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Protocol for the in vitro isolation and culture of mature adipocytes and white adipose tissue explants from humans and mice

White adipose tissue (WAT) explants culture allows the study of this tissue ex vivo, maintaining its structure and properties. Concurrently, isolating mature adipocytes facilitates research into fat cell metabolism and hormonal regulation. Here, we present a protocol for obtaining, isolating, and pr...

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Detalles Bibliográficos
Autores principales: Villanueva-Carmona, Teresa, Cedó, Lídia, Núñez-Roa, Catalina, Maymó-Masip, Elsa, Vendrell, Joan, Fernández-Veledo, Sonia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656257/
https://www.ncbi.nlm.nih.gov/pubmed/37924518
http://dx.doi.org/10.1016/j.xpro.2023.102693