Cargando…

Molecular classification of muscle-invasive bladder cancer based on a simplified immunohistochemical panel using GATA3, CK5/6, and p16

The choice of therapy for muscle-invasive bladder cancer (MIBC) could be influenced by the tumor’s molecular subtype. Currently, well-defined consensus subtypes are based on tumor microarray mRNA data. Clearly defined and easy-to-use surrogate molecular subtypes, based on immunohistochemistry (IHC)...

Descripción completa

Detalles Bibliográficos
Autores principales: Terlević, Robert, Ulamec, Monika, Štimac, Goran, Murgić, Jure, Krušlin, Božo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10655881/
https://www.ncbi.nlm.nih.gov/pubmed/37389960
http://dx.doi.org/10.17305/bb.2023.9242
_version_ 1785147994928054272
author Terlević, Robert
Ulamec, Monika
Štimac, Goran
Murgić, Jure
Krušlin, Božo
author_facet Terlević, Robert
Ulamec, Monika
Štimac, Goran
Murgić, Jure
Krušlin, Božo
author_sort Terlević, Robert
collection PubMed
description The choice of therapy for muscle-invasive bladder cancer (MIBC) could be influenced by the tumor’s molecular subtype. Currently, well-defined consensus subtypes are based on tumor microarray mRNA data. Clearly defined and easy-to-use surrogate molecular subtypes, based on immunohistochemistry (IHC) performed on whole slides, are needed to make subtyping cost-effective and useful in routine work and future research. To aid in the development of a simple immunohistochemical classifier, a retrospective single-center series of 92 cases of localized bladder cancer was identified. Routine IHC for GATA 3 transcription factor (GATA3), cytokeratins 5 and 6 (CK5/6), and p16 was performed on whole tissue blocks containing muscle-invasive disease. Electronic medical records were retrieved and searched for clinical variables, treatment, and survival data. The mean age was 69.6 years, and 73% were males. Conservative treatment was used in 55% of cases, while cystectomy with chemotherapy was used in 45%. GATA3 and CK5/6 expression divided cases into broad luminal and basal subtypes, respectively, while p16 expression was used to subclassify luminal cases into luminal papillary and luminal unstable types according to the consensus molecular classification. When subtyped in this way, GATA3 and CK5/6 negative cases showed worse overall survival. Molecular subtyping of MIBC on whole slides containing muscle-invasive tumor using only three commonly used consensus-based antibodies is a feasible and cost-effective method for detecting subtypes of invasive bladder cancer. Future work combining morphological analysis and IHC is needed to fully translate the consensus molecular classification into a comprehensive, cost-effective subtyping strategy.
format Online
Article
Text
id pubmed-10655881
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
record_format MEDLINE/PubMed
spelling pubmed-106558812023-12-01 Molecular classification of muscle-invasive bladder cancer based on a simplified immunohistochemical panel using GATA3, CK5/6, and p16 Terlević, Robert Ulamec, Monika Štimac, Goran Murgić, Jure Krušlin, Božo Biomol Biomed Research Article The choice of therapy for muscle-invasive bladder cancer (MIBC) could be influenced by the tumor’s molecular subtype. Currently, well-defined consensus subtypes are based on tumor microarray mRNA data. Clearly defined and easy-to-use surrogate molecular subtypes, based on immunohistochemistry (IHC) performed on whole slides, are needed to make subtyping cost-effective and useful in routine work and future research. To aid in the development of a simple immunohistochemical classifier, a retrospective single-center series of 92 cases of localized bladder cancer was identified. Routine IHC for GATA 3 transcription factor (GATA3), cytokeratins 5 and 6 (CK5/6), and p16 was performed on whole tissue blocks containing muscle-invasive disease. Electronic medical records were retrieved and searched for clinical variables, treatment, and survival data. The mean age was 69.6 years, and 73% were males. Conservative treatment was used in 55% of cases, while cystectomy with chemotherapy was used in 45%. GATA3 and CK5/6 expression divided cases into broad luminal and basal subtypes, respectively, while p16 expression was used to subclassify luminal cases into luminal papillary and luminal unstable types according to the consensus molecular classification. When subtyped in this way, GATA3 and CK5/6 negative cases showed worse overall survival. Molecular subtyping of MIBC on whole slides containing muscle-invasive tumor using only three commonly used consensus-based antibodies is a feasible and cost-effective method for detecting subtypes of invasive bladder cancer. Future work combining morphological analysis and IHC is needed to fully translate the consensus molecular classification into a comprehensive, cost-effective subtyping strategy. Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina 2023-12-01 2023-12-01 /pmc/articles/PMC10655881/ /pubmed/37389960 http://dx.doi.org/10.17305/bb.2023.9242 Text en © 2023 Terlević et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Article
Terlević, Robert
Ulamec, Monika
Štimac, Goran
Murgić, Jure
Krušlin, Božo
Molecular classification of muscle-invasive bladder cancer based on a simplified immunohistochemical panel using GATA3, CK5/6, and p16
title Molecular classification of muscle-invasive bladder cancer based on a simplified immunohistochemical panel using GATA3, CK5/6, and p16
title_full Molecular classification of muscle-invasive bladder cancer based on a simplified immunohistochemical panel using GATA3, CK5/6, and p16
title_fullStr Molecular classification of muscle-invasive bladder cancer based on a simplified immunohistochemical panel using GATA3, CK5/6, and p16
title_full_unstemmed Molecular classification of muscle-invasive bladder cancer based on a simplified immunohistochemical panel using GATA3, CK5/6, and p16
title_short Molecular classification of muscle-invasive bladder cancer based on a simplified immunohistochemical panel using GATA3, CK5/6, and p16
title_sort molecular classification of muscle-invasive bladder cancer based on a simplified immunohistochemical panel using gata3, ck5/6, and p16
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10655881/
https://www.ncbi.nlm.nih.gov/pubmed/37389960
http://dx.doi.org/10.17305/bb.2023.9242
work_keys_str_mv AT terlevicrobert molecularclassificationofmuscleinvasivebladdercancerbasedonasimplifiedimmunohistochemicalpanelusinggata3ck56andp16
AT ulamecmonika molecularclassificationofmuscleinvasivebladdercancerbasedonasimplifiedimmunohistochemicalpanelusinggata3ck56andp16
AT stimacgoran molecularclassificationofmuscleinvasivebladdercancerbasedonasimplifiedimmunohistochemicalpanelusinggata3ck56andp16
AT murgicjure molecularclassificationofmuscleinvasivebladdercancerbasedonasimplifiedimmunohistochemicalpanelusinggata3ck56andp16
AT kruslinbozo molecularclassificationofmuscleinvasivebladdercancerbasedonasimplifiedimmunohistochemicalpanelusinggata3ck56andp16