Cargando…

Bio-SELEX: A Strategy for Biomarkers Isolation Directly from Biological Samples

Bio-SELEX is a revolutionary method for the discovery of novel biomarkers within biological samples, offering profound insights into diagnosing both infectious and non-infectious diseases. This innovative strategy involves three crucial steps: Traditional SELEX, Pull Down, and mass spectrometry. Fir...

Descripción completa

Detalles Bibliográficos
Autores principales: Ospina-Villa, Juan David, Restrepo-Cano, Valentina, Sánchez-Jiménez, Miryan Margot
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10660531/
https://www.ncbi.nlm.nih.gov/pubmed/37987356
http://dx.doi.org/10.3390/mps6060109
_version_ 1785137778374213632
author Ospina-Villa, Juan David
Restrepo-Cano, Valentina
Sánchez-Jiménez, Miryan Margot
author_facet Ospina-Villa, Juan David
Restrepo-Cano, Valentina
Sánchez-Jiménez, Miryan Margot
author_sort Ospina-Villa, Juan David
collection PubMed
description Bio-SELEX is a revolutionary method for the discovery of novel biomarkers within biological samples, offering profound insights into diagnosing both infectious and non-infectious diseases. This innovative strategy involves three crucial steps: Traditional SELEX, Pull Down, and mass spectrometry. Firstly, Traditional SELEX involves the systematic selection of specific nucleic acid sequences (aptamers) that bind to the target molecules of interest. These aptamers are generated through iterative rounds of selection, amplification, and enrichment, ultimately yielding highly selective ligands. Secondly, the Pull-Down phase employs these aptamers to capture and isolate the target biomarkers from complex biological samples. This step ensures the specificity of the selected aptamers in binding to their intended targets. Lastly, mass spectrometry is utilized to identify and quantify the captured biomarkers, providing precise information about their presence and concentration in the sample. These quantitative data are invaluable in disease diagnosis and monitoring. Bio-SELEX’s significance lies in its ability to discover biomarkers for a wide range of diseases, spanning infectious and non-infectious conditions. This approach holds great promise for early disease detection, personalized medicine, and the development of targeted therapies. By harnessing the power of aptamers and mass spectrometry, Bio-SELEX advances our understanding of disease biology and opens new avenues for improved healthcare.
format Online
Article
Text
id pubmed-10660531
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-106605312023-11-11 Bio-SELEX: A Strategy for Biomarkers Isolation Directly from Biological Samples Ospina-Villa, Juan David Restrepo-Cano, Valentina Sánchez-Jiménez, Miryan Margot Methods Protoc Protocol Bio-SELEX is a revolutionary method for the discovery of novel biomarkers within biological samples, offering profound insights into diagnosing both infectious and non-infectious diseases. This innovative strategy involves three crucial steps: Traditional SELEX, Pull Down, and mass spectrometry. Firstly, Traditional SELEX involves the systematic selection of specific nucleic acid sequences (aptamers) that bind to the target molecules of interest. These aptamers are generated through iterative rounds of selection, amplification, and enrichment, ultimately yielding highly selective ligands. Secondly, the Pull-Down phase employs these aptamers to capture and isolate the target biomarkers from complex biological samples. This step ensures the specificity of the selected aptamers in binding to their intended targets. Lastly, mass spectrometry is utilized to identify and quantify the captured biomarkers, providing precise information about their presence and concentration in the sample. These quantitative data are invaluable in disease diagnosis and monitoring. Bio-SELEX’s significance lies in its ability to discover biomarkers for a wide range of diseases, spanning infectious and non-infectious conditions. This approach holds great promise for early disease detection, personalized medicine, and the development of targeted therapies. By harnessing the power of aptamers and mass spectrometry, Bio-SELEX advances our understanding of disease biology and opens new avenues for improved healthcare. MDPI 2023-11-11 /pmc/articles/PMC10660531/ /pubmed/37987356 http://dx.doi.org/10.3390/mps6060109 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Ospina-Villa, Juan David
Restrepo-Cano, Valentina
Sánchez-Jiménez, Miryan Margot
Bio-SELEX: A Strategy for Biomarkers Isolation Directly from Biological Samples
title Bio-SELEX: A Strategy for Biomarkers Isolation Directly from Biological Samples
title_full Bio-SELEX: A Strategy for Biomarkers Isolation Directly from Biological Samples
title_fullStr Bio-SELEX: A Strategy for Biomarkers Isolation Directly from Biological Samples
title_full_unstemmed Bio-SELEX: A Strategy for Biomarkers Isolation Directly from Biological Samples
title_short Bio-SELEX: A Strategy for Biomarkers Isolation Directly from Biological Samples
title_sort bio-selex: a strategy for biomarkers isolation directly from biological samples
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10660531/
https://www.ncbi.nlm.nih.gov/pubmed/37987356
http://dx.doi.org/10.3390/mps6060109
work_keys_str_mv AT ospinavillajuandavid bioselexastrategyforbiomarkersisolationdirectlyfrombiologicalsamples
AT restrepocanovalentina bioselexastrategyforbiomarkersisolationdirectlyfrombiologicalsamples
AT sanchezjimenezmiryanmargot bioselexastrategyforbiomarkersisolationdirectlyfrombiologicalsamples