Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI

Combining histology and ex vivo MRI from the same mouse brain is a powerful way to study brain microstructure. Mouse brains prepared for ex vivo MRI are often kept in storage solution for months, potentially becoming brittle and showing reduced antigenicity. Here, we describe a protocol for mouse br...

Descripción completa

Detalles Bibliográficos
Autores principales: Smart, Adele, Tisca, Cristiana, Huszar, Istvan N., Kor, Daniel, Ansorge, Olaf, Tachrount, Mohamed, Smart, Sean, Lerch, Jason P., Miller, Karla L., Martins-Bach, Aurea B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658376/
https://www.ncbi.nlm.nih.gov/pubmed/37948184
http://dx.doi.org/10.1016/j.xpro.2023.102681
_version_ 1785137403419164672
author Smart, Adele
Tisca, Cristiana
Huszar, Istvan N.
Kor, Daniel
Ansorge, Olaf
Tachrount, Mohamed
Smart, Sean
Lerch, Jason P.
Miller, Karla L.
Martins-Bach, Aurea B.
author_facet Smart, Adele
Tisca, Cristiana
Huszar, Istvan N.
Kor, Daniel
Ansorge, Olaf
Tachrount, Mohamed
Smart, Sean
Lerch, Jason P.
Miller, Karla L.
Martins-Bach, Aurea B.
author_sort Smart, Adele
collection PubMed
description Combining histology and ex vivo MRI from the same mouse brain is a powerful way to study brain microstructure. Mouse brains prepared for ex vivo MRI are often kept in storage solution for months, potentially becoming brittle and showing reduced antigenicity. Here, we describe a protocol for mouse brain dissection, tissue processing, paraffin embedding, sectioning, and staining. We then detail registration of histology to ex vivo MRI data from the same sample and extraction of quantitative histological measurements.
format Online
Article
Text
id pubmed-10658376
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-106583762023-11-09 Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI Smart, Adele Tisca, Cristiana Huszar, Istvan N. Kor, Daniel Ansorge, Olaf Tachrount, Mohamed Smart, Sean Lerch, Jason P. Miller, Karla L. Martins-Bach, Aurea B. STAR Protoc Protocol Combining histology and ex vivo MRI from the same mouse brain is a powerful way to study brain microstructure. Mouse brains prepared for ex vivo MRI are often kept in storage solution for months, potentially becoming brittle and showing reduced antigenicity. Here, we describe a protocol for mouse brain dissection, tissue processing, paraffin embedding, sectioning, and staining. We then detail registration of histology to ex vivo MRI data from the same sample and extraction of quantitative histological measurements. Elsevier 2023-11-09 /pmc/articles/PMC10658376/ /pubmed/37948184 http://dx.doi.org/10.1016/j.xpro.2023.102681 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Smart, Adele
Tisca, Cristiana
Huszar, Istvan N.
Kor, Daniel
Ansorge, Olaf
Tachrount, Mohamed
Smart, Sean
Lerch, Jason P.
Miller, Karla L.
Martins-Bach, Aurea B.
Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI
title Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI
title_full Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI
title_fullStr Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI
title_full_unstemmed Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI
title_short Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI
title_sort protocol for tissue processing and paraffin embedding of mouse brains following ex vivo mri
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658376/
https://www.ncbi.nlm.nih.gov/pubmed/37948184
http://dx.doi.org/10.1016/j.xpro.2023.102681
work_keys_str_mv AT smartadele protocolfortissueprocessingandparaffinembeddingofmousebrainsfollowingexvivomri
AT tiscacristiana protocolfortissueprocessingandparaffinembeddingofmousebrainsfollowingexvivomri
AT huszaristvann protocolfortissueprocessingandparaffinembeddingofmousebrainsfollowingexvivomri
AT kordaniel protocolfortissueprocessingandparaffinembeddingofmousebrainsfollowingexvivomri
AT ansorgeolaf protocolfortissueprocessingandparaffinembeddingofmousebrainsfollowingexvivomri
AT tachrountmohamed protocolfortissueprocessingandparaffinembeddingofmousebrainsfollowingexvivomri
AT smartsean protocolfortissueprocessingandparaffinembeddingofmousebrainsfollowingexvivomri
AT lerchjasonp protocolfortissueprocessingandparaffinembeddingofmousebrainsfollowingexvivomri
AT millerkarlal protocolfortissueprocessingandparaffinembeddingofmousebrainsfollowingexvivomri
AT martinsbachaureab protocolfortissueprocessingandparaffinembeddingofmousebrainsfollowingexvivomri

Ejemplares similares