Changing the phospholipid composition of lipid droplets alters localization of select lipid droplet proteins

Our experiments aim to determine if decreasing the amount of phosphatidylcholine (PC) relative to phosphatidylethanolamine (PE) at the lipid droplet surface changes the localization of specific lipid droplet proteins. We manipulate lipid droplet phospholipids in both a cultured mouse hepatocyte (AML...

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Detalles Bibliográficos
Autores principales: Renier, Timothy J., Paetz, Olivia R., Paal, Matthew C., Long, Alex B., Brown, Margaret R., Vuong, Sunny H., Perumal, Sathish Kumar, Kharbanda, Kusum K., Listenberger, Laura L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656625/
https://www.ncbi.nlm.nih.gov/pubmed/38021172
http://dx.doi.org/10.17912/micropub.biology.000960
Descripción
Sumario:Our experiments aim to determine if decreasing the amount of phosphatidylcholine (PC) relative to phosphatidylethanolamine (PE) at the lipid droplet surface changes the localization of specific lipid droplet proteins. We manipulate lipid droplet phospholipids in both a cultured mouse hepatocyte (AML12) cell line and on synthetic lipid droplets. Decreasing the PC:PE ratio increases perilipin 2, decreases DGAT2, and does not change rab18 or lanosterol synthase levels on lipid droplets. These differences may be explained by the distinct structural motifs that mediate the protein-lipid droplet interactions.

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