Intraplacental injection of AAV9-CMV-iCre results in the widespread transduction of multiple organs in double-reporter mouse embryos

Adeno-associated virus serotype 9 (AAV9) has become a popular tool for gene transfer because of its ability to cross the blood-brain barrier and efficiently transduce genetic material into a variety of cell types. The study utilized GRR (Green-to-Red Reporter) mouse embryos, in which the expression...

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Autores principales: Gogoleva, Natalia, Shahri, Zeynab Javanfekr, Noda, Atsushi, Liao, Ching-Wei, Wakimoto, Arata, Inoue, Yuri, Jeon, Hyojung, Takahashi, Satoru, Hamada, Michito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Association for Laboratory Animal Science 2023
Materias:
Original
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658086/
https://www.ncbi.nlm.nih.gov/pubmed/37183025
http://dx.doi.org/10.1538/expanim.23-0044
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author Gogoleva, Natalia
Shahri, Zeynab Javanfekr
Noda, Atsushi
Liao, Ching-Wei
Wakimoto, Arata
Inoue, Yuri
Jeon, Hyojung
Takahashi, Satoru
Hamada, Michito
author_facet Gogoleva, Natalia
Shahri, Zeynab Javanfekr
Noda, Atsushi
Liao, Ching-Wei
Wakimoto, Arata
Inoue, Yuri
Jeon, Hyojung
Takahashi, Satoru
Hamada, Michito
author_sort Gogoleva, Natalia
collection PubMed
description Adeno-associated virus serotype 9 (AAV9) has become a popular tool for gene transfer because of its ability to cross the blood-brain barrier and efficiently transduce genetic material into a variety of cell types. The study utilized GRR (Green-to-Red Reporter) mouse embryos, in which the expression of iCre results in the disappearance of Green Fluorescent Protein (GFP) expression and the detection of Discosoma sp. Red Fluorescent Protein (DsRed) expression by intraplacental injection. Our results demonstrate that AAV9-CMV-iCre can transduce multiple organs in embryos at developmental stages E9.5–E11.5, including the liver, heart, brain, thymus, and intestine. These findings suggest that intraplacental injection of AAV9-CMV-iCre is a viable method for the widespread transduction of GRR mouse embryos.
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spelling pubmed-106580862023-01-01 Intraplacental injection of AAV9-CMV-iCre results in the widespread transduction of multiple organs in double-reporter mouse embryos Gogoleva, Natalia Shahri, Zeynab Javanfekr Noda, Atsushi Liao, Ching-Wei Wakimoto, Arata Inoue, Yuri Jeon, Hyojung Takahashi, Satoru Hamada, Michito Exp Anim Original Adeno-associated virus serotype 9 (AAV9) has become a popular tool for gene transfer because of its ability to cross the blood-brain barrier and efficiently transduce genetic material into a variety of cell types. The study utilized GRR (Green-to-Red Reporter) mouse embryos, in which the expression of iCre results in the disappearance of Green Fluorescent Protein (GFP) expression and the detection of Discosoma sp. Red Fluorescent Protein (DsRed) expression by intraplacental injection. Our results demonstrate that AAV9-CMV-iCre can transduce multiple organs in embryos at developmental stages E9.5–E11.5, including the liver, heart, brain, thymus, and intestine. These findings suggest that intraplacental injection of AAV9-CMV-iCre is a viable method for the widespread transduction of GRR mouse embryos. Japanese Association for Laboratory Animal Science 2023-05-12 2023 /pmc/articles/PMC10658086/ /pubmed/37183025 http://dx.doi.org/10.1538/expanim.23-0044 Text en ©2023 Japanese Association for Laboratory Animal Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Original
Gogoleva, Natalia
Shahri, Zeynab Javanfekr
Noda, Atsushi
Liao, Ching-Wei
Wakimoto, Arata
Inoue, Yuri
Jeon, Hyojung
Takahashi, Satoru
Hamada, Michito
Intraplacental injection of AAV9-CMV-iCre results in the widespread transduction of multiple organs in double-reporter mouse embryos
title Intraplacental injection of AAV9-CMV-iCre results in the widespread transduction of multiple organs in double-reporter mouse embryos
title_full Intraplacental injection of AAV9-CMV-iCre results in the widespread transduction of multiple organs in double-reporter mouse embryos
title_fullStr Intraplacental injection of AAV9-CMV-iCre results in the widespread transduction of multiple organs in double-reporter mouse embryos
title_full_unstemmed Intraplacental injection of AAV9-CMV-iCre results in the widespread transduction of multiple organs in double-reporter mouse embryos
title_short Intraplacental injection of AAV9-CMV-iCre results in the widespread transduction of multiple organs in double-reporter mouse embryos
title_sort intraplacental injection of aav9-cmv-icre results in the widespread transduction of multiple organs in double-reporter mouse embryos
topic Original
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658086/
https://www.ncbi.nlm.nih.gov/pubmed/37183025
http://dx.doi.org/10.1538/expanim.23-0044
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