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Polyphosphate-loaded silk fibroin membrane as hemostatic agent in oral surgery: a pilot study
PURPOSE: Post-interventional hemorrhage can result in serious complications, especially in patients with hemostatic disorders. Identification of safe and efficient local hemostatic agents is important, particularly in the context of an ageing society and the emergence of new oral anticoagulants. The...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656390/ https://www.ncbi.nlm.nih.gov/pubmed/37975954 http://dx.doi.org/10.1186/s40729-023-00503-0 |
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author | Popal, Zohal Nickel, Katrin F. Wöltje, Michael Aibibu, Dilbar Knipfer, Christian Smeets, Ralf Renné, Thomas |
author_facet | Popal, Zohal Nickel, Katrin F. Wöltje, Michael Aibibu, Dilbar Knipfer, Christian Smeets, Ralf Renné, Thomas |
author_sort | Popal, Zohal |
collection | PubMed |
description | PURPOSE: Post-interventional hemorrhage can result in serious complications, especially in patients with hemostatic disorders. Identification of safe and efficient local hemostatic agents is important, particularly in the context of an ageing society and the emergence of new oral anticoagulants. The aim of this in vitro study was to investigate the potential of silk fibroin membranes coated with the inorganic polymer polyphosphate (polyP) as a novel hemostatic device in oral surgery. METHODS: Cocoons of the silkworm Bombyx mori were degummed and dissolved. Varying amounts of long-chain polyP (2–2000 µg/mm(2)) were adsorbed to the surface of silk fibroin membranes. Analysis of the procoagulant effect of polyP-coated silk membranes was performed using real-time thrombin generation assays in human plasma. Increasing concentrations of polyP (0.15–500 µg/ml) served as a positive control, while uncoated silk fibroin membranes were used as negative control. RESULTS: PolyP-coated silk fibroin membranes triggered coagulation when compared to plasma samples and pure silk fibroin membranes. A polyP-dose-dependent effect of thrombin generation could be found with a maximum (ETP = 1525.7 nM⋅min, peak thrombin = 310.1 nM, time to peak = 9.8 min, lag time = 7.6 min.) at 200 µg/mm(2) of polymer loading on the silk fibroin membrane surface. CONCLUSIONS: In this study, it was demonstrated that silk fibroin membranes coated with polyP have the potential to act as a promising novel hemostatic device. GRAPHICAL ABSTRACT: [Image: see text] |
format | Online Article Text |
id | pubmed-10656390 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-106563902023-11-17 Polyphosphate-loaded silk fibroin membrane as hemostatic agent in oral surgery: a pilot study Popal, Zohal Nickel, Katrin F. Wöltje, Michael Aibibu, Dilbar Knipfer, Christian Smeets, Ralf Renné, Thomas Int J Implant Dent Research PURPOSE: Post-interventional hemorrhage can result in serious complications, especially in patients with hemostatic disorders. Identification of safe and efficient local hemostatic agents is important, particularly in the context of an ageing society and the emergence of new oral anticoagulants. The aim of this in vitro study was to investigate the potential of silk fibroin membranes coated with the inorganic polymer polyphosphate (polyP) as a novel hemostatic device in oral surgery. METHODS: Cocoons of the silkworm Bombyx mori were degummed and dissolved. Varying amounts of long-chain polyP (2–2000 µg/mm(2)) were adsorbed to the surface of silk fibroin membranes. Analysis of the procoagulant effect of polyP-coated silk membranes was performed using real-time thrombin generation assays in human plasma. Increasing concentrations of polyP (0.15–500 µg/ml) served as a positive control, while uncoated silk fibroin membranes were used as negative control. RESULTS: PolyP-coated silk fibroin membranes triggered coagulation when compared to plasma samples and pure silk fibroin membranes. A polyP-dose-dependent effect of thrombin generation could be found with a maximum (ETP = 1525.7 nM⋅min, peak thrombin = 310.1 nM, time to peak = 9.8 min, lag time = 7.6 min.) at 200 µg/mm(2) of polymer loading on the silk fibroin membrane surface. CONCLUSIONS: In this study, it was demonstrated that silk fibroin membranes coated with polyP have the potential to act as a promising novel hemostatic device. GRAPHICAL ABSTRACT: [Image: see text] Springer Berlin Heidelberg 2023-11-17 /pmc/articles/PMC10656390/ /pubmed/37975954 http://dx.doi.org/10.1186/s40729-023-00503-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Popal, Zohal Nickel, Katrin F. Wöltje, Michael Aibibu, Dilbar Knipfer, Christian Smeets, Ralf Renné, Thomas Polyphosphate-loaded silk fibroin membrane as hemostatic agent in oral surgery: a pilot study |
title | Polyphosphate-loaded silk fibroin membrane as hemostatic agent in oral surgery: a pilot study |
title_full | Polyphosphate-loaded silk fibroin membrane as hemostatic agent in oral surgery: a pilot study |
title_fullStr | Polyphosphate-loaded silk fibroin membrane as hemostatic agent in oral surgery: a pilot study |
title_full_unstemmed | Polyphosphate-loaded silk fibroin membrane as hemostatic agent in oral surgery: a pilot study |
title_short | Polyphosphate-loaded silk fibroin membrane as hemostatic agent in oral surgery: a pilot study |
title_sort | polyphosphate-loaded silk fibroin membrane as hemostatic agent in oral surgery: a pilot study |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656390/ https://www.ncbi.nlm.nih.gov/pubmed/37975954 http://dx.doi.org/10.1186/s40729-023-00503-0 |
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