A protocol for selection in mice of highly metastatic ovarian cancer cell with omental tropism

Preclinical models mimicking spontaneous omental metastasis from ovarian cancer (OC) can benefit the study of anti-metastatic therapies for OC patients. Here, we present a protocol to establish a highly metastatic (HM) mouse model with omental tropism by in vivo selection. We describe the processes...

Descripción completa

Detalles Bibliográficos
Autores principales: Guo, Jing, Ying, Feiquan, Cai, Jing, Wang, Zehua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656258/
https://www.ncbi.nlm.nih.gov/pubmed/37924519
http://dx.doi.org/10.1016/j.xpro.2023.102642
_version_ 1785136974747664384
author Guo, Jing
Ying, Feiquan
Cai, Jing
Wang, Zehua
author_facet Guo, Jing
Ying, Feiquan
Cai, Jing
Wang, Zehua
author_sort Guo, Jing
collection PubMed
description Preclinical models mimicking spontaneous omental metastasis from ovarian cancer (OC) can benefit the study of anti-metastatic therapies for OC patients. Here, we present a protocol to establish a highly metastatic (HM) mouse model with omental tropism by in vivo selection. We describe the processes of implanting OC cells in the ovaries of mice and obtaining HM sublines from their omental metastases. HM cells can metastasize from the ovary to the omentum within 2 weeks. For complete details on the use and execution of this protocol, please refer to Ying et al.(1)
format Online
Article
Text
id pubmed-10656258
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-106562582023-11-03 A protocol for selection in mice of highly metastatic ovarian cancer cell with omental tropism Guo, Jing Ying, Feiquan Cai, Jing Wang, Zehua STAR Protoc Protocol Preclinical models mimicking spontaneous omental metastasis from ovarian cancer (OC) can benefit the study of anti-metastatic therapies for OC patients. Here, we present a protocol to establish a highly metastatic (HM) mouse model with omental tropism by in vivo selection. We describe the processes of implanting OC cells in the ovaries of mice and obtaining HM sublines from their omental metastases. HM cells can metastasize from the ovary to the omentum within 2 weeks. For complete details on the use and execution of this protocol, please refer to Ying et al.(1) Elsevier 2023-11-03 /pmc/articles/PMC10656258/ /pubmed/37924519 http://dx.doi.org/10.1016/j.xpro.2023.102642 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Guo, Jing
Ying, Feiquan
Cai, Jing
Wang, Zehua
A protocol for selection in mice of highly metastatic ovarian cancer cell with omental tropism
title A protocol for selection in mice of highly metastatic ovarian cancer cell with omental tropism
title_full A protocol for selection in mice of highly metastatic ovarian cancer cell with omental tropism
title_fullStr A protocol for selection in mice of highly metastatic ovarian cancer cell with omental tropism
title_full_unstemmed A protocol for selection in mice of highly metastatic ovarian cancer cell with omental tropism
title_short A protocol for selection in mice of highly metastatic ovarian cancer cell with omental tropism
title_sort protocol for selection in mice of highly metastatic ovarian cancer cell with omental tropism
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656258/
https://www.ncbi.nlm.nih.gov/pubmed/37924519
http://dx.doi.org/10.1016/j.xpro.2023.102642
work_keys_str_mv AT guojing aprotocolforselectioninmiceofhighlymetastaticovariancancercellwithomentaltropism
AT yingfeiquan aprotocolforselectioninmiceofhighlymetastaticovariancancercellwithomentaltropism
AT caijing aprotocolforselectioninmiceofhighlymetastaticovariancancercellwithomentaltropism
AT wangzehua aprotocolforselectioninmiceofhighlymetastaticovariancancercellwithomentaltropism
AT guojing protocolforselectioninmiceofhighlymetastaticovariancancercellwithomentaltropism
AT yingfeiquan protocolforselectioninmiceofhighlymetastaticovariancancercellwithomentaltropism
AT caijing protocolforselectioninmiceofhighlymetastaticovariancancercellwithomentaltropism
AT wangzehua protocolforselectioninmiceofhighlymetastaticovariancancercellwithomentaltropism

Ejemplares similares