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Injection of a PMMA‐doped MSC spheroid gel for the treatment of painful osteoporotic vertebral compression fractures

We aimed to develop a biocompatible treatment to overcome the limitations of polymethyl methacrylate (PMMA) vertebroplasty for osteoporotic compression fracture patients. We synthesized an injectable hydrogel containing PMMA. Mesenchymal stem cell (MSC) spheroids were included in the injectable PMMA...

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Detalles Bibliográficos
Autores principales: Ko, Wan‐Kyu, Lee, Daye, Kim, Seong Jun, Han, Gong Ho, Lee, Donghyun, Sheen, Seung Hun, Sohn, Seil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658584/
https://www.ncbi.nlm.nih.gov/pubmed/38023703
http://dx.doi.org/10.1002/btm2.10577
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author Ko, Wan‐Kyu
Lee, Daye
Kim, Seong Jun
Han, Gong Ho
Lee, Donghyun
Sheen, Seung Hun
Sohn, Seil
author_facet Ko, Wan‐Kyu
Lee, Daye
Kim, Seong Jun
Han, Gong Ho
Lee, Donghyun
Sheen, Seung Hun
Sohn, Seil
author_sort Ko, Wan‐Kyu
collection PubMed
description We aimed to develop a biocompatible treatment to overcome the limitations of polymethyl methacrylate (PMMA) vertebroplasty for osteoporotic compression fracture patients. We synthesized an injectable hydrogel containing PMMA. Mesenchymal stem cell (MSC) spheroids were included in the injectable PMMA‐doped gel (= PMMA‐doped spheroid gel). In vitro, the osteogenic/anti‐inflammatory effects of the embedded spheroids were investigated by the quantitative real‐time polymerase chain reaction method. In vivo, we used ovariectomy (OVX)‐induced osteoporotic rats with injured femurs to investigate the pain‐relief effects. The OVX rats were divided into four groups according to the materials injected (non, PMMA, PMMA gel, and PMMA‐spheroid gel) into the lesion. The immunofluorescence (IF) intensity levels of painful markers in dorsal root ganglia (DRG) were measured. In vitro, a volumetric ratio of the gel of 8 (gel):2 (PMMA) was non‐cytotoxic for MSCs and promoted the expression of osteogenic/anti‐inflammatory markers. In vivo, the values of several bone parameters in the PMMA‐doped spheroid gel group showed remarkable increases compared to those in the PMMA group. In addition, the IF intensity levels of the painful markers were noticeably decreased in the PMMA‐spheroid gel group. We, therefore, suggest that this treatment can be useful for osteoporotic vertebral compression fracture patients.
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spelling pubmed-106585842023-07-16 Injection of a PMMA‐doped MSC spheroid gel for the treatment of painful osteoporotic vertebral compression fractures Ko, Wan‐Kyu Lee, Daye Kim, Seong Jun Han, Gong Ho Lee, Donghyun Sheen, Seung Hun Sohn, Seil Bioeng Transl Med Regular Issue Articles We aimed to develop a biocompatible treatment to overcome the limitations of polymethyl methacrylate (PMMA) vertebroplasty for osteoporotic compression fracture patients. We synthesized an injectable hydrogel containing PMMA. Mesenchymal stem cell (MSC) spheroids were included in the injectable PMMA‐doped gel (= PMMA‐doped spheroid gel). In vitro, the osteogenic/anti‐inflammatory effects of the embedded spheroids were investigated by the quantitative real‐time polymerase chain reaction method. In vivo, we used ovariectomy (OVX)‐induced osteoporotic rats with injured femurs to investigate the pain‐relief effects. The OVX rats were divided into four groups according to the materials injected (non, PMMA, PMMA gel, and PMMA‐spheroid gel) into the lesion. The immunofluorescence (IF) intensity levels of painful markers in dorsal root ganglia (DRG) were measured. In vitro, a volumetric ratio of the gel of 8 (gel):2 (PMMA) was non‐cytotoxic for MSCs and promoted the expression of osteogenic/anti‐inflammatory markers. In vivo, the values of several bone parameters in the PMMA‐doped spheroid gel group showed remarkable increases compared to those in the PMMA group. In addition, the IF intensity levels of the painful markers were noticeably decreased in the PMMA‐spheroid gel group. We, therefore, suggest that this treatment can be useful for osteoporotic vertebral compression fracture patients. John Wiley & Sons, Inc. 2023-07-16 /pmc/articles/PMC10658584/ /pubmed/38023703 http://dx.doi.org/10.1002/btm2.10577 Text en © 2023 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Regular Issue Articles
Ko, Wan‐Kyu
Lee, Daye
Kim, Seong Jun
Han, Gong Ho
Lee, Donghyun
Sheen, Seung Hun
Sohn, Seil
Injection of a PMMA‐doped MSC spheroid gel for the treatment of painful osteoporotic vertebral compression fractures
title Injection of a PMMA‐doped MSC spheroid gel for the treatment of painful osteoporotic vertebral compression fractures
title_full Injection of a PMMA‐doped MSC spheroid gel for the treatment of painful osteoporotic vertebral compression fractures
title_fullStr Injection of a PMMA‐doped MSC spheroid gel for the treatment of painful osteoporotic vertebral compression fractures
title_full_unstemmed Injection of a PMMA‐doped MSC spheroid gel for the treatment of painful osteoporotic vertebral compression fractures
title_short Injection of a PMMA‐doped MSC spheroid gel for the treatment of painful osteoporotic vertebral compression fractures
title_sort injection of a pmma‐doped msc spheroid gel for the treatment of painful osteoporotic vertebral compression fractures
topic Regular Issue Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658584/
https://www.ncbi.nlm.nih.gov/pubmed/38023703
http://dx.doi.org/10.1002/btm2.10577
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