Cargando…

Enzyme-less nanopore detection of post-translational modifications within long polypeptides

Means to analyse cellular proteins and their millions of variants at the single-molecule level would uncover substantial information previously unknown to biology. Nanopore technology, which underpins long-read DNA and RNA sequencing, holds potential for full-length proteoform identification. We use...

Descripción completa

Detalles Bibliográficos
Autores principales:	Martin-Baniandres, Pablo, Lan, Wei-Hsuan, Board, Stephanie, Romero-Ruiz, Mercedes, Garcia-Manyes, Sergi, Qing, Yujia, Bayley, Hagan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656283/ https://www.ncbi.nlm.nih.gov/pubmed/37500774 http://dx.doi.org/10.1038/s41565-023-01462-8

Ejemplares similares

High-throughput optical sensing of nucleic acids in a nanopore array
por: Huang, Shuo, et al.
Publicado: (2015)

Protein Detection by Nanopores Equipped with Aptamers
por: Rotem, Dvir, et al.
Publicado: (2012)

Transmembrane protein rotaxanes reveal kinetic traps in the refolding of translocated substrates
por: Feng, Jianfei, et al.
Publicado: (2020)

Label-Free Detection of Post-translational Modifications with a Nanopore
por: Restrepo-Pérez, Laura, et al.
Publicado: (2019)

Nanopore Detection Using Supercharged Polypeptide Molecular Carriers
por: Wang, Xiaoyi, et al.
Publicado: (2023)

Catalytic site-selective substrate processing within a tubular nanoreactor
por: Qing, Yujia, et al.
Publicado: (2019)

Identification of a novel mammalian post-translational modification, phosphocholine, on placental secretory polypeptides
por: Lovell, Tristan M, et al.
Publicado: (2007)

DNA cleavage and methylation specificity of the single polypeptide restriction–modification enzyme LlaGI
por: Smith, Rachel M., et al.
Publicado: (2009)

An Mrr-family nuclease motif in the single polypeptide restriction–modification enzyme LlaGI
por: Smith, Rachel M., et al.
Publicado: (2009)

Nanopore Discrimination of Coagulation Biomarker Derivatives and Characterization of a Post-Translational Modification
por: Stierlen, Aïcha, et al.
Publicado: (2023)

Regulation of Deubiquitinating Enzymes by Post-Translational Modifications
por: Das, Tanuza, et al.
Publicado: (2020)

Modification mapping by nanopore sequencing
por: White, Laura K., et al.
Publicado: (2022)

A Single-Molecule Strategy to Capture Non-native Intramolecular and Intermolecular Protein Disulfide Bridges
por: Mora, Marc, et al.
Publicado: (2022)

Functionalised nanopores: chemical and biological modifications
por: Cairns-Gibson, Dominic F., et al.
Publicado: (2021)

Molecular fluctuations as a ruler of force-induced protein conformations
por: Stannard, Andrew, et al.
Publicado: (2021)

Post-Translational Modifications of Deubiquitinating Enzymes: Expanding the Ubiquitin Code
por: Wang, Yanfeng, et al.
Publicado: (2021)

Prolonged Residence Time of a Noncovalent Molecular Adapter, β-Cyclodextrin, within the Lumen of Mutant α-Hemolysin Pores
por: Gu, Li-Qun, et al.
Publicado: (2001)

The single polypeptide restriction–modification enzyme LlaGI is a self-contained molecular motor that translocates DNA loops
por: Smith, Rachel M., et al.
Publicado: (2009)

Enhanced statistical sampling reveals microscopic complexity in the talin mechanosensor folding energy landscape
por: Tapia-Rojo, Rafael, et al.
Publicado: (2023)

Enzyme-mimetic self-catalyzed polymerization of polypeptide helices
por: Song, Ziyuan, et al.
Publicado: (2019)

Nanopore-Based Detection of Viral RNA Modifications
por: Abebe, Jonathan S., et al.
Publicado: (2022)

CONTROLLED PROTEOLYSIS OF NASCENT POLYPEPTIDES IN RAT LIVER CELL FRACTIONS : I. Location of the Polypeptides within Ribosomes
por: Blobel, G., et al.
Publicado: (1970)

Organization of mammalian neurofilament polypeptides within the neuronal cytoskeleton
Publicado: (1984)

Resolving Chemical Modifications to a Single Amino Acid within a Peptide Using a Biological Nanopore
por: Restrepo-Pérez, Laura, et al.
Publicado: (2019)

Post-translational modification enzymes as key regulators of ciliary protein trafficking
por: Chaya, Taro, et al.
Publicado: (2021)

Enzyme Immobilization on Nanoporous Gold: A Review
por: Stine, Keith J
Publicado: (2017)

Co-translational mechanisms of quality control of newly synthesized polypeptides
por: Gandin, Valentina, et al.
Publicado: (2014)

Application of enzymes in the preparation of wheat germ polypeptides and their biological activities
por: Du, Ke, et al.
Publicado: (2022)

PTMcode v2: a resource for functional associations of post-translational modifications within and between proteins
por: Minguez, Pablo, et al.
Publicado: (2014)

Correction to Resolving Chemical Modifications to a Single Amino Acid within a Peptide Using a Biological Nanopore
por: Restrepo-Pérez, Laura, et al.
Publicado: (2020)

novPTMenzy: a database for enzymes involved in novel post-translational modifications
por: Khater, Shradha, et al.
Publicado: (2015)

Redox-Mediated Post-Translational Modifications of Proteolytic Enzymes and Their Role in Protease Functioning
por: Petushkova, Anastasiia I., et al.
Publicado: (2020)

The role of single protein elasticity in mechanobiology
por: Beedle, Amy EM, et al.
Publicado: (2023)

Less translational control, more memory
por: Pavitt, Graham D
Publicado: (2013)

Protection of Catalytic Cofactors by Polypeptides as a Driver for the Emergence of Primordial Enzymes
por: Gutierrez-Rus, Luis I, et al.
Publicado: (2023)

Surface Modification of a Nanoporous Carbon Photoanode upon Irradiation
por: Gomis-Berenguer, Alicia, et al.
Publicado: (2016)

Recent advances in the detection of base modifications using the Nanopore sequencer
por: Xu, Liu, et al.
Publicado: (2019)

RNA modifications detection by comparative Nanopore direct RNA sequencing
por: Leger, Adrien, et al.
Publicado: (2021)

Multilevel Regulation of Peroxisomal Proteome by Post-Translational Modifications
por: Sandalio, Luisa M., et al.
Publicado: (2019)

Single-molecule paleoenzymology probes the chemistry of resurrected enzymes
por: Perez-Jimenez, Raul, et al.
Publicado: (2011)

Cannot write session to /tmp/vufind_sessions/sess_kvgcsulbjq3pjru5clc946u3pv