active in river water, which establishes it as a functional biomaterial under actual field conditions. A combination of these data indicate that the TrzN:MSN-10:Chit biomaterial exhibited the best overall catalytic profile making it a promising biocatalyst for the bioremediation of atrazine. American Chemical Society 2023-11-01 /pmc/articles/PMC10655591/ /pubmed/38028742 http://dx.doi.org/10.1021/acsenvironau.3c00036 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Diviesti, Karla
Russell-Parks, Glory A.
Trewyn, Brian G.
Holz, Richard C.
Atrazine Degradation Using Immobilized Triazine Hydrolase from Arthrobacter aurescens TC1 in Mesoporous Silica Nanomaterials
title Atrazine Degradation Using Immobilized Triazine Hydrolase from Arthrobacter aurescens TC1 in Mesoporous Silica Nanomaterials
title_full Atrazine Degradation Using Immobilized Triazine Hydrolase from Arthrobacter aurescens TC1 in Mesoporous Silica Nanomaterials
title_fullStr Atrazine Degradation Using Immobilized Triazine Hydrolase from Arthrobacter aurescens TC1 in Mesoporous Silica Nanomaterials
title_full_unstemmed Atrazine Degradation Using Immobilized Triazine Hydrolase from Arthrobacter aurescens TC1 in Mesoporous Silica Nanomaterials
title_short Atrazine Degradation Using Immobilized Triazine Hydrolase from Arthrobacter aurescens TC1 in Mesoporous Silica Nanomaterials
title_sort atrazine degradation using immobilized triazine hydrolase from arthrobacter aurescens tc1 in mesoporous silica nanomaterials
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10655591/
https://www.ncbi.nlm.nih.gov/pubmed/38028742
http://dx.doi.org/10.1021/acsenvironau.3c00036
work_keys_str_mv AT diviestikarla atrazinedegradationusingimmobilizedtriazinehydrolasefromarthrobacteraurescenstc1inmesoporoussilicananomaterials
AT russellparksglorya atrazinedegradationusingimmobilizedtriazinehydrolasefromarthrobacteraurescenstc1inmesoporoussilicananomaterials
AT trewynbriang atrazinedegradationusingimmobilizedtriazinehydrolasefromarthrobacteraurescenstc1inmesoporoussilicananomaterials
AT holzrichardc atrazinedegradationusingimmobilizedtriazinehydrolasefromarthrobacteraurescenstc1inmesoporoussilicananomaterials