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Increased Glucagon Immunoreactivity in a Rat Model of Diet-Induced Obesity following Sleeve Gastrectomy

OBJECTIVE: Bariatric surgery is currently the most effective treatment for obesity, and procedures such as Roux-en-Y gastric bypass and sleeve gastrectomy (SG) also result in rapid improvements in insulin sensitivity and glucose tolerance. In addition, these procedures cause changes in the secretion...

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Autores principales: Al-Sabah, Suleiman, Jamal, Mohammad H., Al-Khaledi, Ghanim, Dsouza, Carol, AlOtaibi, Fatemah, Al-Ali, Waleed, Cherian, Preethi, Al-Khairi, Irina, Ali, Hamad, Abu-Farha, Mohamed, Abubaker, Jehad, Al-Mulla, Fahd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: S. Karger AG 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10659591/
https://www.ncbi.nlm.nih.gov/pubmed/37634505
http://dx.doi.org/10.1159/000533746
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author Al-Sabah, Suleiman
Jamal, Mohammad H.
Al-Khaledi, Ghanim
Dsouza, Carol
AlOtaibi, Fatemah
Al-Ali, Waleed
Cherian, Preethi
Al-Khairi, Irina
Ali, Hamad
Abu-Farha, Mohamed
Abubaker, Jehad
Al-Mulla, Fahd
author_facet Al-Sabah, Suleiman
Jamal, Mohammad H.
Al-Khaledi, Ghanim
Dsouza, Carol
AlOtaibi, Fatemah
Al-Ali, Waleed
Cherian, Preethi
Al-Khairi, Irina
Ali, Hamad
Abu-Farha, Mohamed
Abubaker, Jehad
Al-Mulla, Fahd
author_sort Al-Sabah, Suleiman
collection PubMed
description OBJECTIVE: Bariatric surgery is currently the most effective treatment for obesity, and procedures such as Roux-en-Y gastric bypass and sleeve gastrectomy (SG) also result in rapid improvements in insulin sensitivity and glucose tolerance. In addition, these procedures cause changes in the secretion of various gut-derived hormones. The role these hormones play in the mechanism of the beneficial effects of bariatric surgery is still debated, but nonetheless, their importance provides inspiration for novel obesity-targeted pharmacotherapies. METHODS: Male Sprague-Dawley rats were fed either regular chow or a cafeteria diet to induce obesity. A sub-group of the obese animals then underwent either sham surgery or SG. RESULTS: Following a 4-week recovery period, SG rats weighed significantly less than obese or sham-operated rats. Improvements in glucose tolerance and insulin sensitivity also occurred in the SG group, but these were not always statistically significant. We measured the intracellular lipid content of liver samples and found that obese rats showed signs of non-alcoholic fatty liver disease, which were significantly ameliorated by SG. There were significantly higher glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide responses to a standard mixed meal in the SG group, as well as paradoxically higher glucagon secretion. CONCLUSION: These data highlight the need for more specific anti-glucagon antibodies to characterize the changes in proglucagon-derived peptide concentrations that occur following SG. Further studies are required to determine whether these peptides contribute to the therapeutic effects of SG.
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spelling pubmed-106595912023-11-01 Increased Glucagon Immunoreactivity in a Rat Model of Diet-Induced Obesity following Sleeve Gastrectomy Al-Sabah, Suleiman Jamal, Mohammad H. Al-Khaledi, Ghanim Dsouza, Carol AlOtaibi, Fatemah Al-Ali, Waleed Cherian, Preethi Al-Khairi, Irina Ali, Hamad Abu-Farha, Mohamed Abubaker, Jehad Al-Mulla, Fahd Med Princ Pract Original Paper OBJECTIVE: Bariatric surgery is currently the most effective treatment for obesity, and procedures such as Roux-en-Y gastric bypass and sleeve gastrectomy (SG) also result in rapid improvements in insulin sensitivity and glucose tolerance. In addition, these procedures cause changes in the secretion of various gut-derived hormones. The role these hormones play in the mechanism of the beneficial effects of bariatric surgery is still debated, but nonetheless, their importance provides inspiration for novel obesity-targeted pharmacotherapies. METHODS: Male Sprague-Dawley rats were fed either regular chow or a cafeteria diet to induce obesity. A sub-group of the obese animals then underwent either sham surgery or SG. RESULTS: Following a 4-week recovery period, SG rats weighed significantly less than obese or sham-operated rats. Improvements in glucose tolerance and insulin sensitivity also occurred in the SG group, but these were not always statistically significant. We measured the intracellular lipid content of liver samples and found that obese rats showed signs of non-alcoholic fatty liver disease, which were significantly ameliorated by SG. There were significantly higher glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide responses to a standard mixed meal in the SG group, as well as paradoxically higher glucagon secretion. CONCLUSION: These data highlight the need for more specific anti-glucagon antibodies to characterize the changes in proglucagon-derived peptide concentrations that occur following SG. Further studies are required to determine whether these peptides contribute to the therapeutic effects of SG. S. Karger AG 2023-08-25 /pmc/articles/PMC10659591/ /pubmed/37634505 http://dx.doi.org/10.1159/000533746 Text en © 2023 The Author(s). Published by S. Karger AG, Basel https://creativecommons.org/licenses/by-nc/4.0/This article is licensed under the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC) (http://www.karger.com/Services/OpenAccessLicense). Usage and distribution for commercial purposes requires written permission.
spellingShingle Original Paper
Al-Sabah, Suleiman
Jamal, Mohammad H.
Al-Khaledi, Ghanim
Dsouza, Carol
AlOtaibi, Fatemah
Al-Ali, Waleed
Cherian, Preethi
Al-Khairi, Irina
Ali, Hamad
Abu-Farha, Mohamed
Abubaker, Jehad
Al-Mulla, Fahd
Increased Glucagon Immunoreactivity in a Rat Model of Diet-Induced Obesity following Sleeve Gastrectomy
title Increased Glucagon Immunoreactivity in a Rat Model of Diet-Induced Obesity following Sleeve Gastrectomy
title_full Increased Glucagon Immunoreactivity in a Rat Model of Diet-Induced Obesity following Sleeve Gastrectomy
title_fullStr Increased Glucagon Immunoreactivity in a Rat Model of Diet-Induced Obesity following Sleeve Gastrectomy
title_full_unstemmed Increased Glucagon Immunoreactivity in a Rat Model of Diet-Induced Obesity following Sleeve Gastrectomy
title_short Increased Glucagon Immunoreactivity in a Rat Model of Diet-Induced Obesity following Sleeve Gastrectomy
title_sort increased glucagon immunoreactivity in a rat model of diet-induced obesity following sleeve gastrectomy
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10659591/
https://www.ncbi.nlm.nih.gov/pubmed/37634505
http://dx.doi.org/10.1159/000533746
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