Changing the phospholipid composition of lipid droplets alters localization of select lipid droplet proteins

Our experiments aim to determine if decreasing the amount of phosphatidylcholine (PC) relative to phosphatidylethanolamine (PE) at the lipid droplet surface changes the localization of specific lipid droplet proteins. We manipulate lipid droplet phospholipids in both a cultured mouse hepatocyte (AML...

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Autores principales: Renier, Timothy J., Paetz, Olivia R., Paal, Matthew C., Long, Alex B., Brown, Margaret R., Vuong, Sunny H., Perumal, Sathish Kumar, Kharbanda, Kusum K., Listenberger, Laura L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656625/
https://www.ncbi.nlm.nih.gov/pubmed/38021172
http://dx.doi.org/10.17912/micropub.biology.000960
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author Renier, Timothy J.
Paetz, Olivia R.
Paal, Matthew C.
Long, Alex B.
Brown, Margaret R.
Vuong, Sunny H.
Perumal, Sathish Kumar
Kharbanda, Kusum K.
Listenberger, Laura L.
author_facet Renier, Timothy J.
Paetz, Olivia R.
Paal, Matthew C.
Long, Alex B.
Brown, Margaret R.
Vuong, Sunny H.
Perumal, Sathish Kumar
Kharbanda, Kusum K.
Listenberger, Laura L.
author_sort Renier, Timothy J.
collection PubMed
description Our experiments aim to determine if decreasing the amount of phosphatidylcholine (PC) relative to phosphatidylethanolamine (PE) at the lipid droplet surface changes the localization of specific lipid droplet proteins. We manipulate lipid droplet phospholipids in both a cultured mouse hepatocyte (AML12) cell line and on synthetic lipid droplets. Decreasing the PC:PE ratio increases perilipin 2, decreases DGAT2, and does not change rab18 or lanosterol synthase levels on lipid droplets. These differences may be explained by the distinct structural motifs that mediate the protein-lipid droplet interactions.
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spelling pubmed-106566252023-11-03 Changing the phospholipid composition of lipid droplets alters localization of select lipid droplet proteins Renier, Timothy J. Paetz, Olivia R. Paal, Matthew C. Long, Alex B. Brown, Margaret R. Vuong, Sunny H. Perumal, Sathish Kumar Kharbanda, Kusum K. Listenberger, Laura L. MicroPubl Biol New Finding Our experiments aim to determine if decreasing the amount of phosphatidylcholine (PC) relative to phosphatidylethanolamine (PE) at the lipid droplet surface changes the localization of specific lipid droplet proteins. We manipulate lipid droplet phospholipids in both a cultured mouse hepatocyte (AML12) cell line and on synthetic lipid droplets. Decreasing the PC:PE ratio increases perilipin 2, decreases DGAT2, and does not change rab18 or lanosterol synthase levels on lipid droplets. These differences may be explained by the distinct structural motifs that mediate the protein-lipid droplet interactions. Caltech Library 2023-11-03 /pmc/articles/PMC10656625/ /pubmed/38021172 http://dx.doi.org/10.17912/micropub.biology.000960 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Renier, Timothy J.
Paetz, Olivia R.
Paal, Matthew C.
Long, Alex B.
Brown, Margaret R.
Vuong, Sunny H.
Perumal, Sathish Kumar
Kharbanda, Kusum K.
Listenberger, Laura L.
Changing the phospholipid composition of lipid droplets alters localization of select lipid droplet proteins
title Changing the phospholipid composition of lipid droplets alters localization of select lipid droplet proteins
title_full Changing the phospholipid composition of lipid droplets alters localization of select lipid droplet proteins
title_fullStr Changing the phospholipid composition of lipid droplets alters localization of select lipid droplet proteins
title_full_unstemmed Changing the phospholipid composition of lipid droplets alters localization of select lipid droplet proteins
title_short Changing the phospholipid composition of lipid droplets alters localization of select lipid droplet proteins
title_sort changing the phospholipid composition of lipid droplets alters localization of select lipid droplet proteins
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656625/
https://www.ncbi.nlm.nih.gov/pubmed/38021172
http://dx.doi.org/10.17912/micropub.biology.000960
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