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Photocatalytic effect of N–TiO(2) conjugated with folic acid against biofilm-forming resistant bacteria
Antibiotic resistance challenges the treatment of bacterial biofilm-related infections, but the use of nanoparticles as a treatment is a promising strategy to overcome bacterial infections. This study applied nitrogen-doped titanium dioxide (N–TiO(2)) conjugated with folic acid (FA) on biofilm-formi...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658382/ https://www.ncbi.nlm.nih.gov/pubmed/38027799 http://dx.doi.org/10.1016/j.heliyon.2023.e22108 |
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author | Oliveira, Raphaella I.S. de Oliveira, Iracema N. de Conto, Juliana F. de Souza, Augusto M. Batistuzzo de Medeiros, Silvia R. Egues, Silvia M. Padilha, Francine F. Hernández-Macedo, Maria L. |
author_facet | Oliveira, Raphaella I.S. de Oliveira, Iracema N. de Conto, Juliana F. de Souza, Augusto M. Batistuzzo de Medeiros, Silvia R. Egues, Silvia M. Padilha, Francine F. Hernández-Macedo, Maria L. |
author_sort | Oliveira, Raphaella I.S. |
collection | PubMed |
description | Antibiotic resistance challenges the treatment of bacterial biofilm-related infections, but the use of nanoparticles as a treatment is a promising strategy to overcome bacterial infections. This study applied nitrogen-doped titanium dioxide (N–TiO(2)) conjugated with folic acid (FA) on biofilm-forming resistant bacteria. The photocatalytic effect of TiO(2) nanoparticles (NPs) was studied under ultraviolet (UV), visible light, and dark conditions at 60, 120, and 180 min against planktonic cells and biofilms of Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), and Pseudomonas aeruginosa. TiO(2) NPs were in the anatase phase, spherical shaped with sizes of 10-13 nm, and effectively doped and conjugated with N and FA. The FA-conjugated nanoparticles (N–TiO(2)-FA and FA-TiO(2)) were shown to have a bactericidal effect on all bacteria between 60 and 180 min under UV and visible light conditions. Concerning biofilms, N–TiO(2)-FA was shown to have a highly disruptive effect on all bacterial biofilms under UV irradiation at 180 min. Meanwhile, the nanoparticles did not show DNA damaging potential and they had no cytostatic effect, indicating that these NPs are biocompatible. In sum, nanoparticle conjugation with FA promoted photocatalytic effectiveness, revealing the promise this nanomaterial holds as a biocompatible antimicrobial agent. |
format | Online Article Text |
id | pubmed-10658382 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-106583822023-11-07 Photocatalytic effect of N–TiO(2) conjugated with folic acid against biofilm-forming resistant bacteria Oliveira, Raphaella I.S. de Oliveira, Iracema N. de Conto, Juliana F. de Souza, Augusto M. Batistuzzo de Medeiros, Silvia R. Egues, Silvia M. Padilha, Francine F. Hernández-Macedo, Maria L. Heliyon Research Article Antibiotic resistance challenges the treatment of bacterial biofilm-related infections, but the use of nanoparticles as a treatment is a promising strategy to overcome bacterial infections. This study applied nitrogen-doped titanium dioxide (N–TiO(2)) conjugated with folic acid (FA) on biofilm-forming resistant bacteria. The photocatalytic effect of TiO(2) nanoparticles (NPs) was studied under ultraviolet (UV), visible light, and dark conditions at 60, 120, and 180 min against planktonic cells and biofilms of Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), and Pseudomonas aeruginosa. TiO(2) NPs were in the anatase phase, spherical shaped with sizes of 10-13 nm, and effectively doped and conjugated with N and FA. The FA-conjugated nanoparticles (N–TiO(2)-FA and FA-TiO(2)) were shown to have a bactericidal effect on all bacteria between 60 and 180 min under UV and visible light conditions. Concerning biofilms, N–TiO(2)-FA was shown to have a highly disruptive effect on all bacterial biofilms under UV irradiation at 180 min. Meanwhile, the nanoparticles did not show DNA damaging potential and they had no cytostatic effect, indicating that these NPs are biocompatible. In sum, nanoparticle conjugation with FA promoted photocatalytic effectiveness, revealing the promise this nanomaterial holds as a biocompatible antimicrobial agent. Elsevier 2023-11-07 /pmc/articles/PMC10658382/ /pubmed/38027799 http://dx.doi.org/10.1016/j.heliyon.2023.e22108 Text en © 2023 The Authors. Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Oliveira, Raphaella I.S. de Oliveira, Iracema N. de Conto, Juliana F. de Souza, Augusto M. Batistuzzo de Medeiros, Silvia R. Egues, Silvia M. Padilha, Francine F. Hernández-Macedo, Maria L. Photocatalytic effect of N–TiO(2) conjugated with folic acid against biofilm-forming resistant bacteria |
title | Photocatalytic effect of N–TiO(2) conjugated with folic acid against biofilm-forming resistant bacteria |
title_full | Photocatalytic effect of N–TiO(2) conjugated with folic acid against biofilm-forming resistant bacteria |
title_fullStr | Photocatalytic effect of N–TiO(2) conjugated with folic acid against biofilm-forming resistant bacteria |
title_full_unstemmed | Photocatalytic effect of N–TiO(2) conjugated with folic acid against biofilm-forming resistant bacteria |
title_short | Photocatalytic effect of N–TiO(2) conjugated with folic acid against biofilm-forming resistant bacteria |
title_sort | photocatalytic effect of n–tio(2) conjugated with folic acid against biofilm-forming resistant bacteria |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658382/ https://www.ncbi.nlm.nih.gov/pubmed/38027799 http://dx.doi.org/10.1016/j.heliyon.2023.e22108 |
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