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Photocatalytic effect of N–TiO(2) conjugated with folic acid against biofilm-forming resistant bacteria

Antibiotic resistance challenges the treatment of bacterial biofilm-related infections, but the use of nanoparticles as a treatment is a promising strategy to overcome bacterial infections. This study applied nitrogen-doped titanium dioxide (N–TiO(2)) conjugated with folic acid (FA) on biofilm-formi...

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Autores principales: Oliveira, Raphaella I.S., de Oliveira, Iracema N., de Conto, Juliana F., de Souza, Augusto M., Batistuzzo de Medeiros, Silvia R., Egues, Silvia M., Padilha, Francine F., Hernández-Macedo, Maria L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658382/
https://www.ncbi.nlm.nih.gov/pubmed/38027799
http://dx.doi.org/10.1016/j.heliyon.2023.e22108
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author Oliveira, Raphaella I.S.
de Oliveira, Iracema N.
de Conto, Juliana F.
de Souza, Augusto M.
Batistuzzo de Medeiros, Silvia R.
Egues, Silvia M.
Padilha, Francine F.
Hernández-Macedo, Maria L.
author_facet Oliveira, Raphaella I.S.
de Oliveira, Iracema N.
de Conto, Juliana F.
de Souza, Augusto M.
Batistuzzo de Medeiros, Silvia R.
Egues, Silvia M.
Padilha, Francine F.
Hernández-Macedo, Maria L.
author_sort Oliveira, Raphaella I.S.
collection PubMed
description Antibiotic resistance challenges the treatment of bacterial biofilm-related infections, but the use of nanoparticles as a treatment is a promising strategy to overcome bacterial infections. This study applied nitrogen-doped titanium dioxide (N–TiO(2)) conjugated with folic acid (FA) on biofilm-forming resistant bacteria. The photocatalytic effect of TiO(2) nanoparticles (NPs) was studied under ultraviolet (UV), visible light, and dark conditions at 60, 120, and 180 min against planktonic cells and biofilms of Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), and Pseudomonas aeruginosa. TiO(2) NPs were in the anatase phase, spherical shaped with sizes of 10-13 nm, and effectively doped and conjugated with N and FA. The FA-conjugated nanoparticles (N–TiO(2)-FA and FA-TiO(2)) were shown to have a bactericidal effect on all bacteria between 60 and 180 min under UV and visible light conditions. Concerning biofilms, N–TiO(2)-FA was shown to have a highly disruptive effect on all bacterial biofilms under UV irradiation at 180 min. Meanwhile, the nanoparticles did not show DNA damaging potential and they had no cytostatic effect, indicating that these NPs are biocompatible. In sum, nanoparticle conjugation with FA promoted photocatalytic effectiveness, revealing the promise this nanomaterial holds as a biocompatible antimicrobial agent.
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spelling pubmed-106583822023-11-07 Photocatalytic effect of N–TiO(2) conjugated with folic acid against biofilm-forming resistant bacteria Oliveira, Raphaella I.S. de Oliveira, Iracema N. de Conto, Juliana F. de Souza, Augusto M. Batistuzzo de Medeiros, Silvia R. Egues, Silvia M. Padilha, Francine F. Hernández-Macedo, Maria L. Heliyon Research Article Antibiotic resistance challenges the treatment of bacterial biofilm-related infections, but the use of nanoparticles as a treatment is a promising strategy to overcome bacterial infections. This study applied nitrogen-doped titanium dioxide (N–TiO(2)) conjugated with folic acid (FA) on biofilm-forming resistant bacteria. The photocatalytic effect of TiO(2) nanoparticles (NPs) was studied under ultraviolet (UV), visible light, and dark conditions at 60, 120, and 180 min against planktonic cells and biofilms of Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), and Pseudomonas aeruginosa. TiO(2) NPs were in the anatase phase, spherical shaped with sizes of 10-13 nm, and effectively doped and conjugated with N and FA. The FA-conjugated nanoparticles (N–TiO(2)-FA and FA-TiO(2)) were shown to have a bactericidal effect on all bacteria between 60 and 180 min under UV and visible light conditions. Concerning biofilms, N–TiO(2)-FA was shown to have a highly disruptive effect on all bacterial biofilms under UV irradiation at 180 min. Meanwhile, the nanoparticles did not show DNA damaging potential and they had no cytostatic effect, indicating that these NPs are biocompatible. In sum, nanoparticle conjugation with FA promoted photocatalytic effectiveness, revealing the promise this nanomaterial holds as a biocompatible antimicrobial agent. Elsevier 2023-11-07 /pmc/articles/PMC10658382/ /pubmed/38027799 http://dx.doi.org/10.1016/j.heliyon.2023.e22108 Text en © 2023 The Authors. Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Oliveira, Raphaella I.S.
de Oliveira, Iracema N.
de Conto, Juliana F.
de Souza, Augusto M.
Batistuzzo de Medeiros, Silvia R.
Egues, Silvia M.
Padilha, Francine F.
Hernández-Macedo, Maria L.
Photocatalytic effect of N–TiO(2) conjugated with folic acid against biofilm-forming resistant bacteria
title Photocatalytic effect of N–TiO(2) conjugated with folic acid against biofilm-forming resistant bacteria
title_full Photocatalytic effect of N–TiO(2) conjugated with folic acid against biofilm-forming resistant bacteria
title_fullStr Photocatalytic effect of N–TiO(2) conjugated with folic acid against biofilm-forming resistant bacteria
title_full_unstemmed Photocatalytic effect of N–TiO(2) conjugated with folic acid against biofilm-forming resistant bacteria
title_short Photocatalytic effect of N–TiO(2) conjugated with folic acid against biofilm-forming resistant bacteria
title_sort photocatalytic effect of n–tio(2) conjugated with folic acid against biofilm-forming resistant bacteria
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658382/
https://www.ncbi.nlm.nih.gov/pubmed/38027799
http://dx.doi.org/10.1016/j.heliyon.2023.e22108
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