Comparing the effects of chemical Ca(2+) dyes and R-GECO on contractility and Ca(2+) transients in adult and human iPSC cardiomyocytes

We compared commonly used BAPTA-derived chemical Ca(2+) dyes (fura2, Fluo-4, and Rhod-2) with a newer genetically encoded indicator (R-GECO) in single cell models of the heart. We assessed their performance and effects on cardiomyocyte contractility, determining fluorescent signal-to-noise ratios an...

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Detalles Bibliográficos
Autores principales: Robinson, Paul, Sparrow, Alexander J., Psaras, Yiangos, Steeples, Violetta, Simon, Jillian N., Broyles, Connor N., Chang, Yu-Fen, Brook, Frances A., Wang, Ying-Jie, Blease, Andrew, Zhang, Xiaoyu, Abassi, Yama A., Geeves, Michael A., Toepfer, Christopher N., Watkins, Hugh, Redwood, Charles, Daniels, Matthew J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10659987/
https://www.ncbi.nlm.nih.gov/pubmed/37127261
http://dx.doi.org/10.1016/j.yjmcc.2023.04.008

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