Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI

Combining histology and ex vivo MRI from the same mouse brain is a powerful way to study brain microstructure. Mouse brains prepared for ex vivo MRI are often kept in storage solution for months, potentially becoming brittle and showing reduced antigenicity. Here, we describe a protocol for mouse br...

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Detalles Bibliográficos
Autores principales: Smart, Adele, Tisca, Cristiana, Huszar, Istvan N., Kor, Daniel, Ansorge, Olaf, Tachrount, Mohamed, Smart, Sean, Lerch, Jason P., Miller, Karla L., Martins-Bach, Aurea B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658376/
https://www.ncbi.nlm.nih.gov/pubmed/37948184
http://dx.doi.org/10.1016/j.xpro.2023.102681
Descripción
Sumario:Combining histology and ex vivo MRI from the same mouse brain is a powerful way to study brain microstructure. Mouse brains prepared for ex vivo MRI are often kept in storage solution for months, potentially becoming brittle and showing reduced antigenicity. Here, we describe a protocol for mouse brain dissection, tissue processing, paraffin embedding, sectioning, and staining. We then detail registration of histology to ex vivo MRI data from the same sample and extraction of quantitative histological measurements.

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