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Novel metal peroxide nanoboxes restrain Clostridioides difficile infection beyond the bactericidal and sporicidal activity

Clostridioides difficile spores are considered as the major source responsible for the development of C. difficile infection (CDI), which is associated with an increased risk of death in patients and has become an important issue in infection control of nosocomial infections. Current treatment again...

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Autores principales: Yang, Li‐Xing, Lai, Yi‐Hsin, Cheung, Chun In, Ye, Zhi, Huang, Tzu‐Chi, Wang, Yu‐Chin, Chin, Yu‐Cheng, Chia, Zi‐Chun, Chen, Ya‐Jyun, Li, Meng‐Jia, Tseng, Hsiu‐Ying, Tsai, Yi‐Tseng, Zhang, Zhi‐Bin, Chen, Kuan‐Hsu, Tsai, Bo‐Yang, Shieh, Dar‐Bin, Lee, Nan‐Yao, Tsai, Pei‐Jane, Huang, Chih‐Chia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658501/
https://www.ncbi.nlm.nih.gov/pubmed/38023694
http://dx.doi.org/10.1002/btm2.10593
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author Yang, Li‐Xing
Lai, Yi‐Hsin
Cheung, Chun In
Ye, Zhi
Huang, Tzu‐Chi
Wang, Yu‐Chin
Chin, Yu‐Cheng
Chia, Zi‐Chun
Chen, Ya‐Jyun
Li, Meng‐Jia
Tseng, Hsiu‐Ying
Tsai, Yi‐Tseng
Zhang, Zhi‐Bin
Chen, Kuan‐Hsu
Tsai, Bo‐Yang
Shieh, Dar‐Bin
Lee, Nan‐Yao
Tsai, Pei‐Jane
Huang, Chih‐Chia
author_facet Yang, Li‐Xing
Lai, Yi‐Hsin
Cheung, Chun In
Ye, Zhi
Huang, Tzu‐Chi
Wang, Yu‐Chin
Chin, Yu‐Cheng
Chia, Zi‐Chun
Chen, Ya‐Jyun
Li, Meng‐Jia
Tseng, Hsiu‐Ying
Tsai, Yi‐Tseng
Zhang, Zhi‐Bin
Chen, Kuan‐Hsu
Tsai, Bo‐Yang
Shieh, Dar‐Bin
Lee, Nan‐Yao
Tsai, Pei‐Jane
Huang, Chih‐Chia
author_sort Yang, Li‐Xing
collection PubMed
description Clostridioides difficile spores are considered as the major source responsible for the development of C. difficile infection (CDI), which is associated with an increased risk of death in patients and has become an important issue in infection control of nosocomial infections. Current treatment against CDI still relies on antibiotics, which also damage normal flora and increase the risk of CDI recurrence. Therefore, alternative therapies that are more effective against C. difficile bacteria and spores are urgently needed. Here, we designed an oxidation process using H(2)O(2) containing PBS solution to generate Cl(−) and peroxide molecules that further process Ag and Au ions to form nanoboxes with Ag–Au peroxide coat covering Au shell and AgCl core (AgAu‐based nanoboxes). The AgAu‐based nanoboxes efficiently disrupted the membrane structure of bacteria/spores of C. difficile after 30–45 min exposure to the highly reactive Ag/Au peroxide surface of the nano structures. The Au‐enclosed AgCl provided sustained suppression of the growth of 2 × 10(7) pathogenic Escherichia coli for up to 19 days. In a fecal bench ex vivo test and in vivo CDI murine model, biocompatibility and therapeutic efficacy of the AuAg nanoboxes to attenuate CDI was demonstrated by restoring the gut microbiota and colon mucosal structure. The treatment successfully rescued the CDI mice from death and prevented their recurrence mediated by vancomycin treatment. The significant outcomes indicated that the new peroxide‐derived AgAu‐based nanoboxes possess great potential for future translation into clinical application as a new alternative therapeutic strategy against CDI.
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spelling pubmed-106585012023-09-05 Novel metal peroxide nanoboxes restrain Clostridioides difficile infection beyond the bactericidal and sporicidal activity Yang, Li‐Xing Lai, Yi‐Hsin Cheung, Chun In Ye, Zhi Huang, Tzu‐Chi Wang, Yu‐Chin Chin, Yu‐Cheng Chia, Zi‐Chun Chen, Ya‐Jyun Li, Meng‐Jia Tseng, Hsiu‐Ying Tsai, Yi‐Tseng Zhang, Zhi‐Bin Chen, Kuan‐Hsu Tsai, Bo‐Yang Shieh, Dar‐Bin Lee, Nan‐Yao Tsai, Pei‐Jane Huang, Chih‐Chia Bioeng Transl Med Special Issue Articles Clostridioides difficile spores are considered as the major source responsible for the development of C. difficile infection (CDI), which is associated with an increased risk of death in patients and has become an important issue in infection control of nosocomial infections. Current treatment against CDI still relies on antibiotics, which also damage normal flora and increase the risk of CDI recurrence. Therefore, alternative therapies that are more effective against C. difficile bacteria and spores are urgently needed. Here, we designed an oxidation process using H(2)O(2) containing PBS solution to generate Cl(−) and peroxide molecules that further process Ag and Au ions to form nanoboxes with Ag–Au peroxide coat covering Au shell and AgCl core (AgAu‐based nanoboxes). The AgAu‐based nanoboxes efficiently disrupted the membrane structure of bacteria/spores of C. difficile after 30–45 min exposure to the highly reactive Ag/Au peroxide surface of the nano structures. The Au‐enclosed AgCl provided sustained suppression of the growth of 2 × 10(7) pathogenic Escherichia coli for up to 19 days. In a fecal bench ex vivo test and in vivo CDI murine model, biocompatibility and therapeutic efficacy of the AuAg nanoboxes to attenuate CDI was demonstrated by restoring the gut microbiota and colon mucosal structure. The treatment successfully rescued the CDI mice from death and prevented their recurrence mediated by vancomycin treatment. The significant outcomes indicated that the new peroxide‐derived AgAu‐based nanoboxes possess great potential for future translation into clinical application as a new alternative therapeutic strategy against CDI. John Wiley & Sons, Inc. 2023-09-05 /pmc/articles/PMC10658501/ /pubmed/38023694 http://dx.doi.org/10.1002/btm2.10593 Text en © 2023 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of The American Institute of Chemical Engineers. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Special Issue Articles
Yang, Li‐Xing
Lai, Yi‐Hsin
Cheung, Chun In
Ye, Zhi
Huang, Tzu‐Chi
Wang, Yu‐Chin
Chin, Yu‐Cheng
Chia, Zi‐Chun
Chen, Ya‐Jyun
Li, Meng‐Jia
Tseng, Hsiu‐Ying
Tsai, Yi‐Tseng
Zhang, Zhi‐Bin
Chen, Kuan‐Hsu
Tsai, Bo‐Yang
Shieh, Dar‐Bin
Lee, Nan‐Yao
Tsai, Pei‐Jane
Huang, Chih‐Chia
Novel metal peroxide nanoboxes restrain Clostridioides difficile infection beyond the bactericidal and sporicidal activity
title Novel metal peroxide nanoboxes restrain Clostridioides difficile infection beyond the bactericidal and sporicidal activity
title_full Novel metal peroxide nanoboxes restrain Clostridioides difficile infection beyond the bactericidal and sporicidal activity
title_fullStr Novel metal peroxide nanoboxes restrain Clostridioides difficile infection beyond the bactericidal and sporicidal activity
title_full_unstemmed Novel metal peroxide nanoboxes restrain Clostridioides difficile infection beyond the bactericidal and sporicidal activity
title_short Novel metal peroxide nanoboxes restrain Clostridioides difficile infection beyond the bactericidal and sporicidal activity
title_sort novel metal peroxide nanoboxes restrain clostridioides difficile infection beyond the bactericidal and sporicidal activity
topic Special Issue Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658501/
https://www.ncbi.nlm.nih.gov/pubmed/38023694
http://dx.doi.org/10.1002/btm2.10593
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