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Construction of ultrasonically treated collagen/silk fibroin composite scaffolds to induce cartilage regeneration
A novel tissue-specific functional tissue engineering scaffold for cartilage repair should have a three-dimensional structure, good biosafety and biological activity, and should be able to promote cartilage tissue regeneration. This study aimed to determine the effect of ultrasound-treated collagen/...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656553/ https://www.ncbi.nlm.nih.gov/pubmed/37978248 http://dx.doi.org/10.1038/s41598-023-43397-z |
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author | Yu, Shunan Shu, Xiong Chen, Lei Wang, Chao Wang, Xinyu Jing, Jinzhu Yan, Guoqiang Zhang, Yanzhuo Wu, Chengai |
author_facet | Yu, Shunan Shu, Xiong Chen, Lei Wang, Chao Wang, Xinyu Jing, Jinzhu Yan, Guoqiang Zhang, Yanzhuo Wu, Chengai |
author_sort | Yu, Shunan |
collection | PubMed |
description | A novel tissue-specific functional tissue engineering scaffold for cartilage repair should have a three-dimensional structure, good biosafety and biological activity, and should be able to promote cartilage tissue regeneration. This study aimed to determine the effect of ultrasound-treated collagen/silk fibroin (Col/SF) composite scaffolds with good mechanical properties and high biological activity on cartilage repair. The characteristics of the scaffolds with different Col/SF ratios (7:3, 8:2, and 9:1) were determined by scanning electron microscopy, Fourier-transform infrared spectroscopy, and porosity, water absorption, and compression tests. In vitro evaluations revealed the biocompatibility of the Col/SF scaffolds. Results suggested that the optimal ratio of Col/SF composite scaffolds was 7:3. The Col/SF scaffolds induced adipose-derived stem cells to undergo chondrogenic differentiation under chondrogenic culture conditions. The efficiency of Col/SF scaffolds for cartilage regeneration applications was further evaluated using an in vivo model of full-thickness articular cartilage defects in New Zealand rabbits. The Col/SF scaffolds effectively promoted osteochondral regeneration as evidenced by macroscopic, histological, and immunohistochemical evaluation. The study demonstrates that ultrasound-treated Col/SF scaffolds show great potential for repairing cartilage defects. |
format | Online Article Text |
id | pubmed-10656553 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-106565532023-11-17 Construction of ultrasonically treated collagen/silk fibroin composite scaffolds to induce cartilage regeneration Yu, Shunan Shu, Xiong Chen, Lei Wang, Chao Wang, Xinyu Jing, Jinzhu Yan, Guoqiang Zhang, Yanzhuo Wu, Chengai Sci Rep Article A novel tissue-specific functional tissue engineering scaffold for cartilage repair should have a three-dimensional structure, good biosafety and biological activity, and should be able to promote cartilage tissue regeneration. This study aimed to determine the effect of ultrasound-treated collagen/silk fibroin (Col/SF) composite scaffolds with good mechanical properties and high biological activity on cartilage repair. The characteristics of the scaffolds with different Col/SF ratios (7:3, 8:2, and 9:1) were determined by scanning electron microscopy, Fourier-transform infrared spectroscopy, and porosity, water absorption, and compression tests. In vitro evaluations revealed the biocompatibility of the Col/SF scaffolds. Results suggested that the optimal ratio of Col/SF composite scaffolds was 7:3. The Col/SF scaffolds induced adipose-derived stem cells to undergo chondrogenic differentiation under chondrogenic culture conditions. The efficiency of Col/SF scaffolds for cartilage regeneration applications was further evaluated using an in vivo model of full-thickness articular cartilage defects in New Zealand rabbits. The Col/SF scaffolds effectively promoted osteochondral regeneration as evidenced by macroscopic, histological, and immunohistochemical evaluation. The study demonstrates that ultrasound-treated Col/SF scaffolds show great potential for repairing cartilage defects. Nature Publishing Group UK 2023-11-17 /pmc/articles/PMC10656553/ /pubmed/37978248 http://dx.doi.org/10.1038/s41598-023-43397-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Yu, Shunan Shu, Xiong Chen, Lei Wang, Chao Wang, Xinyu Jing, Jinzhu Yan, Guoqiang Zhang, Yanzhuo Wu, Chengai Construction of ultrasonically treated collagen/silk fibroin composite scaffolds to induce cartilage regeneration |
title | Construction of ultrasonically treated collagen/silk fibroin composite scaffolds to induce cartilage regeneration |
title_full | Construction of ultrasonically treated collagen/silk fibroin composite scaffolds to induce cartilage regeneration |
title_fullStr | Construction of ultrasonically treated collagen/silk fibroin composite scaffolds to induce cartilage regeneration |
title_full_unstemmed | Construction of ultrasonically treated collagen/silk fibroin composite scaffolds to induce cartilage regeneration |
title_short | Construction of ultrasonically treated collagen/silk fibroin composite scaffolds to induce cartilage regeneration |
title_sort | construction of ultrasonically treated collagen/silk fibroin composite scaffolds to induce cartilage regeneration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656553/ https://www.ncbi.nlm.nih.gov/pubmed/37978248 http://dx.doi.org/10.1038/s41598-023-43397-z |
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