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lncRNA LENGA sponges miR-378 to promote myocardial fibrosis in atrial fibrillation

miR-378 is known to suppress myocardial fibrosis, while its upstream regulators have not been identified. lncRNA LENGA is a recently identified lncRNA in cancer biology. We observed the altered expression of LENGA in atrial fibrillation (AF) patients and predicted its interaction with miR-378. We th...

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Autores principales: Wu, Liting, Gao, Bingjing, Shen, Mengyuan, Wei, Lu, Li, Zhumeng, Zhuang, Wenfang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: De Gruyter 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656758/
https://www.ncbi.nlm.nih.gov/pubmed/38025533
http://dx.doi.org/10.1515/med-2023-0831
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author Wu, Liting
Gao, Bingjing
Shen, Mengyuan
Wei, Lu
Li, Zhumeng
Zhuang, Wenfang
author_facet Wu, Liting
Gao, Bingjing
Shen, Mengyuan
Wei, Lu
Li, Zhumeng
Zhuang, Wenfang
author_sort Wu, Liting
collection PubMed
description miR-378 is known to suppress myocardial fibrosis, while its upstream regulators have not been identified. lncRNA LENGA is a recently identified lncRNA in cancer biology. We observed the altered expression of LENGA in atrial fibrillation (AF) patients and predicted its interaction with miR-378. We then explored the interaction between LENGA and miR-378 in AF. Angiotensin-II (Ang-II)-induced human atrial cardiac fibroblasts and human atrial muscle tissues were collected and the expression of LENGA and miR-378 was determined by RT-qPCR. The interaction between LENGA and miR-378 was analyzed through bioinformatics analysis and confirmed by RNA pulldown assay. Cell proliferation and collagen production were analyzed through in vitro assay to analyze the role of LENGA and miR-378 in MF. AF patients showed increased expression of LENGA and deceased expression of miR-378 compared to the sinus rhythm group. LENGA and miR-378 interacted with each other, while they are not closely correlated with each other. Overexpression assay showed that LENGA and miR-378 overexpression failed to affect each other’s expression. LENGA promoted collagen production and proliferation of Ang-II-induced atrial fibroblasts, while miR-378 played opposite roles. Moreover, LENGA suppressed the function of miR-378. Therefore, LENGA may sponge miR-378 to promote MF in AF.
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spelling pubmed-106567582023-11-14 lncRNA LENGA sponges miR-378 to promote myocardial fibrosis in atrial fibrillation Wu, Liting Gao, Bingjing Shen, Mengyuan Wei, Lu Li, Zhumeng Zhuang, Wenfang Open Med (Wars) Research Article miR-378 is known to suppress myocardial fibrosis, while its upstream regulators have not been identified. lncRNA LENGA is a recently identified lncRNA in cancer biology. We observed the altered expression of LENGA in atrial fibrillation (AF) patients and predicted its interaction with miR-378. We then explored the interaction between LENGA and miR-378 in AF. Angiotensin-II (Ang-II)-induced human atrial cardiac fibroblasts and human atrial muscle tissues were collected and the expression of LENGA and miR-378 was determined by RT-qPCR. The interaction between LENGA and miR-378 was analyzed through bioinformatics analysis and confirmed by RNA pulldown assay. Cell proliferation and collagen production were analyzed through in vitro assay to analyze the role of LENGA and miR-378 in MF. AF patients showed increased expression of LENGA and deceased expression of miR-378 compared to the sinus rhythm group. LENGA and miR-378 interacted with each other, while they are not closely correlated with each other. Overexpression assay showed that LENGA and miR-378 overexpression failed to affect each other’s expression. LENGA promoted collagen production and proliferation of Ang-II-induced atrial fibroblasts, while miR-378 played opposite roles. Moreover, LENGA suppressed the function of miR-378. Therefore, LENGA may sponge miR-378 to promote MF in AF. De Gruyter 2023-11-14 /pmc/articles/PMC10656758/ /pubmed/38025533 http://dx.doi.org/10.1515/med-2023-0831 Text en © 2023 the author(s), published by De Gruyter https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License.
spellingShingle Research Article
Wu, Liting
Gao, Bingjing
Shen, Mengyuan
Wei, Lu
Li, Zhumeng
Zhuang, Wenfang
lncRNA LENGA sponges miR-378 to promote myocardial fibrosis in atrial fibrillation
title lncRNA LENGA sponges miR-378 to promote myocardial fibrosis in atrial fibrillation
title_full lncRNA LENGA sponges miR-378 to promote myocardial fibrosis in atrial fibrillation
title_fullStr lncRNA LENGA sponges miR-378 to promote myocardial fibrosis in atrial fibrillation
title_full_unstemmed lncRNA LENGA sponges miR-378 to promote myocardial fibrosis in atrial fibrillation
title_short lncRNA LENGA sponges miR-378 to promote myocardial fibrosis in atrial fibrillation
title_sort lncrna lenga sponges mir-378 to promote myocardial fibrosis in atrial fibrillation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656758/
https://www.ncbi.nlm.nih.gov/pubmed/38025533
http://dx.doi.org/10.1515/med-2023-0831
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