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Metabolomics analysis of pathways underlying radiation-induced salivary gland dysfunction stages
Salivary gland hypofunction is an adverse side effect associated with radiotherapy for head and neck cancer patients. This study delineated metabolic changes at acute, intermediate, and chronic radiation damage response stages in mouse salivary glands following a single 5 Gy dose. Ultra-high perform...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10659204/ https://www.ncbi.nlm.nih.gov/pubmed/37983277 http://dx.doi.org/10.1371/journal.pone.0294355 |
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author | Buss, Lauren G. De Oliveira Pessoa, Diogo Snider, Justin M. Padi, Megha Martinez, Jessica A. Limesand, Kirsten H. |
author_facet | Buss, Lauren G. De Oliveira Pessoa, Diogo Snider, Justin M. Padi, Megha Martinez, Jessica A. Limesand, Kirsten H. |
author_sort | Buss, Lauren G. |
collection | PubMed |
description | Salivary gland hypofunction is an adverse side effect associated with radiotherapy for head and neck cancer patients. This study delineated metabolic changes at acute, intermediate, and chronic radiation damage response stages in mouse salivary glands following a single 5 Gy dose. Ultra-high performance liquid chromatography-mass spectrometry was performed on parotid salivary gland tissue collected at 3, 14, and 30 days following radiation (IR). Pathway enrichment analysis, network analysis based on metabolite structural similarity, and network analysis based on metabolite abundance correlations were used to incorporate both metabolite levels and structural annotation. The greatest number of enriched pathways are observed at 3 days and the lowest at 30 days following radiation. Amino acid metabolism pathways, glutathione metabolism, and central carbon metabolism in cancer are enriched at all radiation time points across different analytical methods. This study suggests that glutathione and central carbon metabolism in cancer may be important pathways in the unresolved effect of radiation treatment. |
format | Online Article Text |
id | pubmed-10659204 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-106592042023-11-20 Metabolomics analysis of pathways underlying radiation-induced salivary gland dysfunction stages Buss, Lauren G. De Oliveira Pessoa, Diogo Snider, Justin M. Padi, Megha Martinez, Jessica A. Limesand, Kirsten H. PLoS One Research Article Salivary gland hypofunction is an adverse side effect associated with radiotherapy for head and neck cancer patients. This study delineated metabolic changes at acute, intermediate, and chronic radiation damage response stages in mouse salivary glands following a single 5 Gy dose. Ultra-high performance liquid chromatography-mass spectrometry was performed on parotid salivary gland tissue collected at 3, 14, and 30 days following radiation (IR). Pathway enrichment analysis, network analysis based on metabolite structural similarity, and network analysis based on metabolite abundance correlations were used to incorporate both metabolite levels and structural annotation. The greatest number of enriched pathways are observed at 3 days and the lowest at 30 days following radiation. Amino acid metabolism pathways, glutathione metabolism, and central carbon metabolism in cancer are enriched at all radiation time points across different analytical methods. This study suggests that glutathione and central carbon metabolism in cancer may be important pathways in the unresolved effect of radiation treatment. Public Library of Science 2023-11-20 /pmc/articles/PMC10659204/ /pubmed/37983277 http://dx.doi.org/10.1371/journal.pone.0294355 Text en © 2023 Buss et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Buss, Lauren G. De Oliveira Pessoa, Diogo Snider, Justin M. Padi, Megha Martinez, Jessica A. Limesand, Kirsten H. Metabolomics analysis of pathways underlying radiation-induced salivary gland dysfunction stages |
title | Metabolomics analysis of pathways underlying radiation-induced salivary gland dysfunction stages |
title_full | Metabolomics analysis of pathways underlying radiation-induced salivary gland dysfunction stages |
title_fullStr | Metabolomics analysis of pathways underlying radiation-induced salivary gland dysfunction stages |
title_full_unstemmed | Metabolomics analysis of pathways underlying radiation-induced salivary gland dysfunction stages |
title_short | Metabolomics analysis of pathways underlying radiation-induced salivary gland dysfunction stages |
title_sort | metabolomics analysis of pathways underlying radiation-induced salivary gland dysfunction stages |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10659204/ https://www.ncbi.nlm.nih.gov/pubmed/37983277 http://dx.doi.org/10.1371/journal.pone.0294355 |
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