Protocol for the in vitro isolation and culture of mature adipocytes and white adipose tissue explants from humans and mice

White adipose tissue (WAT) explants culture allows the study of this tissue ex vivo, maintaining its structure and properties. Concurrently, isolating mature adipocytes facilitates research into fat cell metabolism and hormonal regulation. Here, we present a protocol for obtaining, isolating, and pr...

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Autores principales: Villanueva-Carmona, Teresa, Cedó, Lídia, Núñez-Roa, Catalina, Maymó-Masip, Elsa, Vendrell, Joan, Fernández-Veledo, Sonia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656257/
https://www.ncbi.nlm.nih.gov/pubmed/37924518
http://dx.doi.org/10.1016/j.xpro.2023.102693
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author Villanueva-Carmona, Teresa
Cedó, Lídia
Núñez-Roa, Catalina
Maymó-Masip, Elsa
Vendrell, Joan
Fernández-Veledo, Sonia
author_facet Villanueva-Carmona, Teresa
Cedó, Lídia
Núñez-Roa, Catalina
Maymó-Masip, Elsa
Vendrell, Joan
Fernández-Veledo, Sonia
author_sort Villanueva-Carmona, Teresa
collection PubMed
description White adipose tissue (WAT) explants culture allows the study of this tissue ex vivo, maintaining its structure and properties. Concurrently, isolating mature adipocytes facilitates research into fat cell metabolism and hormonal regulation. Here, we present a protocol for obtaining, isolating, and processing mature adipocytes, alongside the cultivation of WAT explants from humans and mice. We describe steps for WAT retrieval, culturing of WAT explants, WAT digestion, and adipocytes separation. We then detail procedures for culturing isolated mature adipocytes. For complete details on the use and execution of this protocol, please refer to Villanueva-Carmona et al. (2023).(1)
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spelling pubmed-106562572023-11-03 Protocol for the in vitro isolation and culture of mature adipocytes and white adipose tissue explants from humans and mice Villanueva-Carmona, Teresa Cedó, Lídia Núñez-Roa, Catalina Maymó-Masip, Elsa Vendrell, Joan Fernández-Veledo, Sonia STAR Protoc Protocol White adipose tissue (WAT) explants culture allows the study of this tissue ex vivo, maintaining its structure and properties. Concurrently, isolating mature adipocytes facilitates research into fat cell metabolism and hormonal regulation. Here, we present a protocol for obtaining, isolating, and processing mature adipocytes, alongside the cultivation of WAT explants from humans and mice. We describe steps for WAT retrieval, culturing of WAT explants, WAT digestion, and adipocytes separation. We then detail procedures for culturing isolated mature adipocytes. For complete details on the use and execution of this protocol, please refer to Villanueva-Carmona et al. (2023).(1) Elsevier 2023-11-03 /pmc/articles/PMC10656257/ /pubmed/37924518 http://dx.doi.org/10.1016/j.xpro.2023.102693 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Villanueva-Carmona, Teresa
Cedó, Lídia
Núñez-Roa, Catalina
Maymó-Masip, Elsa
Vendrell, Joan
Fernández-Veledo, Sonia
Protocol for the in vitro isolation and culture of mature adipocytes and white adipose tissue explants from humans and mice
title Protocol for the in vitro isolation and culture of mature adipocytes and white adipose tissue explants from humans and mice
title_full Protocol for the in vitro isolation and culture of mature adipocytes and white adipose tissue explants from humans and mice
title_fullStr Protocol for the in vitro isolation and culture of mature adipocytes and white adipose tissue explants from humans and mice
title_full_unstemmed Protocol for the in vitro isolation and culture of mature adipocytes and white adipose tissue explants from humans and mice
title_short Protocol for the in vitro isolation and culture of mature adipocytes and white adipose tissue explants from humans and mice
title_sort protocol for the in vitro isolation and culture of mature adipocytes and white adipose tissue explants from humans and mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656257/
https://www.ncbi.nlm.nih.gov/pubmed/37924518
http://dx.doi.org/10.1016/j.xpro.2023.102693
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