Molecular Mechanisms of the miR396b-GRF1 Module Underlying Rooting Regulation in Acer rubrum L.

Rooting and root development in Acer rubrum have important effects on overall growth. A. rubrum does not take root easily in natural conditions. In this study, the mechanisms of the miR396b-GRF1 module underlying rooting regulation in A. rubrum were studied. The subcellular localization and transcri...

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Detalles Bibliográficos
Autores principales: Zhang, Manyu, Li, Huiju, Zhu, Huiyu, Zhao, Hewen, Zhang, Kezhong, Ge, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2023
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10655668/
https://www.ncbi.nlm.nih.gov/pubmed/38020534
http://dx.doi.org/10.1177/11769343231211071
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author Zhang, Manyu
Li, Huiju
Zhu, Huiyu
Zhao, Hewen
Zhang, Kezhong
Ge, Wei
author_facet Zhang, Manyu
Li, Huiju
Zhu, Huiyu
Zhao, Hewen
Zhang, Kezhong
Ge, Wei
author_sort Zhang, Manyu
collection PubMed
description Rooting and root development in Acer rubrum have important effects on overall growth. A. rubrum does not take root easily in natural conditions. In this study, the mechanisms of the miR396b-GRF1 module underlying rooting regulation in A. rubrum were studied. The subcellular localization and transcriptional activation of miR396b and its target gene growth regulating factor 1 (GRF1) were investigated. These experiments showed that GRF1 was localized in the nucleus and had transcriptional activation activity. Functional validation experiments in transgenic plants demonstrated that overexpression of Ar-miR396b inhibited adventitious root growth, whereas overexpression of ArGRF1 increased adventitious root growth. These results help clarify the molecular regulatory mechanisms underlying adventitious root growth in A. rubrum and provide some new insights into the rooting rate in this species.
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spelling pubmed-106556682023-11-15 Molecular Mechanisms of the miR396b-GRF1 Module Underlying Rooting Regulation in Acer rubrum L. Zhang, Manyu Li, Huiju Zhu, Huiyu Zhao, Hewen Zhang, Kezhong Ge, Wei Evol Bioinform Online Original Research Rooting and root development in Acer rubrum have important effects on overall growth. A. rubrum does not take root easily in natural conditions. In this study, the mechanisms of the miR396b-GRF1 module underlying rooting regulation in A. rubrum were studied. The subcellular localization and transcriptional activation of miR396b and its target gene growth regulating factor 1 (GRF1) were investigated. These experiments showed that GRF1 was localized in the nucleus and had transcriptional activation activity. Functional validation experiments in transgenic plants demonstrated that overexpression of Ar-miR396b inhibited adventitious root growth, whereas overexpression of ArGRF1 increased adventitious root growth. These results help clarify the molecular regulatory mechanisms underlying adventitious root growth in A. rubrum and provide some new insights into the rooting rate in this species. SAGE Publications 2023-11-15 /pmc/articles/PMC10655668/ /pubmed/38020534 http://dx.doi.org/10.1177/11769343231211071 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Research
Zhang, Manyu
Li, Huiju
Zhu, Huiyu
Zhao, Hewen
Zhang, Kezhong
Ge, Wei
Molecular Mechanisms of the miR396b-GRF1 Module Underlying Rooting Regulation in Acer rubrum L.
title Molecular Mechanisms of the miR396b-GRF1 Module Underlying Rooting Regulation in Acer rubrum L.
title_full Molecular Mechanisms of the miR396b-GRF1 Module Underlying Rooting Regulation in Acer rubrum L.
title_fullStr Molecular Mechanisms of the miR396b-GRF1 Module Underlying Rooting Regulation in Acer rubrum L.
title_full_unstemmed Molecular Mechanisms of the miR396b-GRF1 Module Underlying Rooting Regulation in Acer rubrum L.
title_short Molecular Mechanisms of the miR396b-GRF1 Module Underlying Rooting Regulation in Acer rubrum L.
title_sort molecular mechanisms of the mir396b-grf1 module underlying rooting regulation in acer rubrum l.
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10655668/
https://www.ncbi.nlm.nih.gov/pubmed/38020534
http://dx.doi.org/10.1177/11769343231211071
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