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High variability of perezone content in rhizomes of Acourtia cordata wild plants, environmental factors related, and proteomic analysis

With the aim of exploring the source of the high variability observed in the production of perezone, in Acourtia cordata wild plants, we analyze the influence of soil parameters and phenotypic characteristics on its perezone content. Perezone is a sesquiterpene quinone responsible for several pharma...

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Autores principales: García Méndez, Ma del Carmen, Encarnación-Guevara, Sergio, Martínez Batallar, Ángel Gabriel, Gómez-Caudillo, Leopoldo, Bru-Martínez, Roque, Martínez Márquez, Ascensión, Selles Marchart, Susana, Tovar-Sánchez, Efraín, Álvarez-Berber, Laura, Marquina Bahena, Silvia, Perea-Arango, Irene, Arellano-García, José de Jesús
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656900/
https://www.ncbi.nlm.nih.gov/pubmed/38025722
http://dx.doi.org/10.7717/peerj.16136
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author García Méndez, Ma del Carmen
Encarnación-Guevara, Sergio
Martínez Batallar, Ángel Gabriel
Gómez-Caudillo, Leopoldo
Bru-Martínez, Roque
Martínez Márquez, Ascensión
Selles Marchart, Susana
Tovar-Sánchez, Efraín
Álvarez-Berber, Laura
Marquina Bahena, Silvia
Perea-Arango, Irene
Arellano-García, José de Jesús
author_facet García Méndez, Ma del Carmen
Encarnación-Guevara, Sergio
Martínez Batallar, Ángel Gabriel
Gómez-Caudillo, Leopoldo
Bru-Martínez, Roque
Martínez Márquez, Ascensión
Selles Marchart, Susana
Tovar-Sánchez, Efraín
Álvarez-Berber, Laura
Marquina Bahena, Silvia
Perea-Arango, Irene
Arellano-García, José de Jesús
author_sort García Méndez, Ma del Carmen
collection PubMed
description With the aim of exploring the source of the high variability observed in the production of perezone, in Acourtia cordata wild plants, we analyze the influence of soil parameters and phenotypic characteristics on its perezone content. Perezone is a sesquiterpene quinone responsible for several pharmacological effects and the A. cordata plants are the natural source of this metabolite. The chemistry of perezone has been widely studied, however, no studies exist related to its production under natural conditions, nor to its biosynthesis and the environmental factors that affect the yield of this compound in wild plants. We also used a proteomic approach to detect differentially expressed proteins in wild plant rhizomes and compare the profiles of high vs. low perezone-producing plants. Our results show that in perezone-producing rhizomes, the presence of high concentrations of this compound could result from a positive response to the effects of some edaphic factors, such as total phosphorus (P(t)), total nitrogen (N(t)), ammonium (NH(4)), and organic matter (O. M.), but could also be due to a negative response to the soil pH value. Additionally, we identified 616 differentially expressed proteins between high and low perezone producers. According to the functional annotation of this comparison, the upregulated proteins were grouped in valine biosynthesis, breakdown of leucine and isoleucine, and secondary metabolism such as terpenoid biosynthesis. Downregulated proteins were grouped in basal metabolism processes, such as pyruvate and purine metabolism and glycolysis/gluconeogenesis. Our results suggest that soil parameters can impact the content of perezone in wild plants. Furthermore, we used proteomic resources to obtain data on the pathways expressed when A. cordata plants produce high and low concentrations of perezone. These data may be useful to further explore the possible relationship between perezone production and abiotic or biotic factors and the molecular mechanisms related to high and low perezone production.
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spelling pubmed-106569002023-11-15 High variability of perezone content in rhizomes of Acourtia cordata wild plants, environmental factors related, and proteomic analysis García Méndez, Ma del Carmen Encarnación-Guevara, Sergio Martínez Batallar, Ángel Gabriel Gómez-Caudillo, Leopoldo Bru-Martínez, Roque Martínez Márquez, Ascensión Selles Marchart, Susana Tovar-Sánchez, Efraín Álvarez-Berber, Laura Marquina Bahena, Silvia Perea-Arango, Irene Arellano-García, José de Jesús PeerJ Biochemistry With the aim of exploring the source of the high variability observed in the production of perezone, in Acourtia cordata wild plants, we analyze the influence of soil parameters and phenotypic characteristics on its perezone content. Perezone is a sesquiterpene quinone responsible for several pharmacological effects and the A. cordata plants are the natural source of this metabolite. The chemistry of perezone has been widely studied, however, no studies exist related to its production under natural conditions, nor to its biosynthesis and the environmental factors that affect the yield of this compound in wild plants. We also used a proteomic approach to detect differentially expressed proteins in wild plant rhizomes and compare the profiles of high vs. low perezone-producing plants. Our results show that in perezone-producing rhizomes, the presence of high concentrations of this compound could result from a positive response to the effects of some edaphic factors, such as total phosphorus (P(t)), total nitrogen (N(t)), ammonium (NH(4)), and organic matter (O. M.), but could also be due to a negative response to the soil pH value. Additionally, we identified 616 differentially expressed proteins between high and low perezone producers. According to the functional annotation of this comparison, the upregulated proteins were grouped in valine biosynthesis, breakdown of leucine and isoleucine, and secondary metabolism such as terpenoid biosynthesis. Downregulated proteins were grouped in basal metabolism processes, such as pyruvate and purine metabolism and glycolysis/gluconeogenesis. Our results suggest that soil parameters can impact the content of perezone in wild plants. Furthermore, we used proteomic resources to obtain data on the pathways expressed when A. cordata plants produce high and low concentrations of perezone. These data may be useful to further explore the possible relationship between perezone production and abiotic or biotic factors and the molecular mechanisms related to high and low perezone production. PeerJ Inc. 2023-11-15 /pmc/articles/PMC10656900/ /pubmed/38025722 http://dx.doi.org/10.7717/peerj.16136 Text en © 2023 Garcia Mendez et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Biochemistry
García Méndez, Ma del Carmen
Encarnación-Guevara, Sergio
Martínez Batallar, Ángel Gabriel
Gómez-Caudillo, Leopoldo
Bru-Martínez, Roque
Martínez Márquez, Ascensión
Selles Marchart, Susana
Tovar-Sánchez, Efraín
Álvarez-Berber, Laura
Marquina Bahena, Silvia
Perea-Arango, Irene
Arellano-García, José de Jesús
High variability of perezone content in rhizomes of Acourtia cordata wild plants, environmental factors related, and proteomic analysis
title High variability of perezone content in rhizomes of Acourtia cordata wild plants, environmental factors related, and proteomic analysis
title_full High variability of perezone content in rhizomes of Acourtia cordata wild plants, environmental factors related, and proteomic analysis
title_fullStr High variability of perezone content in rhizomes of Acourtia cordata wild plants, environmental factors related, and proteomic analysis
title_full_unstemmed High variability of perezone content in rhizomes of Acourtia cordata wild plants, environmental factors related, and proteomic analysis
title_short High variability of perezone content in rhizomes of Acourtia cordata wild plants, environmental factors related, and proteomic analysis
title_sort high variability of perezone content in rhizomes of acourtia cordata wild plants, environmental factors related, and proteomic analysis
topic Biochemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656900/
https://www.ncbi.nlm.nih.gov/pubmed/38025722
http://dx.doi.org/10.7717/peerj.16136
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